Diel rhythms in shallow Mediterranean rocky-reef fishes: a chronobiological approach with the help of trained volunteers

Behavioural rhythms in marine species have been mostly investigated in laboratory organisms and their expression within the animals' natural environments remains largely unknown. Here, we studied diel (i.e. 24-hours-based) and intra-diel (i.e. 12-hours-based) rhythmic variations in the abundance of seven shallow rocky-reef fish species, namely Coris julis, Epinephelus marginatus, Sarpa salpa, Serranus cabrilla, Serranus scriba, Sparisoma cretense and Thalassoma pavo, along the rocky shores of Linosa Island (Mediterranean Sea). Data were visually collected by trained volunteers along fixed transects at 3-hourly intervals throughout six consecutive 24-hours periods. Density estimates can vary greatly between consecutive days and during 24-hours periods according not only to the major day–night changeover but also to minor intra-diel variations at the daylight hours. In the case of T. pavo, C. julis, S. cabrilla and S. salpa waveform analyses showed midday troughs in abundance within the 24-hours period but significant variation within the hours of daylight was highlighted only for T. pavo. Although results were not conclusive at the intra-dial level, the employment of volunteers represented a valuable tool for chronobiology, suitable to improve our understanding of fish behaviour in natural systems.

[1]  E. Hobson Diurnal-Nocturnal Activity of Some Inshore Fishes in the Gulf of California , 1965 .

[2]  A. W. Ebeling,et al.  DAY VERSUS NIGHT ACTIVITY OF REEF FISHES IN A KELP FOREST OFF SANTA BARBARA, CALIFORNIA , 1976 .

[3]  J. Aschoff Freerunning and Entrained Circadian Rhythms , 1981 .

[4]  M. Harmelin-Vivien,et al.  Fish fauna of French Mediterranean Posidonia oceanica seagrass meadows. II: feeding habits , 1983 .

[5]  F. Blanc,et al.  Evaluation visuelle des peuplements et populations de poissons : méthodes et problèmes , 1985, Revue d'Écologie (La Terre et La Vie).

[6]  G. Helfman Fish Behaviour by Day, Night and Twilight , 1986 .

[7]  M. Smith Effects of observer swimming speed on sample counts of temperate rocky reef fish assemblages , 1988 .

[8]  K. N’da,et al.  Sexual cycle and seasonal changes in the ovary of the red mullet, Mullus surmuletus, from the southern coast of Brittany , 1993 .

[9]  K. R. Clarke,et al.  Non‐parametric multivariate analyses of changes in community structure , 1993 .

[10]  G. Copp,et al.  Do small riverine fish move inshore at night , 1993 .

[11]  J. Greenwood Trust the wildlife volunteers , 1994, Nature.

[12]  E. V. Berghe,et al.  Diurnal Abundance Patterns of Mediterranean Fishes Assessed on Fixed Transects by Scuba Divers , 1995 .

[13]  B. Mapstone,et al.  Observer effects and training in underwater visual surveys of reef fishes , 1997 .

[14]  J. Palmer The clocks controlling the tide-associated rhythms of intertidal animals. , 2000, BioEssays : news and reviews in molecular, cellular and developmental biology.

[15]  M Schimmel,et al.  Emphasizing Difficulties in the Detection of Rhythms with Lomb-Scargle Periodograms , 2001, Biological rhythm research.

[16]  Marti J. Anderson,et al.  A new method for non-parametric multivariate analysis of variance in ecology , 2001 .

[17]  M. Harmelin-Vivien,et al.  Spatial and temporal variations of fish assemblages in a shallow Mediterranean soft-bottom area (Gulf of Fos, France) , 2001 .

[18]  S. M. Evans,et al.  Volunteers assess marine biodiversity. , 2001, Biologist.

[19]  Belinda G. Curley,et al.  Spatial and habitat-related patterns of temperate reef fish assemblages: implications for the design of Marine Protected Areas , 2002 .

[20]  M. Ovidio,et al.  Diel activity of Sarpa salpa (Sparidae) by ultrasonic telemetry in a Posidonia oceanica meadow of Corsica (Mediterranean Sea) , 2002 .

[21]  S. M. Evans,et al.  The value of marine ecological data collected by volunteers , 2003 .

[22]  Brice X Semmens,et al.  Conservation and management applications of the REEF volunteer fish monitoring program. , 2003, Environmental monitoring and assessment.

[23]  K. Winemiller,et al.  Diel changeover in sandbank fish assemblages in a neotropical floodplain river , 2003 .

[24]  E. Morgan Ecological Significance of Biological Clocks , 2004 .

[25]  M. D. Marques,et al.  Rhythms and Ecology — Do Chronobiologists Still Remember Nature? , 2004 .

[26]  Douglas E. Colton,et al.  Diurnal variability in a fish assemblage of a Bahamian coral reef , 1981, Environmental Biology of Fishes.

