Signal Transduction during Platelet‐Activating Factor‐Induced Lymphocyte Binding to Endothelial Cells

Platelet‐activating factor (PAF) is a lipid mediator of inflammation. PAF pretreatment of cultured endothelial cells leads to an increase in lymphocyte binding. We have analysed the intracellular signal transduction during this PAF‐induced effect. The protein kinase C activator, phorbol 12‐myristate, 13‐acetate, mimicked PAF in the binding assay. Concomitantly, the protein kinase C (PKC) inhibitor H7 down‐regulated the PAF‐induced binding to nearly control level. Also dibutyryl‐cAMP treatment of endothelial cells increased lymphocyte binding, but the protein kinase A inhibitor HA1004 did not alter the PAF‐induced binding. Furthermore, PAF did not increase the level of cytosolic cAMP in the endothelial cells. Other second messengers. cGMPandCa2+, had no effect on lymphocyte binding. These fmdings suggest that protein kinase C, but no other signal transduction pathway, is essential in the PAF‐induced lymphocyte binding to endothelial cells.

[1]  P. Mattila,et al.  Interleukin 1‐induced lymphocyte binding to endothelial cells. Role of cAMP as a second messenger , 1990, European journal of immunology.

[2]  J. Turunen,et al.  Lymphocyte Binding to and Penetration through Vascular Endothelium is Stimulated by Platelet‐Activating Factor , 1989, Scandinavian journal of immunology.

[3]  P. Mattila,et al.  Antigen expression in different parenchymal cell types of rat kidney and heart. , 1989, Kidney international.

[4]  A. Hamann,et al.  Mechanisms and regulation of lymphocyte migration. , 1989, Immunology today.

[5]  J. Gay,et al.  Enhancement of phorbol ester‐induced protein kinase activity in human neutrophils by platelet‐activating factor , 1988, Journal of cellular physiology.

[6]  F. Breviario,et al.  IL-1-induced adhesion of polymorphonuclear leukocytes to cultured human endothelial cells. Role of platelet-activating factor. , 1988, Journal of immunology.

[7]  P. Mattila,et al.  Leukotriene B4 increases the lymphocyte binding to endothelial cells , 1988, FEBS letters.

[8]  J. Kuo,et al.  Regulation of protein kinase C by lysophospholipids. Potential role in signal transduction. , 1988, The Journal of biological chemistry.

[9]  J. Gay,et al.  Platelet-activating factor induces protein kinase activity in the particulate fraction of human neutrophils. , 1988, Blood.

[10]  Y. Saegusa,et al.  Stimulation of endothelial cell binding of lymphocytes by tumor necrosis factor. , 1987, Journal of immunology.

[11]  U. Ryan,et al.  Platelet‐Activating Factor Effects on Bovine Pulmonary Artery Endothelial Cells , 1987, Circulation research.

[12]  G. Zimmerman,et al.  Production of platelet-activating factor by stimulated human polymorphonuclear leukocytes. Correlation of synthesis with release, functional events, and leukotriene B4 metabolism. , 1987, Journal of immunology.

[13]  J. O'flaherty,et al.  Arachidonate metabolites, platelet-activating factor, and the mobilization of protein kinase C in human polymorphonuclear neutrophils. , 1987, Journal of immunology.

[14]  P. Braquet,et al.  Platelet-activating factor and cellular immune responses. , 1987, Immunology today.

[15]  C. Parker.  Lipid mediators produced through the lipoxygenase pathway. , 1987, Annual review of immunology.

[16]  J. Woodruff,et al.  Specific cell-adhesion mechanisms determining migration pathways of recirculating lymphocytes. , 1987, Annual review of immunology.

[17]  E. Butcher,et al.  Homing Receptors and the Control of Lymphocyte Migration , 1986, Immunological reviews.

[18]  N. Minato,et al.  Mechanisms of lymphocyte adhesion to human vascular endothelial cells in culture. T lymphocyte adhesion to endothelial cells through endothelial HLA-DR antigens induced by gamma interferon. , 1986, The Journal of clinical investigation.

[19]  D. Haskard,et al.  Interleukin 1 increases the binding of human B and T lymphocytes to endothelial cell monolayers. , 1986, Journal of immunology.

[20]  D. Macintyre,et al.  Regulation of platelet cytosolic free calcium by cyclic nucleotides and protein kinase C , 1985, FEBS Letters.

[21]  E. Corey,et al.  Leukotriene B4 action on endothelium mediates augmented neutrophil/endothelial adhesion. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[22]  R. Renkonen,et al.  Lymphoid cell subclasses in rejecting renal allograft in the rat. , 1983, Cellular immunology.

[23]  Y. Nishizuka,et al.  Phosphorylation of calf thymus H1 histone by calcium-activated, phospholipid-dependent protein kinase. , 1980, Biochemical and biophysical research communications.

[24]  E. J. Knight,et al.  The route of re-circulation of lymphocytes in the rat , 1964, Proceedings of the Royal Society of London. Series B. Biological Sciences.