A single-layer structured microbial sensor for fast detection of biochemical oxygen demand

Abstract In this paper, a novel biochemical oxygen demand (BOD) sensor based on a single layer of immobilized microorganisms on electrode for fast detection is investigated. Instead of the previous two-layer structure of oxygen permselective membrane and microorganism membrane, a single layer structure using electrodes as the support for microorganism immobilization has been studied. Amino functional groups on the surface of microorganisms have been bonded with carboxyl modified electrodes. Without extra supports for immobilization, mass transfer resistances are reduced, which facilitates fast current response. B acillus subtilis cells with high biodegradation ability are obtained after 12 h cultivation. The immobilization process has been investigated by CV and EIS. B. subtilis cultivation time, response time and linear range have been optimized. The proposed sensor can reach equilibrium in 5 min which has the advantage for real-time BOD determination in the future. The linear range is 5–30 mg/l with correlation coefficient of 0.978 and limit of detection of 1.65 mg/l.

[1]  Bienzyme self-assembled monolayer on gold electrode: An amperometric biosensor for carbaryl determination , 2013 .

[2]  I Karube,et al.  Optical fiber biosensor for the determination of low biochemical oxygen demand. , 2000, Biosensors & bioelectronics.

[3]  Xin-qiang Liang,et al.  Optimization of FDA–PI method using flow cytometry to measure metabolic activity of the cyanobacteria, Microcystis aeruginosa , 2011 .

[4]  Yun-guo Liu,et al.  Biosorption of copper(II) from aqueous solution by Bacillus subtilis cells immobilized into chitosan beads , 2013 .

[5]  A. Boukabache,et al.  Methods for assessing biochemical oxygen demand (BOD): a review. , 2014, Water research.

[6]  M. Izumi,et al.  Effects of self-assembled monolayers on amperometric glucose biosensors based on an organic–inorganic hybrid system , 2012 .

[7]  Isao Karube,et al.  Fabrication of oxygen electrode arrays and their incorporation into sensors for measuring biochemical oxygen demand , 1997 .

[8]  Ling Liu,et al.  Organic-inorganic hybrid material for the cells immobilization: long-term viability mechanism and application in BOD sensors. , 2009, Biosensors & bioelectronics.

[9]  A. N. Reshetilov,et al.  Microbial biosensors for detection of biological oxygen demand (a Review) , 2011, Applied Biochemistry and Microbiology.

[10]  Isao Karube,et al.  A chemiluminescence biochemical oxygen demand measuring method. , 2007, Analytica chimica acta.

[11]  N. Pasco,et al.  The use of microorganisms with broad range substrate utilisation for the ferricyanide-mediated rapid determination of biochemical oxygen demand. , 2001, Talanta.

[12]  Xiaohong Li,et al.  The influence of gold nanoparticle modified electrode on the structure of mercaptopropionic acid self-assembly monolayer , 2005 .

[13]  Guo-Li Shen,et al.  Inhibitive determination of mercury ion using a renewable urea biosensor based on self-assembled gold nanoparticles , 2006 .

[14]  M. Y. Arica,et al.  Removal of heavy mercury(II), cadmium(II) and zinc(II) metal ions by live and heat inactivated Lentinus edodes pellets , 2008 .

[15]  Maria D. L. Oliveira,et al.  Electrochemical immunosensor for dengue virus serotypes based on 4-mercaptobenzoic acid modified gold nanoparticles on self-assembled cysteine monolayers , 2015 .

[16]  Isao Karube,et al.  Absorption-based highly sensitive and reproducible biochemical oxygen demand measurement method for seawater using salt-tolerant yeast Saccharomyces cerevisiae ARIF KD-003. , 2008, Analytica chimica acta.

[17]  Ling Liu,et al.  Immobilized multi-species based biosensor for rapid biochemical oxygen demand measurement. , 2011, Biosensors & bioelectronics.

[18]  H. Luo,et al.  A reusable and label-free supersandwich biosensor for sensitive DNA detection by immobilizing target-triggered DNA concatamers on ternary self-assembled monolayer , 2016 .

[19]  Z. Qian,et al.  Dead Bacillus subtilis cells for sensing biochemical oxygen demand of waters and wastewaters , 1997 .

