Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domains

[1]  P. Várnai,et al.  Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains. , 2007, Molecular biology of the cell.

[2]  P. Várnai,et al.  Chaperone-mediated coupling of endoplasmic reticulum and mitochondrial Ca2+ channels , 2006, The Journal of cell biology.

[3]  Tobias Meyer,et al.  Rapid Chemically Induced Changes of PtdIns(4,5)P2 Gate KCNQ Ion Channels , 2006, Science.

[4]  T. Meyer,et al.  PI(3,4,5)P3 and PI(4,5)P2 Lipids Target Proteins with Polybasic Clusters to the Plasma Membrane , 2006, Science.

[5]  P. Várnai,et al.  Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells , 2006, The Journal of cell biology.

[6]  P. Várnai,et al.  Mitochondrial Ca2+ uptake with and without the formation of high-Ca2+ microdomains. , 2006, Cell calcium.

[7]  P. Parker,et al.  Compartmental signal modulation: Endosomal phosphatidylinositol 3-phosphate controls endosome morphology and selective cargo sorting , 2006, Proceedings of the National Academy of Sciences.

[8]  P. Várnai,et al.  Live cell imaging of phosphoinositide dynamics with fluorescent protein domains. , 2006, Biochimica et biophysica acta.

[9]  Mark Philips,et al.  Receptor Activation Alters Inner Surface Potential During Phagocytosis , 2006, Science.

[10]  Robbie Loewith,et al.  A pharmacological map of the PI3-K family defines a role for p110 alpha in signaling: The structure of complex of phosphoinositide 3- kinase gamma with inhibitor PIK-39 , 2006 .

[11]  Robbie Loewith,et al.  A Pharmacological Map of the PI3-K Family Defines a Role for p110α in Insulin Signaling , 2006, Cell.

[12]  G. Prestwich,et al.  Synthesis and biological activity of phospholipase C-resistant analogues of phosphatidylinositol 4,5-bisphosphate. , 2006, Journal of the American Chemical Society.

[13]  R. Irvine.  Nuclear inositide signalling -- expansion, structures and clarification. , 2006, Biochimica et biophysica acta.

[14]  L. Hunyady,et al.  Selective cellular effects of overexpressed pleckstrin-homology domains that recognize PtdIns(3,4,5)P3 suggest their interaction with protein binding partners , 2005, Journal of Cell Science.

[15]  P. Cullen,et al.  Coincidence detection in phosphoinositide signaling. , 2005, Trends in cell biology.

[16]  B. Adams.  Faculty Opinions recommendation of Phospholipase C in living cells: activation, inhibition, Ca2+ requirement, and regulation of M current. , 2005 .

[17]  L. Cantley,et al.  PtdIns(4,5)P2 Functions at the Cleavage Furrow during Cytokinesis , 2005, Current Biology.

[18]  A. Godi,et al.  The role of the phosphoinositides at the Golgi complex. , 2005, Biochimica et biophysica acta.

[19]  G. Halet.  Imaging phosphoinositide dynamics using GFP‐tagged protein domains , 2005, Biology of the cell.

[20]  Tobias Meyer,et al.  An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways , 2005, Nature Methods.

[21]  L. Hunyady,et al.  Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[22]  C. Downes,et al.  Probing phosphoinositide functions in signaling and membrane trafficking. , 2005, Trends in cell biology.

[23]  A. Miyawaki,et al.  Control of Calcium Signal Propagation to the Mitochondria by Inositol 1,4,5-Trisphosphate-binding Proteins* , 2005, Journal of Biological Chemistry.

[24]  P. Várnai,et al.  A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1. , 2005, Molecular biology of the cell.

[25]  M. Lemmon,et al.  Pleckstrin homology domains: not just for phosphoinositides. , 2004, Biochemical Society transactions.

[26]  D. Hilgemann.  New insights into the molecular and cellular workings of the cardiac Na+/Ca2+ exchanger. , 2004, American journal of physiology. Cell physiology.

[27]  T. Levine,et al.  Multiple Pools of Phosphatidylinositol 4-Phosphate Detected Using the Pleckstrin Homology Domain of Osh2p* , 2004, Journal of Biological Chemistry.

[28]  A. Prescott,et al.  Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates , 2004, The EMBO journal.

[29]  D. Lambright,et al.  Structural determinants of phosphoinositide selectivity in splice variants of Grp1 family PH domains , 2004, The EMBO journal.

[30]  Akihiko Tanimura,et al.  Fluorescent Biosensor for Quantitative Real-time Measurements of Inositol 1,4,5-Trisphosphate in Single Living Cells* , 2004, Journal of Biological Chemistry.

