Microcystis aeruginosa lipids as feedstock for biodiesel synthesis by enzymatic route

Abstract The cyanobacterium Microcystis aeruginosa strain NPCD-1, isolated from sewage treatment plant and characterized as a non-microcystin producer by mass spectrometry and molecular analysis, was found to be a source of lipid when cultivated in ASM-1 medium at 25 °C under constant white fluorescent illumination (109 μmol photon m −2  s −1 ). In these conditions, biomass productivity of 46.92 ± 3.84 mg L −1  day −1 and lipid content of 28.10 ± 1.47% were obtained. Quantitative analysis of fatty acid methyl esters demonstrated high concentration of saturated fatty acids (50%), palmitic (24.34%) and lauric (13.21%) acids being the major components. The remaining 50% constituting unsaturated fatty acids showed higher concentrations of oleic (26.88%) and linoleic (12.53%) acids. The feasibility to produce biodiesel from this cyanobacterial lipid was demonstrated by running enzymatic transesterification reactions catalyzed by Novozym ® 435 and using palm oil as feedstock control. Batch experiments were carried out using tert -butanol and iso-octane as solvent. Results showed similarity on the main ethyl esters formed for both feedstocks. The highest ethyl ester concentration was related to palmitate and oleate esters followed by laurate and linoleate esters. However, both reaction rates and ester yields were dependent on the solvent tested. Total ethyl ester concentrations varied in the range of 44.24–67.84 wt%, corresponding to ester yields from 80 to 100%. Iso-octane provided better solubility and miscibility, with ester yield of 98.10% obtained at 48 h for reaction using the cyanobacterium lipid, while full conversion was achieved in 12 h for reaction carried out with palm oil. These results demonstrated that cyanobacterial lipids from M. aeruginosa NPCD-1 have interesting properties for biofuel production.

[1]  J. Trevors,et al.  Miniprep DNA isolation from unicellular and filamentous cyanobacteria. , 2000, Journal of microbiological methods.

[2]  E. Dittmann,et al.  Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806: an integrated peptide-polyketide synthetase system. , 2000, Chemistry & biology.

[3]  W. Carmichael,et al.  Human intoxication by microcystins during renal dialysis treatment in Caruaru-Brazil. , 2002, Toxicology.

[4]  T. Franco,et al.  Microalgae as feedstock for biodiesel production: Carbon dioxide sequestration, lipid production and biofuel quality , 2010 .

[5]  Y. Chisti.  Biodiesel from microalgae beats bioethanol. , 2008, Trends in biotechnology.

[6]  R. Sakai,et al.  Structures of three new cyclic heptapeptide hepatotoxins produced by the cyanobacterium (blue-green alga) nostoc sp. strain 1521 , 1990 .

[7]  Jorge Alberto Vieira Costa,et al.  The role of biochemical engineering in the production of biofuels from microalgae. , 2011, Bioresource technology.

[8]  Heizir F. de Castro,et al.  Biodiesel Synthesis by Enzymatic Transesterification of Palm Oil with Ethanol Using Lipases from Several Sources Immobilized on Silica-PVA Composite , 2007 .

[9]  Palligarnai T. Vasudevan,et al.  Effect of Organic Solvents on Enzyme-Catalyzed Synthesis of Biodiesel , 2009 .

[10]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[11]  C. Kenyon.  Fatty Acid Composition of Unicellular Strains of Blue-Green Algae , 1972, Journal of bacteriology.

[12]  S. Azevedo,et al.  The first evidence of paralytic shellfish toxins in the fresh water cyanobacterium Cylindrospermopsis raciborskii, isolated from Brazil. , 1999, Toxicon : official journal of the International Society on Toxinology.

[13]  B. Neilan,et al.  Detection of Toxigenicity by a Probe for the Microcystin Synthetase A Gene (mcyA) of the Cyanobacterial Genus Microcystis: Comparison of Toxicities with 16S rRNA and Phycocyanin Operon (Phycocyanin Intergenic Spacer) Phylogenies , 2001, Applied and Environmental Microbiology.

[14]  Caroline Souza Pamplona Silva,et al.  Non-ribosomal peptides produced by Brazilian cyanobacterial isolates with antimicrobial activity. , 2011, Microbiological research.