[20]  Ling Liu,et al.  A new mediator method for BOD measurement under non-deaerated condition. , 2010, Talanta.

[21]  Shaojun Dong,et al.  Co-immobilized microbial biosensor for BOD estimation based on sol-gel derived composite material. , 2003, Biosensors & bioelectronics.

[22]  R. Renneberg,et al.  Designing an amperometric thick-film microbial BOD sensor. , 2000, Biosensors & bioelectronics.

[23]  G. Palleschi,et al.  Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection , 2013 .

[24]  I. Karube,et al.  Monitoring of the composting process using a mediator-type biochemical oxygen demand sensor , 2001 .

[25]  Zuoming Zhou,et al.  Enhanced NOx removal from flue gas by an integrated process of chemical absorption coupled with two-stage biological reduction using immobilized microorganisms , 2013 .

[26]  Y. Wong,et al.  Removal and biodegradation of nonylphenol by immobilized Chlorella vulgaris. , 2011, Bioresource technology.

[27]  Piero Cosseddu,et al.  Electrochemical characterization of self assembled monolayers on flexible electrodes , 2012 .

[28]  Chih-Hung Wu,et al.  Evaluation of different cell-immobilization strategies for simultaneous distillery wastewater treatment and electricity generation in microbial fuel cells , 2015 .

[29]  K. Bandyopadhyay,et al.  Formation of a Self-Assembled Monolayer of Diphenyl Diselenide on Polycrystalline Gold , 1998 .

[30]  R. K. Shervedani,et al.  Functionalization of gold mercaptopropionic acid self-assembled monolayer with 5-amino-1,10-phenanthroline: Interaction with iron(II) and application for selective recognition of guanine , 2015 .

[31]  P. Teasdale,et al.  A ferricyanide-mediated activated sludge bioassay for fast determination of the biochemical oxygen demand of wastewaters. , 2010, Water research.

[32]  A. Ulman,et al.  Formation and Structure of Self-Assembled Monolayers. , 1996, Chemical reviews.

[33]  Yeyao Wang,et al.  An innovative reactor-type biosensor for BOD rapid measurement. , 2010, Biosensors & bioelectronics.

[34]  Kenji Yokoyama,et al.  A rapid BOD sensing system using luminescent recombinants of Escherichia coli. , 2003, Biosensors & bioelectronics.

[35]  R. K. Shervedani,et al.  Comparative Electrochemical Behavior of Proteins; Cytochrome c, Agaricus Bisporus Laccase, and Glucose Oxidase, Immobilized onto Gold-Thiol Self-Assembled Monolayer via Electrostatic, Covalent, and Covalent Coordinate Bond Methods , 2016 .

[36]  Isao Karube,et al.  A new BOD estimation method employing a double-mediator system by ferricyanide and menadione using the eukaryote Saccharomyces cerevisiae. , 2007, Talanta.

[37]  V. A. Alferov,et al.  BOD biosensor based on the yeast Debaryomyces hansenii immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone. , 2013, Enzyme and microbial technology.

[38]  Ashok Kumar,et al.  Quick and reliable estimation of BOD load of beverage industrial wastewater by developing BOD biosensor , 2008 .

[39]  Shaojun Dong,et al.  An optical biosensor for multi-sample determination of biochemical oxygen demand (BOD) , 2005 .

[40]  Bansi D Malhotra,et al.  Recent advances in self-assembled monolayers based biomolecular electronic devices. , 2009, Biosensors & bioelectronics.

[41]  V. N. Paunov,et al.  Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems. , 2010, Biosensors & bioelectronics.

[42]  I Karube,et al.  A mediator-type biosensor as a new approach to biochemical oxygen demand estimation. , 2000, The Analyst.

[43]  I Karube,et al.  Microbial electrode BOD sensors , 1977, Biotechnology and bioengineering.

[44]  Yasuaki Einaga,et al.  Development of a biochemical oxygen demand sensor using gold-modified boron doped diamond electrodes. , 2012, Analytical chemistry.

[45]  I. Karube,et al.  A SIMPLE, MULTIPLE SIMULTANEOUS SPECTROPHOTOMETRIC METHOD FOR BOD DETERMINATION USING DCIP AS THE REDOX COLOR INDICATOR , 2002 .