[31]  F. Gergely,et al.  Targeting of Inositol 1,4,5-Trisphosphate Receptors to the Endoplasmic Reticulum by Multiple Signals within Their Transmembrane Domains* , 2004, Journal of Biological Chemistry.

[32]  R. K. McEwen,et al.  Svp1p defines a family of phosphatidylinositol 3,5‐bisphosphate effectors , 2004, The EMBO journal.

[33]  L. Loew,et al.  A wave of IP3 production accompanies the fertilization Ca2+ wave in the egg of the frog, Xenopus laevis: theoretical and experimental support. , 2004, Cell calcium.

[34]  D. Alessi,et al.  FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P , 2004, Nature Cell Biology.

[35]  Diana Murray,et al.  Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. , 2004, Molecular cell.

[36]  C. Downes,et al.  Detection of novel intracellular agonist responsive pools of phosphatidylinositol 3,4-bisphosphate using the TAPP1 pleckstrin homology domain in immunoelectron microscopy. , 2004, The Biochemical journal.

[37]  P. Janmey,et al.  Phosphoinositide regulation of the actin cytoskeleton. , 2003, Annual review of physiology.

[38]  E. Pécheur,et al.  Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body. , 2003, Developmental cell.

[39]  M. Roth,et al.  Phosphatidylinositol 4 Phosphate Regulates Targeting of Clathrin Adaptor AP-1 Complexes to the Golgi , 2003, Cell.

[40]  R. Lefkowitz,et al.  Keeping G Proteins at Bay: A Complex Between G Protein-Coupled Receptor Kinase 2 and Gßγ , 2003, Science.

[41]  W. Zipfel,et al.  Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum , 2003, Nature Cell Biology.

[42]  R. Firtel,et al.  Receptor-mediated regulation of PI3Ks confines PI(3,4,5)P3 to the leading edge of chemotaxing cells. , 2003, Molecular biology of the cell.

[43]  T. Meyer,et al.  Single Cell Imaging of PI3K Activity and Glucose Transporter Insertion Into the Plasma Membrane by Dual Color Evanescent Wave Microscopy , 2003, Science's STKE.

[44]  Susan S. Taylor,et al.  A 15-Residue Bifunctional Element in d-AKAP1 Is Required for Both Endoplasmic Reticulum and Mitochondrial Targeting* , 2002, The Journal of Biological Chemistry.

[45]  P. Várnai,et al.  Inositol Lipid Binding and Membrane Localization of Isolated Pleckstrin Homology (PH) Domains , 2002, The Journal of Biological Chemistry.

[46]  D. Dormann,et al.  Visualizing PI3 Kinase-Mediated Cell-Cell Signaling during Dictyostelium Development , 2002, Current Biology.

[47]  L. Hunyady,et al.  Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor , 2002, The Journal of cell biology.

[48]  C. Downes,et al.  Subcellular localization of phosphatidylinositol 4,5-bisphosphate using the pleckstrin homology domain of phospholipase C delta1. , 2002, The Biochemical journal.

[49]  S. Munro,et al.  Targeting of Golgi-Specific Pleckstrin Homology Domains Involves Both PtdIns 4-Kinase-Dependent and -Independent Components , 2002, Current Biology.

[50]  K. Mikoshiba,et al.  A Novel Recombinant Hyperaffinity Inositol 1,4,5-Trisphosphate (IP3) Absorbent Traps IP3, Resulting in Specific Inhibition of IP3-mediated Calcium Signaling* , 2002, The Journal of Biological Chemistry.

[51]  D. Alessi,et al.  Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo , 2002 .

[52]  T. Takenawa,et al.  Phosphoinositides, key molecules for regulation of actin cytoskeletal organization and membrane traffic from the plasma membrane. , 2001, Biochimica et biophysica acta.

[53]  Paul Tempst,et al.  PtdIns(3)P regulates the neutrophil oxidase complex by binding to the PX domain of p40phox , 2001, Nature Cell Biology.

[54]  M. Yaffe,et al.  The PX domains of p47phox and p40phox bind to lipid products of PI(3)K , 2001, Nature Cell Biology.

[55]  L Shapiro,et al.  G-Protein Signaling Through Tubby Proteins , 2001, Science.

[56]  R. Habets,et al.  Monitoring Agonist-induced Phospholipase C Activation in Live Cells by Fluorescence Resonance Energy Transfer* , 2001, The Journal of Biological Chemistry.

[57]  S. McLaughlin,et al.  The Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Binds Strongly to Phosphatidylinositol 4,5-Bisphosphate* , 2001, The Journal of Biological Chemistry.

[58]  P R Evans,et al.  Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. , 2001, Science.

[59]  J. Holik,et al.  Distinct Polyphosphoinositide Binding Selectivities for Pleckstrin Homology Domains of GRP1-like Proteins Based on DiglycineVersus Triglycine Motifs* , 2000, The Journal of Biological Chemistry.

