Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC)--measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts.

The analysis of PCBs in fish tissues by immunoassay methods was evaluated using fish collected from a US monitoring program, the National Contaminant Biomonitoring Program of the US Department of Interior, Fish and Wildlife Service. Selected composite whole fish samples, which represented widely varying concentrations and sources of PCBs, were extracted and subjected to congener PCB analysis by gas chromatography (GC) and total PCB analysis using an ELISA (ePCBs) calibrated against technical Aroclor 1248. PCB congener patterns in these fishes were different from the patterns found in commercial Aroclors or their combinations as demonstrated by principal component analysis of normalized GC congener data. The sum of the PCB congeners measured by GC (total-PCBs) ranged from 37 to 4600 ng/g (wet weight). Concentrations of PCBs as determined by the ELISA method were positively correlated with total-PCBs and the ePCBs/total-PCBs ratios for individual samples ranged from 1 to 6. Ratios of ePCBs/total-PCBs for dilutions of Aroclors 1242, 1254, and 1260 and for matrix spikes range from 0.6 for 1242 to 2.5 for 1254 and 1260. These results suggest that higher chlorinated PCB congeners have higher affinity for the anti-PCB antibodies. Partial least squares with latent variable analysis of GC and ELISA data of selected Aroclors and fish samples also support the conclusion that ELISA derived PCB concentrations are dependent on the degree of chlorination.

[1]  M. Romkes,et al.  High-resolution PCB analysis: synthesis and chromatographic properties of all 209 PCB congeners. , 1984, Environmental science & technology.

[2]  B. Watkins,et al.  An immunoassay for pyrethroids : detection of permethrin in meat , 1989 .

[3]  Fernando M. Rubio,et al.  Quantitative determination of PCBs in soil and water by a magnetic particle-based immunoassay , 1996 .

[4]  Bruce R. Kowalski,et al.  Chemometrics, mathematics and statistics in chemistry , 1984 .

[5]  D. Stalling,et al.  Are polychlorinated biphenyl residues adequately described by Aroclor mixture equivalents? Isomer-specific principal components analysis of such residues in fish and turtles , 1987 .

[6]  W. Shiu,et al.  Monoaromatic hydrocarbons, chlorobenzenes and PCBs , 1992 .

[7]  Cyrus Chothia,et al.  The accessible surface area and stability of oligomeric proteins , 1987, Nature.

[8]  J. L. Zajicek,et al.  National Pesticide Monitoring Program: Residues of organochlorine chemicals in freshwater fish, 1980–81 , 1985, Archives of environmental contamination and toxicology.

[9]  R. E. Carlson.  Hapten Versus Competitor Design Strategies for Immunoassay Development , 1995 .

[10]  K. Ballschmiter,et al.  The determination of chlorinated biphenyls, chlorinated dibenzodioxins, and chlorinated dibenzofurans by GC‐MS , 1992 .

[11]  J. L. Zajicek,et al.  Application of enzyme-linked immunosorbent assay for measurement of polychlorinated biphenyls from hydrophobic solutions: Extracts of fish and dialysates of semipermeable membrane devices: Chapter 26 , 1996 .

[12]  J. L. Zajicek,et al.  National contaminant biomonitoring program: Residues of organochlorine chemicals in U.S. Freshwater Fish, 1976–1984 , 1990, Archives of environmental contamination and toxicology.

[13]  S. Safe,et al.  Polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and related compounds: environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs). , 1990, Critical reviews in toxicology.

[14]  C Chothia,et al.  Surface, subunit interfaces and interior of oligomeric proteins. , 1988, Journal of molecular biology.

[15]  K. Ballschmiter,et al.  Analysis of polychlorinated biphenyls (PCB) by glass capillary gas chromatography , 1980 .

[16]  W. Shiu,et al.  Illustrated handbook of physical-chemical properties and environmental fate for organic chemicals. Volume 5: pesticide chemicals. , 1992 .

[17]  Linda S. Birnbaum,et al.  Toxic equivalency factors for dioxin-like PCBs , 1994 .

[18]  N. Melnychuk,et al.  Rapid Analysis of PCBs in Soil by Enzyme Immunoassay , 1995 .

[19]  P. Albro,et al.  The use of radioimmunoassay for the detection of polychlorinated dibenzo-p-dioxins in fish samples , 1989 .

[20]  C Rappe,et al.  A toxic equivalency factor scale for polychlorinated dibenzofurans. , 1994, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[21]  T. Schwartz,et al.  Polychlorinated biphenyl congeners in emergent mayflies from the upper Mississippi river. , 1994, Environmental science & technology.

[22]  Annika Hanberg,et al.  Toxic equivalency factors for dioxin-like PCBs , 1994, Environmental science and pollution research international.

[23]  S. Wold,et al.  Multivariate Data Analysis in Chemistry , 1984 .

[24]  C. Richter,et al.  Immunoassay monitoring of polychlorinated biphenyls (PCBs) in the Great Lakes , 1994, Environmental science and pollution research international.

[25]  C. Tanford,et al.  Interfacial free energy and the hydrophobic effect. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[26]  J. Butler.  Immunochemistry of solid-phase immunoassay , 1991 .

[27]  A. Fersht,et al.  Contribution of hydrophobic interactions to protein stability , 1988, Nature.

[28]  Mitchell D. Erickson,et al.  Analytical Chemistry of PCBs , 1986 .