Ion conducting polymer microelectrodes for interfacing with neural networks

We have examined the stimulation and recording properties of conjugated polymer microelectrode arrays as interfaces with neural networks of dissociated cortical cells. In particular the stimulation properties were investigated as a means of supplying a neural network with information. The stimulation efficiency at low stimulation voltages was evaluated and referenced to bare indium tin oxide (ITO) electrodes. The polymer electrodes were electrochemically polymerized from a blend of poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT-PSS) and ethylenedioxythiophene (EDOT) onto ITO microelectrodes. Dissociated cortical cells were then plated on the electrodes and cultivated to form neural networks. Polymer electrode stimulation evoked a much greater response from the network than stimulation from ITO electrodes. Neural interfaces using polymer electrodes could be maintained for several months.

[1]  David C. Martin,et al.  Conducting polymers grown in hydrogel scaffolds coated on neural prosthetic devices. , 2004, Journal of biomedical materials research. Part A.

[2]  David C. Martin,et al.  Electrochemical deposition and characterization of poly(3,4-ethylenedioxythiophene) on neural microelectrode arrays , 2003 .

[3]  T.L. Rose,et al.  Electrical stimulation with Pt electrodes. VIII. Electrochemically safe charge injection limits with 0.2 ms pulses (neuronal application) , 1990, IEEE Transactions on Biomedical Engineering.

[4]  O. Inganäs,et al.  Conducting Polymer Hydrogels as 3D Electrodes: Applications for Supercapacitors , 1999 .

[5]  Markus Meister,et al.  Multi-neuronal signals from the retina: acquisition and analysis , 1994, Journal of Neuroscience Methods.

[6]  G J Brewer,et al.  Modulation of neural network activity by patterning. , 2001, Biosensors & bioelectronics.

[7]  John M. Beggs,et al.  Behavioral / Systems / Cognitive Neuronal Avalanches Are Diverse and Precise Activity Patterns That Are Stable for Many Hours in Cortical Slice Cultures , 2004 .

[8]  H. Robinson,et al.  Spontaneous periodic synchronized bursting during formation of mature patterns of connections in cortical cultures , 1996, Neuroscience Letters.

[9]  O. Inganäs,et al.  Electrochemical Characterization of Poly(3,4‐ethylene dioxythiophene) Based Conducting Hydrogel Networks , 2000 .

[10]  F. Rattay,et al.  Analysis of the electrical excitation of CNS neurons , 1998, IEEE Transactions on Biomedical Engineering.

[11]  Stephen Britland,et al.  Morphogenetic guidance cues can interact synergistically and hierarchically in steering nerve cell growth , 1996 .

[12]  E. Zrenner,et al.  Can subretinal microphotodiodes successfully replace degenerated photoreceptors? , 1999, Vision Research.

[13]  Akio Kawana,et al.  Recognition of artificial microstructures by sensory nerve fibers in culture , 1988, Brain Research.

[14]  J. Hetke,et al.  Surface modification of neural recording electrodes with conducting polymer/biomolecule blends. , 2001, Journal of biomedical materials research.

[15]  Olle Inganäs,et al.  Polymer Hydrogel Microelectrodes for Neural Communication , 2002 .

[16]  M. Raastad,et al.  Unmyelinated axons in the rat hippocampus hyperpolarize and activate an H current when spike frequency exceeds 1 Hz , 2003, The Journal of physiology.

[17]  Yasuhiko Jimbo,et al.  A system for MEA-based multisite stimulation , 2003, IEEE Transactions on Biomedical Engineering.

[18]  G. Gross,et al.  Multielectrode analysis of coordinated, multisite, rhythmic bursting in cultured CNS monolayer networks , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[19]  Steve M. Potter,et al.  Effective parameters for stimulation of dissociated cultures using multi-electrode arrays , 2004, Journal of Neuroscience Methods.

[20]  Yasuhiko Jimbo,et al.  The dynamics of a neuronal culture of dissociated cortical neurons of neonatal rats , 2000, Biological Cybernetics.

[21]  T. Stieglitz,et al.  A biohybrid system to interface peripheral nerves after traumatic lesions: design of a high channel sieve electrode. , 2002, Biosensors & bioelectronics.

[22]  Hitoshi Yamato,et al.  Stability of polypyrrole and poly(3,4-ethylenedioxythiophene) for biosensor application , 1995 .

[23]  H. Robinson,et al.  Simultaneous induction of pathway-specific potentiation and depression in networks of cortical neurons. , 1999, Biophysical journal.