Eye irritation responses in rabbit and man after single applications of equal volumes of undiluted model liquid detergent products.

A criticism of the use of the rabbit low-volume eye test to determine eye irritation hazard for man is the lack of comparative data in man and rabbit with undiluted products. To address this, such a study has been performed in man and rabbit using undiluted model liquid detergents. The hypothesis tested was that if, under identical test conditions, the effects in the rabbit were the same or greater than the response in man, then it is valid to use the low-volume eye test to assess eye irritation hazard for man. The studies were carried out using 29 human volunteers and 12 rabbits. The effects in the rabbit were unequivocally greater than the effects observed in man, but clearly less than the expected response from these types of product in the Draize test. The results from this study confirm the sensitivity of the rabbit as a test species, and support the use of the low-volume eye test to assess eye irritation hazard for man. Any in vitro/ex vivo alternative to assess eye irritation should be developed against the rabbit low-volume eye test or human data where available.

[1]  F. E. Freeberg,et al.  Correlation of Animal Test Methods with Human Experience for Household Products , 1984 .

[2]  F. E. Freeberg,et al.  Human and rabbit eye responses to chemical insult. , 1986, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[3]  L. Rubin,et al.  Use of the rhesus monkey for predicting human response to eye irritants. , 1969, Toxicology and applied pharmacology.

[4]  M. Farage,et al.  Use of Clinical and Consumer Eye Irritation Data to Evaluate the Low-Volume Eye Test , 1995 .

[5]  John H. Draize,et al.  METHODS FOR THE STUDY OF IRRITATION AND TOXICITY OF SUBSTANCES APPLIED TOPICALLY TO THE SKIN AND MUCOUS MEMBRANES , 1944 .

[6]  J F Griffith,et al.  Dose-response studies with chemical irritants in the albino rabbit eye as a basis for selecting optimum testing conditions for predicting hazard to the human eye. , 1980, Toxicology and applied pharmacology.

[7]  D. T. Hooker,et al.  Correlation of Animal Eye Test Data with Human Experience for Household Products: An Update , 1986 .

[8]  R N Hill,et al.  The use of low-volume dosing in the eye irritation test. , 1993, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[9]  W Steiling,et al.  A critical review of the assessment of eye irritation potential using the draize rabbit eye test , 1998, Journal of applied toxicology : JAT.

[10]  J. Beckley.  Comparative eye testing: Man vs. animal , 1965 .