Adhesion of Campylobacter jejuni Is Increased in Association with Foodborne Bacteria

The aim of this study was to evaluate Campylobacter jejuni NTCT 11168 adhesion to abiotic and biotic surfaces when grown in co-culture with Escherichia coli ATCC 11229 and/or Listeria monocytogenes 4b. Adhesion of C. jejuni to polystyrene and to Caco-2 cells and Acanthamoeba castellanii was lower for at least 3 log CFU/mL compared to E. coli and L. monocytogenes. Electron micrographs of ultrathin sections revealed interactions of C. jejuni with host cells. In co-culture with E. coli and L. monocytogenes, adhesion of C. jejuni to all tested surfaces was significantly increased for more than 1 log CFU/mL. There was 10% higher aggregation for C. jejuni than for other pathogens, and high co-aggregation of co-cultures of C. jejuni with E. coli and L. monocytogenes. These data show that C. jejuni in co-cultures with E. coli and L. monocytogenes present significantly higher risk than C. jejuni as mono-cultures, which need to be taken into account in risk evaluation. C. jejuni adhesion is a prerequisite for their colonization, biofilm formation, and further contamination of the environment. C. jejuni survival under adverse conditions as a factor in their pathogenicity and depends on their adhesion to different surfaces, not only as individual strains, but also in co-cultures with other bacteria like E. coli and L. monocytogenes.

[1]  W. Messens,et al.  The European Union One Health 2018 Zoonoses Report , 2019, EFSA journal. European Food Safety Authority.

[2]  K. Nagy,et al.  The European Union summary report on trends and sources of zoonoses, zoonotic agents and food‐borne outbreaks in 2017 , 2018, EFSA journal. European Food Safety Authority.

[3]  Wei Chen,et al.  The Effect of Co-infection of Food-Borne Pathogenic Bacteria on the Progression of Campylobacter jejuni Infection in Mice , 2018, Front. Microbiol..

[4]  A. Klančnik,et al.  Reduced contamination and infection via inhibition of adhesion of foodborne bacteria to abiotic polystyrene and biotic amoeba surfaces , 2018 .

[5]  A. V. Karlyshev,et al.  CmeABC Multidrug Efflux Pump Contributes to Antibiotic Resistance and Promotes Campylobacter jejuni Survival and Multiplication in Acanthamoeba polyphaga , 2017, Applied and Environmental Microbiology.

[6]  P. Ammini,et al.  The Food Production Environment and the Development of Antimicrobial Resistance in Human Pathogens of Animal Origin , 2017, Microorganisms.

[7]  K. Trošt,et al.  Anti‐Campylobacter activity of resveratrol and an extract from waste Pinot noir grape skins and seeds, and resistance of Camp. jejuni planktonic and biofilm cells, mediated via the CmeABC efflux pump , 2017, Journal of applied microbiology.

[8]  G. Koudelka,et al.  Determinants that govern the recognition and uptake of Escherichia coli O157 : H7 by Acanthamoeba castellanii , 2016, Cellular microbiology.

[9]  A. Nagy,et al.  Ralstonia insidiosa induces cell aggregation of Listeria monocytogenes , 2016 .

[10]  D. Shelton,et al.  Ralstonia insidiosa serves as bridges in biofilm formation by foodborne pathogens Listeria monocytogenes, Salmonella enterica, and Enterohemorrhagic Escherichia coli , 2016 .

[11]  D. Hendrixson,et al.  Campylobacter jejuni CsrA Regulates Metabolic and Virulence Associated Proteins and Is Necessary for Mouse Colonization , 2016, PloS one.

[12]  A. Klančnik,et al.  Alpinia katsumadai Extracts Inhibit Adhesion and Invasion of Campylobacter jejuni in Animal and Human Foetal Small Intestine Cell Lines , 2015, Phytotherapy research : PTR.

[13]  Agapi I. Doulgeraki,et al.  Intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens , 2015, Front. Microbiol..