[27]  M. Harmelin-Vivien,et al.  Temporal Fluctuations and Settlement Patterns of Native and Lessepsian Herbivorous Fishes on the Lebanese Coast (Eastern Mediterranean) , 2004, Environmental Biology of Fishes.

[28]  G. J. Piet,et al.  Diel variation in feeding and vertical distribution of ten co-occurring fish species: consequences for resource partitioning , 1997, Environmental Biology of Fishes.

[29]  S. G. Reebs Plasticity of diel and circadian activity rhythms in fishes , 2002, Reviews in Fish Biology and Fisheries.

[30]  E. Naylor,et al.  Chronobiology: implications for marine resource exploitation and management , 2005 .

[31]  N. Mrosovsky,et al.  Diurnal mice (Mus musculus) and other examples of temporal niche switching , 2005, Journal of Comparative Physiology A.

[32]  William J. Walsh,et al.  Impact of observers experience level on counts of fishes in underwater visual surveys , 2006 .

[33]  J. Aguzzi,et al.  Preliminary evidences of circadian fan activity rhythm in Sabella spallanzanii (Gmelin, 1791) (Polychaeta: Sabellidae) , 2006 .

[34]  G. Ardizzone,et al.  Diel feeding periodicity and daily ration of shelf break fish species , 2006, Journal of the Marine Biological Association of the United Kingdom.

[35]  Y. Cornet,et al.  Activity patterns, home-range size, and habitat utilization of Sarpa salpa (Teleostei: Sparidae) in the Mediterranean Sea , 2006 .

[36]  M. Milazzo,et al.  Diel variability in counts of reef fishes and its implications for monitoring , 2006 .

[37]  Ernesto Azzurro,et al.  Evaluating day–night changes in shallow Mediterranean rocky reef fish assemblages by visual census , 2007 .

[38]  W. Gladstone Requirements for marine protected areas to conserve the biodiversity of rocky reef fishes , 2007 .

[39]  M. Kasai,et al.  Circadian locomotor activity in Japanese sea catfish Plotosus lineatus , 2009, Fisheries Science.

[40]  R C Hermida,et al.  Chronobiological analysis techniques. Application to blood pressure , 2009, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[41]  R. Bonney,et al.  Citizen Science: A Developing Tool for Expanding Science Knowledge and Scientific Literacy , 2009 .

[42]  Jacopo Aguzzi,et al.  Modeled day-night biases in decapod assessment by bottom trawling survey , 2009 .

[43]  K. Last,et al.  Tidal, Daily, and Lunar‐Day Activity Cycles in the Marine Polychaete Nereis virens , 2009, Chronobiology international.

[44]  Aaron M Ellison,et al.  Observer bias and the detection of low-density populations. , 2009, Ecological applications : a publication of the Ecological Society of America.

[45]  J. Silvertown A new dawn for citizen science. , 2009, Trends in ecology & evolution.

[46]  J. Aguzzi,et al.  The influence of light availability and predatory behavior of the decapod crustacean Nephrops norvegicus on the activity rhythms of continental margin prey decapods , 2009 .

[47]  Christopher D. Stallings,et al.  Fishery-Independent Data Reveal Negative Effect of Human Population Density on Caribbean Predatory Fish Communities , 2009, PloS one.

[48]  J. Aguzzi,et al.  Sensory constraints in temporal segregation in two species of anglerfish, Lophius budegassa and L. piscatorius , 2010 .

[49]  Jacopo Aguzzi,et al.  Light Intensity Determines Temporal Niche Switching of Behavioral Activity in Deep-Water Nephrops norvegicus (Crustacea: Decapoda) , 2010, Journal of biological rhythms.

[50]  Paolo Menesatti,et al.  Behavioral rhythms of hydrocarbon seep fauna in relation to internal tides , 2010 .

[51]  David March,et al.  Short-term residence, home range size and diel patterns of the painted comber Serranus scriba in a temperate marine reserve , 2010 .

[52]  David N. Bonter,et al.  Citizen Science as an Ecological Research Tool: Challenges and Benefits , 2010 .

[53]  E. Arias-Castro,et al.  Large-Scale Absence of Sharks on Reefs in the Greater-Caribbean: A Footprint of Human Pressures , 2010, PloS one.

[54]  H. Lotze,et al.  Spatial and temporal trends in yellow stingray abundance: evidence from diver surveys , 2011, Environmental Biology of Fishes.

[55]  J. Caselle,et al.  Comparing volunteer and professionally collected monitoring data from the rocky subtidal reefs of Southern California, USA , 2012, Environmental Monitoring and Assessment.

[56]  R. Douglas,et al.  A latitudinal cline in the efficacy of endogenous signals: evidence derived from retinal cone contraction in fish , 2011, Journal of Experimental Biology.

[57]  Francesc Maynou,et al.  Tracking Signals of Change in Mediterranean Fish Diversity Based on Local Ecological Knowledge , 2011, PloS one.