Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries

This study investigated pH, activity and concentration of carbonic anhydrase VI (CA VI) in dental biofilm of caries and caries-free children of 7–9 years old. Seventy-four children were selected and divided into two groups. The caries diagnosis was performed according to the WHO criteria, including the early caries lesion. After biofilm collection and pH determination, CA VI concentration and activity were determined by ELISA and Zimography respectively. The data were submitted to a Mann-Whitney test and to Pearson and Spearman correlation analyses. Means and standard deviations of dental caries for the caries group were of 3.162 ± 1.385. The biofilm pH was significantly higher in the caries-free group. The CA VI activity was significantly higher in biofilm of children with caries. The CA VI concentration was significantly higher in biofilm of caries-free children. In caries-free children, there was a moderate negative correlation between CA VI activity and concentration in dental biofilm as well as between pH and CA VI activity. A negative correlation between biofilm pH and CA VI concentration was found in the caries group. In conclusion, CA VI was shown to be more active in the biofilm of school children with caries in order to contribute to neutralization of biofilm acid.

[1]  M. Nobre dos Santos,et al.  Children with a Higher Activity of Carbonic Anhydrase VI in Saliva Are More Likely to Develop Dental Caries , 2017, Caries Research.

[2]  L. P. Rodrigues,et al.  Relationship among &agr; amylase and carbonic anhydrase VI in saliva, visible biofilm, and early childhood caries: a longitudinal study , 2017, International journal of paediatric dentistry.

[3]  L. Samaranayake,et al.  Normal Oral Flora and the Oral Ecosystem. , 2017, Dental clinics of North America.

[4]  A. Sheiham,et al.  Diet and Dental Caries , 2015, Journal of dental research.

[5]  C. Cingi,et al.  Obstructive sleep apnoea syndrome does not negatively affect oral and dental health , 2015, The Journal of Laryngology & Otology.

[6]  V. Chandrasekhar,et al.  Effect of three different remineralizing agents on enamel caries formation--an in vitro study. , 2015, Kathmandu University medical journal.

[7]  W. James,et al.  A reappraisal of the quantitative relationship between sugar intake and dental caries: the need for new criteria for developing goals for sugar intake , 2014, BMC Public Health.

[8]  A. Dahlén,et al.  Dental plaque pH and ureolytic activity in children and adults of a low caries population , 2014, Acta odontologica Scandinavica.

[9]  A. Spencer,et al.  Water fluoridation and the association of sugar-sweetened beverage consumption and dental caries in Australian children. , 2013, American journal of public health.

[10]  M. Nobre dos Santos,et al.  Relationship among Salivary Carbonic Anhydrase VI Activity and Flow Rate, Biofilm pH and Caries in Primary Dentition , 2012, Caries Research.

[11]  U. Dixit,et al.  Role of plaque in the clearance of salivary sucrose and its influence on salivary ph. , 2011, Journal of the Indian Society of Pedodontics and Preventive Dentistry.

[12]  Philip D Marsh,et al.  Microbiology of dental plaque biofilms and their role in oral health and caries. , 2010, Dental clinics of North America.

[13]  L. Rodrigues,et al.  Relationship among microbiological composition and presence of dental plaque, sugar exposure, social factors and different stages of early childhood caries. , 2010, Archives of Oral Biology.

[14]  L. S. Mofatto,et al.  Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7–9 years , 2010, The Pharmacogenomics Journal.

[15]  K. Südekum,et al.  Carbonic anhydrase II is secreted from bovine parotid glands. , 2010, Histology and histopathology.

[16]  S. Akyüz,et al.  Association between dental-oral health in young adults and salivary glutathione, lipid peroxidation and sialic acid levels and carbonic anhydrase activity. , 2008, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[17]  Tony J Collins,et al.  ImageJ for microscopy. , 2007, BioTechniques.

[18]  P. Bebas,et al.  Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis (Lepidoptera: Noctuidae) controlled by circadian clock. , 2006, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[19]  A. Assaf,et al.  Effect of different diagnostic thresholds on dental caries calibration - a 12 month evaluation. , 2006, Community dentistry and oral epidemiology.

[20]  M. Asari,et al.  Immunohistochemistry of the bovine secretory carbonic anhydrase isozyme (CA-VI) in bovine alimentary canal and major salivary glands. , 2006, The Journal of veterinary medical science.

[21]  Y. Yura,et al.  A role of salivary carbonic anhydrase VI in dental plaque. , 2006, Archives of oral biology.

[22]  M. Nobre dos Santos,et al.  Relationship among Dental Plaque Composition, Daily Sugar Exposure and Caries in the Primary Dentition , 2002, Caries Research.

[23]  S. Parkkila,et al.  Salivary carbonic anhydrase isoenzyme VI , 1999, The Journal of physiology.

[24]  S. Parkkila,et al.  Salivary Carbonic Anhydrase Isoenzyme VI Is Located in the Human Enamel Pellicle , 1999, Caries Research.

[25]  Seppo Parkkila,et al.  A Low Concentration of Carbonic Anhydrase Isoenzyme VI in Whole Saliva Is Associated with Caries Prevalence , 1999, Caries Research.

[26]  W. Sly,et al.  Secretory carbonic anhydrase isoenzyme (CA VI) in human serum. , 1997, Clinical chemistry.

[27]  A. Bidani,et al.  Velocity of CO2 exchanges in the lungs. , 1988, Annual review of physiology.

[28]  I. Szabó.  Carbonic anhydrase activity in the saliva of children and its relation to caries activity. , 1974, Caries research.