[14]  Si Ming Man,et al.  Global Epidemiology of Campylobacter Infection , 2015, Clinical Microbiology Reviews.

[15]  I. Pavord,et al.  Blood eosinophil counts, exacerbations, and response to the addition of inhaled fluticasone furoate to vilanterol in patients with chronic obstructive pulmonary disease: a secondary analysis of data from two parallel randomised controlled trials. , 2015, The Lancet. Respiratory medicine.

[16]  D. Bolton.  Campylobacter virulence and survival factors. , 2015, Food microbiology.

[17]  P. Jamnik,et al.  Stress Response and Virulence of Heat-Stressed Campylobacter jejuni , 2014, Microbes and environments.

[18]  S. L. Svensson,et al.  Flagella-Mediated Adhesion and Extracellular DNA Release Contribute to Biofilm Formation and Stress Tolerance of Campylobacter jejuni , 2014, PloS one.

[19]  Sui-Mae Lee,et al.  Does Campylobacter jejuni Form Biofilms in Food-Related Environments? , 2014, Applied and Environmental Microbiology.

[20]  A. V. Karlyshev,et al.  A negative effect of Campylobacter capsule on bacterial interaction with an analogue of a host cell receptor , 2014, BMC Microbiology.

[21]  Jarmila Pazlarova,et al.  Influence of food processing environments on structure initiation of static biofilm of Listeria monocytogenes , 2014 .

[22]  P. Ellström,et al.  Campylobacter jejuni Actively Invades the Amoeba Acanthamoeba polyphaga and Survives within Non Digestive Vacuoles , 2013, PloS one.

[23]  S. Backert,et al.  Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni. , 2013, Journal of microbiological methods.

[24]  M. Loessner,et al.  Acanthamoeba feature a unique backpacking strategy to trap and feed on Listeria monocytogenes and other motile bacteria. , 2013, Environmental microbiology.

[25]  J. Bolla,et al.  Enhanced Adhesion of Campylobacter jejuni to Abiotic Surfaces Is Mediated by Membrane Proteins in Oxygen-Enriched Conditions , 2012, PloS one.

[26]  C. Szymanski,et al.  How a sugary bug gets through the day , 2012, Gut microbes.

[27]  P. Ellström,et al.  Amoebae and algae can prolong the survival of Campylobacter species in co-culture. , 2010, Experimental parasitology.

[28]  B. Pearson,et al.  Biofilm Formation by Campylobacter jejuni Is Increased under Aerobic Conditions , 2010, Applied and Environmental Microbiology.

[29]  K. Braeckmans,et al.  Influence of temperature, oxygen and bacterial strain identity on the association of Campylobacter jejuni with Acanthamoeba castellanii. , 2009, FEMS microbiology ecology.

[30]  A. Akya,et al.  Viability of Listeria monocytogenes in co-culture with Acanthamoeba spp. , 2009, FEMS microbiology ecology.

[31]  P. Jamnik,et al.  Stress response and pathogenic potential of Campylobacter jejuni cells exposed to starvation. , 2009, Research in microbiology.

[32]  A. Klančnik,et al.  Survival of stress exposed Campylobacter jejuni in the murine macrophage J774 cell line. , 2009, International journal of food microbiology.

[33]  Sheriase Q Sanders,et al.  Temperature and nutrient effects on Campylobacter jejuni attachment on multispecies biofilms on stainless steel. , 2008, Journal of food protection.

[34]  A. Klančnik,et al.  Environmental stress factors affecting survival and virulence of Campylobacter jejuni. , 2007, Microbial pathogenesis.

[35]  J. Frece,et al.  Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92 , 2003, Journal of applied microbiology.

[36]  N. High,et al.  Bacterial coaggregation: an integral process in the development of multi-species biofilms. , 2003, Trends in microbiology.

[37]  M. R. Brown,et al.  Unexplored reservoirs of pathogenic bacteria: protozoa and biofilms. , 1999, Trends in microbiology.