[60]  Joseph C. Shope,et al.  Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[61]  S. Dowler,et al.  Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities. , 2000, The Biochemical journal.

[62]  M. Lindsay,et al.  Localization of phosphatidylinositol 3‐phosphate in yeast and mammalian cells , 2000, The EMBO journal.

[63]  P. Várnai,et al.  How accurately can we image inositol lipids in living cells? , 2000, Trends in pharmacological sciences.

[64]  M. Caron,et al.  Mutational Analysis of Gβγ and Phospholipid Interaction with G Protein-coupled Receptor Kinase 2* , 2000, The Journal of Biological Chemistry.

[65]  D. Lambright,et al.  The FYVE Domain of Early Endosome Antigen 1 Is Required for Both Phosphatidylinositol 3-Phosphate and Rab5 Binding , 2000, The Journal of Biological Chemistry.

[66]  V. Ferrans,et al.  Similarities in Function and Gene Structure of Cytohesin-4 and Cytohesin-1, Guanine Nucleotide-exchange Proteins for ADP-ribosylation Factors* , 2000, The Journal of Biological Chemistry.

[67]  Tobias Meyer,et al.  Phosphatidylinositol 4,5-Bisphosphate Functions as a Second Messenger that Regulates Cytoskeleton–Plasma Membrane Adhesion , 2000, Cell.

[68]  Alexander G. Gray,et al.  The pleckstrin homology domains of protein kinase B and GRP1 (general receptor for phosphoinositides-1) are sensitive and selective probes for the cellular detection of phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 3,4,5-trisphosphate in vivo. , 1999, The Biochemical journal.

[69]  D. McCormick,et al.  Essential Role of Phosphoinositide Metabolism in Synaptic Vesicle Recycling , 1999, Cell.

[70]  D. Burdakov,et al.  Polarity in intracellular calcium signaling. , 1999, BioEssays : news and reviews in molecular, cellular and developmental biology.

[71]  J. Brugge,et al.  Controlled Dimerization of ErbB Receptors Provides Evidence for Differential Signaling by Homo- and Heterodimers , 1999, Molecular and Cellular Biology.

[72]  J. Tavaré,et al.  EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain. , 1999, Journal of cell science.

[73]  C. Burd,et al.  Phosphatidylinositol 3-phosphate recognition by the FYVE domain. , 1999, Molecular cell.

[74]  Julian Downward,et al.  Akt/PKB localisation and 3′ phosphoinositide generation at sites of epithelial cell–matrix and cell–cell interaction , 1999, Current Biology.

[75]  P. Várnai,et al.  Phosphatidylinositol 3-Kinase-dependent Membrane Association of the Bruton’s Tyrosine Kinase Pleckstrin Homology Domain Visualized in Single Living Cells* , 1999, The Journal of Biological Chemistry.

[76]  S. Cockcroft.  Mammalian phosphatidylinositol transfer proteins: emerging roles in signal transduction and vesicular traffic. , 1999, Chemistry and physics of lipids.

[77]  J. Tavaré,et al.  Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain. , 1998, The Biochemical journal.

[78]  W. Kolanus,et al.  The PH domain and the polybasic c domain of cytohesin-1 cooperate specifically in plasma membrane association and cellular function. , 1998, Molecular biology of the cell.

[79]  Marino Zerial,et al.  EEA1 links PI(3)K function to Rab5 regulation of endosome fusion , 1998, Nature.

[80]  C. Burd,et al.  Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. , 1998, Molecular cell.

[81]  J. York,et al.  Tyrosine 1101 of Tie2 Is the Major Site of Association of p85 and Is Required for Activation of Phosphatidylinositol 3-Kinase and Akt , 1998, Molecular and Cellular Biology.

[82]  S. Munro,et al.  The pleckstrin homology domain of oxysterol-binding protein recognises a determinant specific to Golgi membranes , 1998, Current Biology.

[83]  J. Tavaré,et al.  Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase , 1998, Current Biology.

[84]  Tobias Meyer,et al.  Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells , 1998, Current Biology.

[85]  Péter Várnai,et al.  Visualization of Phosphoinositides That Bind Pleckstrin Homology Domains: Calcium- and Agonist-induced Dynamic Changes and Relationship to Myo-[3H]inositol-labeled Phosphoinositide Pools , 1998, The Journal of cell biology.

[86]  L. Cantley,et al.  A Comparative Analysis of the Phosphoinositide Binding Specificity of Pleckstrin Homology Domains* , 1997, The Journal of Biological Chemistry.

[87]  J. Holik,et al.  Signaling by Phosphoinositide-3,4,5-Trisphosphate Through Proteins Containing Pleckstrin and Sec7 Homology Domains , 1997, Science.

