Molecular and structural analyses of a novel temperature stress‐induced lipocalin from wheat and Arabidopsis

Two cDNAs corresponding to a novel lipocalin were identified from wheat and Arabidopsis. The two cDNAs designated Tatil for Triticum aestivum L. temperature‐induced lipocalin and Attil for Arabidopsis thaliana temperature‐induced lipocalin encode polypeptides of 190 and 186 amino acids respectively. Structure analyses indicated the presence of the three structurally conserved regions that characterize lipocalins. Sequence analyses revealed that this novel class of plant lipocalin shares homology with three evolutionarily related lipocalins: the mammalian apolipoprotein D (ApoD), the bacterial lipocalin and the insect Lazarillo. The comparison of the putative tertiary structures of both the human ApoD and the wheat TaTIL suggest that the two proteins differ in membrane attachment and ligand interaction. Northern analyses demonstrated that Tatil and Attil transcripts are upregulated during cold acclimation and heat‐shock treatment. The putative functions of this novel class of plant lipocalins during temperature stresses are discussed.

[1]  B. de Kruyff,et al.  The function of sterols in membranes. , 1976, Biochimica et biophysica acta.

[2]  É. Carpentier,et al.  Identification and characterization of a low temperature regulated gene encoding an actin‐binding protein from wheat , 1996, FEBS letters.

[3]  Y. Nakamura,et al.  Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones. , 2000, DNA research : an international journal for rapid publication of reports on genes and genomes.

[4]  T. Attwood,et al.  Structure and sequence relationships in the lipocalins and related proteins , 1993, Protein science : a publication of the Protein Society.

[5]  D R Flower,et al.  The lipocalin protein family: structure and function. , 1996, The Biochemical journal.

[6]  G. A. Thompson,et al.  Evidence for a glycosylinositolphospholipid-anchored alkaline phosphatase in the aquatic plant Spirodela oligorrhiza. , 1996, Biochimica et biophysica acta.

[7]  Geoffrey J. Barton,et al.  JPred : a consensus secondary structure prediction server , 1999 .

[8]  S. Clouse,et al.  BRASSINOSTEROIDS: Essential Regulators of Plant Growth and Development. , 1998, Annual review of plant physiology and plant molecular biology.

[9]  E. Rassart,et al.  Cloning, characterization, and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. , 1992, Plant physiology.

[10]  Søren Brunak,et al.  A Neural Network Method for Identification of Prokaryotic and Eukaryotic Signal Peptides and Prediction of their Cleavage Sites , 1997, Int. J. Neural Syst..

[11]  A. D. Hieber,et al.  Xanthophyll Cycle Enzymes Are Members of the Lipocalin Family, the First Identified from Plants* , 1998, The Journal of Biological Chemistry.

[12]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[13]  R. Bishop,et al.  The bacterial lipocalins. , 2000, Biochimica et biophysica acta.

[14]  J. Weiner,et al.  Stationary Phase Expression of a Novel Escherichia coli Outer Membrane Lipoprotein and Its Relationship with Mammalian Apolipoprotein D , 1995, The Journal of Biological Chemistry.

[15]  Yoshihiro Ugawa,et al.  Plant cis-acting regulatory DNA elements (PLACE) database: 1999 , 1999, Nucleic Acids Res..

[16]  K. Nakai,et al.  PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. , 1999, Trends in biochemical sciences.

[17]  Frans,et al.  Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones , 1994, Plant physiology.

[18]  D. Sanchez,et al.  Lazarillo, a new GPI-linked surface lipocalin, is restricted to a subset of neurons in the grasshopper embryo. , 1995, Development.

[19]  E. Schleiff,et al.  Lipid composition of outer leaflet of chloroplast outer envelope determines topology of OEP7. , 2001, Molecular biology of the cell.

[20]  M C Peitsch,et al.  ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling. , 1996, Biochemical Society transactions.