Neuroprotection with Bcl-220–34 peptide against trauma

WE tested the neuroprotective potential of the Bcl-220–34 peptide sequence in hippocampal slices. Treatment with Bcl-2 after fluid percussion trauma significantly improved recovery of CA1 antidromic PS to a mean of 92% ± 1 of initial amplitude, compared with only 16% ± 2 in unmedicated slices. The EC50 for trauma protection was 84 μM Bcl-220–34. Protection with Bcl-220–34 also extended to long-term potentiation. No protection was seen with the reverse sequence of Bcl-220–34. Treatment with Bcl-220–34 also protected against hypoxic damage, with treated slices recovering to 98% ± 2, while unmedicated slices recovered to 14% ± 2. Similar protection was seen against AMPA, NMDA and nitric oxide. These findings indicate that Bcl-220–34 provides specific neuroprotection against acute CA1 neuronal injury.

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