[15]  Frank D. Gunstone,et al.  The Lipid handbook , 1994 .

[16]  Xuewu Zhang,et al.  Biodiesel Production by Microalgal Biotechnology , 2018, Renewable Energy.

[17]  Ana Cristina Oliveira,et al.  Microalgae as a raw material for biofuels production , 2009, Journal of Industrial Microbiology & Biotechnology.

[18]  M. Welker,et al.  Cyanobacterial peptides - nature's own combinatorial biosynthesis. , 2006, FEMS microbiology reviews.

[19]  J. Vaitomaa,et al.  Phylogenetic evidence for the early evolution of microcystin synthesis. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Kati Laakso,et al.  Recurrent adenylation domain replacement in the microcystin synthetase gene cluster , 2007, BMC Evolutionary Biology.

[21]  S. Harrison,et al.  Lipid productivity as a key characteristic for choosing algal species for biodiesel production , 2009, Journal of Applied Phycology.

[22]  Paul A Haynes,et al.  Liver Membrane Proteome Glycosylation Changes in Mice Bearing an Extra-hepatic Tumor , 2010, Molecular & Cellular Proteomics.

[23]  M. B. A. Castro,et al.  Síntese de padrões cromatográficos e estabelecimento de método para dosagem da composição de ésteres de ácidos graxos presentes no biodiesel a partir do óleo de babaçu , 2008 .

[24]  W. Carmichael,et al.  Sublethal exposure from microcystins to renal insufficiency patients in Rio de Janeiro, Brazil , 2006, Environmental toxicology.

[25]  K. Rinehart,et al.  Structure and biosynthesis of toxins from blue-green algae (cyanobacteria) , 1994, Journal of Applied Phycology.

[26]  J. Vaitomaa,et al.  Effect of Nitrogen and Phosphorus on Growth of Toxic and Nontoxic Microcystis Strains and on Intracellular Microcystin Concentrations , 2002, Microbial Ecology.

[27]  F. Marner,et al.  Seaweed dermatitis: structure of lyngbyatoxin A. , 1979, Science.

[28]  K. Krisnangkura.  A simple method for estimation of cetane index of vegetable oil methyl esters , 1986 .

[29]  P. Cook,et al.  Ingested mineral fibers: elimination in human urine. , 1979, Science.

[30]  G. Dönmez,et al.  Microbial oil production from thermophile cyanobacteria for biodiesel production , 2011 .

[31]  A. Ahmad,et al.  Microalgae as a sustainable energy source for biodiesel production: A review , 2011 .

[32]  H. Oh,et al.  Microcystin Production by Microcystis aeruginosa in a Phosphorus-Limited Chemostat , 2000, Applied and Environmental Microbiology.

[33]  S. Oishi,et al.  Simultaneous production of homoanatoxin-a, anatoxin-a, and a new non-toxic 4-hydroxyhomoanatoxin-a by the cyanobacterium Raphidiopsis mediterranea Skuja. , 2003, Toxicon : official journal of the International Society on Toxinology.

[34]  B. Ferrari,et al.  Comparative Protein Expression in Different Strains of the Bloom-forming Cyanobacterium Microcystis aeruginosa* , 2011, Molecular & Cellular Proteomics.

[35]  Philip R. Cohen,et al.  Cyanobacterial microcystin‐LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants , 1990, FEBS letters.

[36]  W. Carmichael,et al.  Human Fatalities from Cyanobacteria: Chemical and Biological Evidence for Cyanotoxins , 2001 .

[37]  M. Demirbas,et al.  IMPORTANCE OF ALGAE OIL AS A SOURCE OF BIODIESEL , 2011 .

[38]  S. Oishi,et al.  Isolation and identification of homoanatoxin-a from a toxic strain of the cyanobacterium Raphidiopsis mediterranea Skuja isolated from Lake Biwa, Japan , 2003 .

[39]  L. Rodolfi,et al.  Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor , 2009, Biotechnology and bioengineering.

[40]  S. Mandal,et al.  Microalga Scenedesmus obliquus as a potential source for biodiesel production , 2009, Applied Microbiology and Biotechnology.