An absolute method for protein determination based on difference in absorbance at 235 and 280 nm.
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[1] D. Wetlaufer,et al. Ultraviolet spectra Of Proteins and Amino Acids , 1963 .
[2] G. Schwert. The molecular size and shape of the pancreatic proteases. II. Chymotrypsinogen. , 1951, The Journal of biological chemistry.
[3] L. Fothergill,et al. Thiol and disulphide contents of hen ovalbumin. C-terminal sequence and location of disulphide bond. , 1970, The Biochemical journal.
[4] William W. Cohen,et al. Evidence for an Active-Center Histidine in Trypsin through Use of a Specific Reagent, 1-Chloro-3-tosylamido-7-amino-2-heptanone, the Chloromethyl Ketone Derived from Nα-Tosyl-L-lysine* , 1965 .
[5] E. Fischer,et al. The amino acid composition of alpha-amylase from Bacillus subtilis. , 1959, The Journal of biological chemistry.
[6] J. Sedmak,et al. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. , 1977, Analytical biochemistry.
[7] Kalckar Hm,et al. Differential spectrophotometry of purine compounds by means of specific enzymes; studies of the enzymes of purine metabolism. , 1947 .
[8] J. Potts,et al. Conformational studies of pancreatic ribonuclease and its subtilisin-produced derivatives. , 1965, The Journal of biological chemistry.
[9] O. H. Lowry,et al. Measurement of nanogram quantities of protein by hydrolysis followed by reaction with orthophthalaldehyde or determination of glutamate. , 1976, Analytical biochemistry.
[10] M. M. Bradford. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.
[11] A. Gornall,et al. Determination of serum proteins by means of the biuret reaction. , 1949, The Journal of biological chemistry.
[12] J. Miller,et al. Photometric analysis of proteins and peptides at 191-194 millimicrons. , 1970, Analytical biochemistry.
[13] L. Mokrasch. Determination of small amounts of protein in total lipid extracts by the use of trinitrobenzenesulfonic acid. , 1970, Analytical biochemistry.
[14] A. Goldfarb,et al. The ultraviolet absorption spectra of proteins. , 1951, The Journal of biological chemistry.
[15] O. H. Lowry,et al. Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.
[16] R. Bernlohr,et al. A new spectrophotometric assay for protein in cell extracts. , 1977, Analytical biochemistry.
[17] E. L. Mccandless,et al. Simple, rapid, turbidometric determination of inorganic sulfate and/or protein. , 1978, Analytical biochemistry.
[18] A. Habeeb,et al. Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. , 1966, Analytical biochemistry.
[19] M. Crumpton,et al. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION. , 1963, The Biochemical journal.
[20] T. Peters,et al. Isolation of two large peptide fragments from the amino- and carboxyl-terminal positions of bovine serum albumin. , 1967, The Journal of biological chemistry.
[21] C. S. Yeh. Rapid microdetermination of nitrogen by the Dumas method , 1966 .
[22] R K Scopes,et al. Measurement of protein by spectrophotometry at 205 nm. , 1974, Analytical biochemistry.
[23] M. J. Johnson. A rapid micromethod for estimation of non-volatile organic matter. , 1949, The Journal of biological chemistry.
[24] S. Colowick,et al. Methods in Enzymology , Vol , 1966 .
[25] H. Edelhoch,et al. Spectroscopic determination of tryptophan and tyrosine in proteins. , 1967, Biochemistry.
[26] F. C. Davis,et al. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. , 1968, Analytical biochemistry.
[27] F. Šorm,et al. Nitration of tyrosine residues in the pancreatic trypsin inhibitor with tetranitromethane. , 1968, European journal of biochemistry.
[28] Cunningham Lw,et al. Physical and Chemical Studies of a Limited Reaction of Iodine with Proteins , 1959 .
[29] W. J. Waddell,et al. A simple ultraviolet spectrophotometric method for the determination of protein. , 1956, The Journal of laboratory and clinical medicine.
[30] P. E. Wilcox,et al. Amino acid composition of alpha-chymotrypsinogen, including estimation of asparagine and glutamine. , 1957, The Journal of biological chemistry.
[31] Marvin J. Johnson. ISOLATION AND PROPERTIES OF A PURE YEAST POLYPEPTIDASE , 1941 .