Screening of Hyaluronidase Inhibitor in Korean Medicinal Plants

Mammalian hyaluronidases (HAase, EC 3.2.1.35) are a family of enzymes that hydrolyse N-acetyl-D-glucosamine (1-4) glycosidic bonds in hyaluronic acid, which is found in skin, cartilage, and the vitreous body. Although HAase is generally present in an inactive form within subcellular lysosomes, it is released in an active form in some types of inflammation and tissue injuries, thereby contributing to the inflammatory response. The HAase inhibitory activity of 500 methanolic extracts of 500 species from medicinal plants was screened using a Morgan microplate assay. The viscosity of the hyaluronic acid was measured with an Ubbelohde viscometer. Three MeOH extracts inhibited more than 50% of HAase activity at a concentration of 2 mg/ml. HAase inhibitory rates (%) of three species of medicinal plant extracts, Styrax japonica, Deutzia coreana, and Osmanthus insularis were 57.28%, 53.50%, and 53.19%, respectively. The rate of HAase inhibition of the extracts was dose dependent. In the HAase inhibitory assay using the Ubbelohde viscometer, the results were in good agreement with the results from the Morgan assay. The results suggest that HAase inhibitory compounds extracted from the stem of S. japonica, D. coreana, and O. insularis might be multifunctional and prevent the degradation of hyaluronic acid and the induction of allergic reactions and inflammation.

[1]  Woo-Jin Kim,et al.  Anti-cancer Activity of Styrax japonica Bark Extrats , 2014 .

[2]  Riyaz Mohamed,et al.  Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark , 2012, Natural product research.

[3]  K. Lim,et al.  Inhibitory effect of Styrax Japonica Siebold et al. Zuccarini glycoprotein (38 kDa) on interleukin-1β and induction proteins in chromium(VI)-treated BNL CL.2 cells , 2012, Molecular and Cellular Biochemistry.

[4]  K. Girish,et al.  Anti-venom potential of aqueous extract of stem bark of Mangifera indica L. against Daboia russellii (Russell's viper) venom. , 2011, Indian journal of biochemistry & biophysics.

[5]  K. Lim,et al.  Apoptotic activity of ethanol extract from Styrax Japonica Siebold et al Zuccarini in HepG2 cells. , 2010, Journal of ethnopharmacology.

[6]  R. Puy,et al.  Hyaluronidase allergy: A rare cause of periorbital inflammation , 2010, The Australasian journal of dermatology.

[7]  K. Girish,et al.  The magic glue hyaluronan and its eraser hyaluronidase: a biological overview. , 2007, Life sciences.

[8]  M. Simpson,et al.  Hyaluronidase expression induces prostate tumor metastasis in an orthotopic mouse model. , 2006, The American journal of pathology.

[9]  F. Duru,et al.  Aspirin augments hyaluronidase induced adhesion inhibition , 2004 .

[10]  M. Kinoshita,et al.  Hyaluronic acid: separation and biological implications. , 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[11]  E. Menzel,et al.  Hyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses. , 1998, Cancer letters.

[12]  G. Frost,et al.  The Hyaluronidases: A Chemical, Biological and Clinical Overview. , 1996 .

[13]  G. Kreil.  Hyaluronidases — a group of neglected enzymes , 1995, Protein science : a publication of the Protein Society.

[14]  T. Satoh,et al.  Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic actions. , 1992, Chemical & pharmaceutical bulletin.

[15]  I. Hampson,et al.  Angiogenesis induced by degradation products of hyaluronic acid. , 1985, Science.

[16]  B. T. Storey,et al.  Binding of mouse spermatozoa to the zonae pellucidae of mouse eggs in cumulus: evidence that the acrosomes remain substantially intact. , 1984, Biology of reproduction.

[17]  L. A. Thet,et al.  Changes in lung hyaluronidase activity associated with lung growth, injury and repair. , 1983, Biochemical and biophysical research communications.

[18]  B. Toole,et al.  Hyaluronate and hyaluronidase in the developing chick embryo kidney. , 1983, Cell differentiation.

[19]  R. Donoff,et al.  Hyaluronidase activity during open wound healing in rabbits: a preliminary report. , 1978, The Journal of surgical research.

[20]  B. Toole,et al.  The extracellular matrix of the regenerating newt limb: synthesis and removal of hyaluronate prior to differentiation. , 1971, Developmental biology.

[21]  Steffek Aj,et al.  Hyaluronidase activity of human gingiva. , 1968 .

[22]  E. Salvati,et al.  Advantages of local over spinal anesthesia in anorectal surgery. , 1957, Surgery, gynecology & obstetrics.

[23]  Kratzer Gl,et al.  Advantages of local over spinal anesthesia in anorectal surgery. , 1956 .

[24]  M. Rapport,et al.  Isolation of a Crystalline Disaccharide, Hyalobiuronic Acid, from Hyaluronic Acid , 1951, Nature.

[25]  K. Meyer.  The biological significance of hyaluronic acid and hyaluronidase. , 1947, Physiological reviews.

[26]  Karl Meyer,et al.  THE POLYSACCHARIDE OF THE VITREOUS HUMOR , 1934 .

[27]  Ji-Yeon Kim,et al.  Styraxoside A isolated from the stem bark of Styrax japonica inhibits lipopolysaccharide-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells by suppressing nuclear factor-kappa B activation. , 2007, Biological & pharmaceutical bulletin.

[28]  U. S. Singh,et al.  Studies on antifertility effects of gossypol acetic acid in domestic cocks. , 1989, Journal of reproduction and fertility.

[29]  T. Laurent,et al.  The properties and turnover of hyaluronan. , 1986, Ciba Foundation symposium.

[30]  R. McRORIE,et al.  Biochemistry of mammalian fertilization. , 1974, Annual review of biochemistry.

[31]  H. Fullmer,et al.  Hyaluronidase activity of human gingiva. , 1968, Archives of pathology.