A quantitative study on the nigro-neostriatal dopamine neuron system in the rat.

In a combined biochemical and histochemical investigation the following quantitative data on a unilateral nigro-neostriatal dopamine neuron system in the rat have been obtained or calculated. — The number of neurons is 3,500 on the average. — There are about 120 pg dopamine per neuron (1 pg = 10-12 g). — The terminals contain 50 (or 150) times more dopamine than the cell bodies. —The terminal system of one neuron varies mainly between 55 and 77 cm in length and contains about 500,000 varicosities. — The neostriatum, with a volume of 33 mm3, contains 109 dopamine varicosities occupying 0.3 % of the volume, and 4,000,000 nerve cell bodies (not containing dopamine). — One average dopamine varicosity, with a diameter of 0.4 μ, contains 2.5 × 10-4 pg dopamine, and has a dopamine concentration around 8,000 μg/g. — One dopamine cell body of the substantia nigra, with a mean diameter of 30 μ, contains 2.5 (or 0.8) pg dopamine, and has a dopamine concentration around 200 (or 60) μg/g.

[1]  B. Hamberger,et al.  Localization of catecholamine uptake in rat brain slices. , 2009, Acta pharmacologica et toxicologica.

[2]  Å. Bertler,et al.  SOEME OBSERVATIONS ON ADRENERGIC CONNECTIONS BETWEEN MESENCEPHALON AND CEREBRAL HEMISPHERES. , 2009, Acta pharmacologica et toxicologica.

[3]  Lars Olson,et al.  Ascending Monoamine Neurons to the Telencephalon and Diencephalon , 1966 .

[4]  T. Hökfelt,et al.  The noradrenaline content of the varicosities of sympathetic adrenergic nerve terminals in the rat. , 1966, Acta physiologica Scandinavica.

[5]  A. Dahlström,et al.  Some quantitative studies on the noradrenaline content in the cell bodies and terminals of a sympathetic adrenergic neuron system. , 1966, Acta physiologica Scandinavica.

[6]  T. Hökfelt.  A MODIFICATION OF THE HISTOCHEMICAL FLUORESCENCE METHOD FOR THE DEMONSTRATION OF CATECHOLAMINES AND 5-HYDROXY-TRYPTAMINE, USING ARALDITE AS EMBEDDING MEDIUM , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[7]  K. Fuxe,et al.  Mapping out of catecholamine and 5-hydroxytryptamine neurons innervating the telencephalon and diencephalon. , 1965, Life sciences.

[8]  G. Thieme,et al.  SMALL TISSUE DRYERS WITH HIGH CAPACITY FOR RAPID FREEZE-DRYING , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[9]  B. Hamberger,et al.  STANDARDIZATION OF PARAFORMALDEHYDE AND OF CERTAIN PROCEDURES FOR THE HISTOCHEMICAL DEMONSTRATION OF CATECHOLAMINES , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  C J CLEMEDSON,et al.  DYNAMIC RESPONSE OF CHEST WALL AND LUNG INJURIES IN RABBITS EXPOSED TO AIR SHOCK WAVES OF SHORT DURATION. , 1964, Acta physiologica Scandinavica. Supplementum.

[11]  K. Fuxe,et al.  DEMONSTRATION AND MAPPING OUT OF NIGRO-NEOSTRIATAL DOPAMINE NEURONS. , 1964, Life sciences.

[12]  Å. Bertler.  Occurrence and Localization of Catechol Amines in the Human Brain , 1961 .

[13]  G. Thieme,et al.  Adenosinephosphates in the rat adrenal medulia. I. Adrenal medulla in minimal secretory activity. , 1959, Acta physiologica Scandinavica.

[14]  A. Carlsson,et al.  A fluorimetric method for the determination of dopamine (3-hydroxytyramine). , 1958, Acta physiologica Scandinavica.

[15]  N. Hillarp,et al.  Release of adenosine triphosphate along with adrenaline and noradrenaline following stimulation of the adrenal medulla. , 1956, Acta physiologica Scandinavica.

[16]  S. W. Ranson,et al.  On the number of nerve cells in the ganglion cervicale superius and of nerve fibers in the cephalic end of the truncus sympathicus in the cat and on the numerical relations of preganglionic and postganglionic neurones , 1918 .

[17]  K. Fuxe,et al.  EVIDENCE FOR THE EXISTENCE OF MONOAMINE NEURONS IN THE CENTRAL NERVOUS SYSTEM. IV. DISTRIBUTION OF MONOAMINE NERVE TERMINALS IN THE CENTRAL NERVOUS SYSTEM. , 1965, Acta physiologica Scandinavica. Supplementum.

[18]  K. Fuxe,et al.  FURTHER EVIDENCE FOR THE PRESENCE OF NIGRO-NEOSTRIATAL DOPAMINE NEURONS IN THE RAT. , 1965, The American journal of anatomy.

[19]  K. Fuxe,et al.  CELLULAR LOCALIZATION OF MONOAMINES IN THE SPINAL CORD. , 1964, Acta physiologica Scandinavica.

[20]  J. V. Euw,et al.  Fluoreszenzenzmethoden zur histochemischen Sichtbarmachung von Monoaminen. 2.. Identifizierung des fluoreszierenden Produktes aus Dopamin und Formaldehyd , 1964 .

[21]  K. Fuxe,et al.  EVIDENCE FOR THE EXISTENCE OF MONOAMINE-CONTAINING NEURONS IN THE CENTRAL NERVOUS SYSTEM. I. DEMONSTRATION OF MONOAMINES IN THE CELL BODIES OF BRAIN STEM NEURONS. , 1964, Acta physiologica Scandinavica. Supplementum.

[22]  H. Corrodi,et al.  Fluoreszenzmethoden zur histochemischen Sichtbarmachung von Monoaminen. 1. Identifizierung der fluoreszierenden Produkte aus Modellversuchen mit 6,7‐Dimethoxyisochinolinderivaten und Formaldehyd , 1963 .

[23]  B. Falck,et al.  Cellular localization of brain monoamines. , 1962, Acta physiologica Scandinavica. Supplementum.

[24]  A. Carlsson,et al.  In‐Vivo Decarboxylation of α‐Methyl DOPA and α‐Methyl Metatyrosine , 1962 .

[25]  R. Eliasson.  Studies on prostaglandin; occurrence, formation and biological actions. , 1959, Acta physiologica Scandinavica. Supplementum.