Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase

To avoid the use of compounds that burden the environment, a solvent-free enzymatic reaction was the focus of this study. Investigated were the catalytic activities and kinetics of lipases that were pretreated with carboxylic acids for the solvent-free esterification of propionic acid with isoamyl alcohol. The enhancements of the esterification yields and rates by the bio-imprinting effects of carboxylic acids were observed. We found no inhibition of isoamyl alcohol on the solvent-free enzymatic esterification, and obtained a large imprinting effect under a largely excessive amount of isoamyl alcohol to propionic acid. From the kinetic analysis, the imprinting of lipases mainly enhanced the catalytic reaction rate constant rather than the affinity between lipase and propionic acid compared with untreated lipase. The bio-imprinting treatment of lipase is found to be very effective for the yield and kinetics in solvent-free esterification.

[1]  M. Matsumoto,et al.  Enhanced activities and thermostability of lipase pretreated with carboxylic and perflurocarboxylic acids in transesterification , 2018, Journal of Chemical Technology & Biotechnology.

[2]  V. Rathod,et al.  Optimization of enzymatic synthesis of ethyl hexanoate in a solvent free system using response surface methodology (RSM) , 2018 .

[3]  T. Friščić,et al.  Solvent-Free Enzyme Activity: Quick, High-Yielding Mechanoenzymatic Hydrolysis of Cellulose into Glucose. , 2018, Angewandte Chemie.

[4]  B. Kiss,et al.  Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts , 2016 .

[5]  Yanjun Jiang,et al.  Lipase Immobilization through the Combination of Bioimprinting and Cross-Linked Protein-Coated Microcrystal Technology for Biodiesel Production , 2016 .

[6]  Y. Chisti,et al.  Transesterification of primary and secondary alcohols using Pseudomonas aeruginosa lipase. , 2008, Bioresource technology.

[7]  E. Yılmaz Improving the application of microbial lipase by bio-imprinting at substrate-interfaces , 2002 .

[8]  Koji Kida,et al.  Enhanced activities of lipase pretreated with organic solvents , 2001 .

[9]  Koji Kida,et al.  Effects of polyols and organic solvents on thermostability of lipase , 1997 .

[10]  H. González-Navarro,et al.  Improving lipase activity in solvent-free media by interfacial activation-based molecular bioimprinting , 1997 .

[11]  Mercedes Martínez,et al.  Kinetic modelling of esterification reactions catalysed by immobilized lipases , 1996 .

[12]  J. Dordick,et al.  Organic solvents strip water off enzymes , 1992, Biotechnology and bioengineering.

[13]  Xianfu Lin,et al.  Effect of Additives on the Selectivity and Reactivity of Enzymes. , 2017, Chemical record.

[14]  M. N. Gupta Non-Aqueous Enzymology: Issues and Persspectives , 2000 .