[88]  David R. Kaplan,et al.  Direct Regulation of the Akt Proto-Oncogene Product by Phosphatidylinositol-3,4-bisphosphate , 1997, Science.

[89]  E. Querfurth,et al.  Distinct specificity in the recognition of phosphoinositides by the pleckstrin homology domains of dynamin and Bruton's tyrosine kinase. , 1996, The EMBO journal.

[90]  H. Takeuchi,et al.  Localization of a high-affinity inositol 1,4,5-trisphosphate/inositol 1,4,5,6-tetrakisphosphate binding domain to the pleckstrin homology module of a new 130 kDa protein: characterization of the determinants of structural specificity. , 1996, The Biochemical journal.

[91]  K. Mikoshiba,et al.  Mutational Analysis of the Ligand Binding Site of the Inositol 1,4,5-Trisphosphate Receptor* , 1996, The Journal of Biological Chemistry.

[92]  P. Sigler,et al.  Specific and high-affinity binding of inositol phosphates to an isolated pleckstrin homology domain. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[93]  P. Crespo,et al.  Dual Effect of β-Adrenergic Receptors on Mitogen-activated Protein Kinase , 1995, The Journal of Biological Chemistry.

[94]  T. Tanaka,et al.  Activation Induces Dephosphorylation of Cofilin and Its Translocation to Plasma Membranes in Neutrophil-like Differentiated HL-60 Cells (*) , 1995, The Journal of Biological Chemistry.

[95]  György Hajnóczky,et al.  Decoding of cytosolic calcium oscillations in the mitochondria , 1995, Cell.

[96]  M. Fukuda,et al.  Golgi Retention Mechanism of -1,4-Galactosyltransferase , 1995, The Journal of Biological Chemistry.

[97]  T. Takenawa,et al.  ATP-dependent inositide phosphorylation required for Ca2+-activated secretion , 1995, Nature.

[98]  R. Kahn,et al.  GTP hydrolysis by ADP-ribosylation factor is dependent on both an ADP-ribosylation factor GTPase-activating protein and acid phospholipids. , 1994, The Journal of biological chemistry.

[99]  D. Eberhard,et al.  Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP. , 1990, The Biochemical journal.

[100]  L. Cantley,et al.  Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate , 1988, Nature.

[101]  M. Monaco,et al.  The relationship of hormone-sensitive and hormone-insensitive phosphatidylinositol to phosphatidylinositol 4,5-bisphosphate in the WRK-1 cell. , 1986, The Journal of biological chemistry.

[102]  Michael J. Berridge,et al.  Inositol trisphosphate, a novel second messenger in cellular signal transduction , 1984, Nature.

[103]  G. Marinetti,et al.  Transforming protein of avian sarcoma virus UR2 is associated with phosphatidylinositol kinase activity: possible role in tumorigenesis. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[104]  P. Hawkins,et al.  Analysis of the metabolic turnover of the individual phosphate groups of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. Validation of novel analytical techniques by using 32P-labelled lipids from erythrocytes. , 1984, The Biochemical journal.

[105]  R. Michell.  Inositol phospholipids and cell surface receptor function. , 1975, Biochimica et biophysica acta.

[106]  C. Ballou,et al.  Myo-inositol phosphates from beef brain phosphoinostide. , 1960, The Journal of biological chemistry.

[107]  L. Hokin,et al.  Enzyme secretion and the incorporation of P32 into phospholipides of pancreas slices. , 1953, The Journal of biological chemistry.

[108]  T. Mak,et al.  Control of cell polarity and motility by the PtdIns(3,4,5)P3 phosphatase SHIP1 , 2007, Nature Cell Biology.

[109]  M. Lemmon,et al.  Phosphatidylinositol 3,5-bisphosphate: metabolism and cellular functions. , 2006, Trends in biochemical sciences.

[110]  D. Alessi,et al.  Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo. , 2002, The Biochemical journal.

[111]  M. Caron,et al.  Mutational analysis of Gbetagamma and phospholipid interaction with G protein-coupled receptor kinase 2. , 2000, The Journal of biological chemistry.

[112]  M. Fukuda,et al.  Golgi retention mechanism of beta-1,4-galactosyltransferase. Membrane-spanning domain-dependent homodimerization and association with alpha- and beta-tubulins. , 1995, The Journal of biological chemistry.

[113]  P. Crespo,et al.  Dual effect of beta-adrenergic receptors on mitogen-activated protein kinase. Evidence for a beta gamma-dependent activation and a G alpha s-cAMP-mediated inhibition. , 1995, The Journal of biological chemistry.

[114]  T. Mak,et al.  Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis , 2022 .