The rare earth metal doping in metal oxides semiconductor taken into account as an efficient approach for enhancing structural activity due to its benefit in adjusting optical properties and easing charge carrier recombination. The crystal structure, optical, magnetic, and antibacterial properties of the samples were extensively analyzed as a function of Ce-substituted NiCr<sub>2</sub>O<sub>4</sub> material. As the Ce concentration increases, the size of the nanoparticles decreases from ∼33 to 22nnm. The undoped NiCr<sub>2</sub>O<sub>4</sub> and Ce doped NiCr<sub>2</sub>O<sub>4</sub> materials improves the local disruption at both the octahedral and tetrahedral sub lattices. In the present work, the results indicate that the substitution of Ce ion into nickel chromite particles, which strongly affects the magnetic, optical, crystal structure, particle diameter and microstructure, and also enhances antibacterial properties.