High-throughput analysis of tissue-based biomarkers in digital pathology

By simultaneously processing a large number of tissue samples, the tissue microarray (TMA) technology allows standardized screening of protein expression using immunohistochemistry thereby providing a very efficient way for tissue-based biomarker analysis. Nowadays, whole slide imaging is becoming standard in digital pathology and enables image sharing, archiving and also processing. In this paper, we present methods for processing TMA images in order to correctly identify the numerous tissue samples and to register images from consecutive TMA sections.

[1]  Stephen M Hewitt,et al.  Validation of proteomic‐based discovery with tissue microarrays , 2008, Proteomics. Clinical applications.

[2]  C. Loos.  Multiple Immunoenzyme Staining: Methods and Visualizations for the Observation With Spectral Imaging , 2008 .

[3]  Yves-Rémi Van Eycke,et al.  Registration of whole immunohistochemical slide images: an efficient way to characterize biomarker colocalization , 2015, J. Am. Medical Informatics Assoc..

[4]  C. Womack,et al.  A matrix approach to guide IHC‐based tissue biomarker development in oncology drug discovery , 2014, The Journal of pathology.

[5]  Isabelle Salmon,et al.  Whole slide imaging and analysis for biomarker evaluation in digital pathology , 2014 .

[6]  T Krenacs,et al.  Multiple antigen immunostaining procedures. , 1999, Methods in molecular biology.

[7]  F. Cordelières,et al.  A guided tour into subcellular colocalization analysis in light microscopy , 2006, Journal of microscopy.

[8]  M. Basik,et al.  Tissue microarrays: emerging standard for biomarker validation. , 2008, Current opinion in biotechnology.

[9]  Todd H. Stokes,et al.  Pathology imaging informatics for quantitative analysis of whole-slide images , 2013, Journal of the American Medical Informatics Association : JAMIA.

[10]  Kai Stühler,et al.  High‐performance Proteomics as a Tool in Biomarker Discovery , 2007, Proteomics.

[11]  M. Raffeld,et al.  Multiple antigen immunostaining procedures. , 2010, Methods in molecular biology.

[12]  A. Ruifrok,et al.  Quantification of histochemical staining by color deconvolution. , 2001, Analytical and quantitative cytology and histology.