The NF 1 tumor suppressor critically regulates TSC 2 and mTOR

CELL BIOLOGY. For the article ‘‘The NF1 tumor suppressor critically regulates TSC2 and mTOR,’’ by Cory M. Johannessen, Elizabeth E. Reczek, Marianne F. James, Hilde Brems, Eric Legius, and Karen Cichowski, which appeared in issue 24, June 14, 2005, of Proc. Natl. Acad. Sci. USA (102, 8573–8578; first published June 3, 2005; 10.1073 pnas.0503224102), the authors note the following. On page 8574, the last sentence of the first paragraph in the left column, ‘‘Clarified lysates were normalized for protein levels and analyzed by Western blotting with the following antibodies: phospho-p70S6K (T-389), phosphotuberin (S-939), phospho-tuberin (T-1462), phospho-Akt (S473), tuberin (C-20) from Santa Cruz Biotechnology, and protein kinase B AKT1, actin, and -tubulin from SigmaAldrich,’’ should read: ‘‘Clarified lysates were normalized for protein levels and analyzed by Western blotting with the following antibodies: phospho-p70S6K (T-389), phospho-tuberin (S939), phospho-tuberin (T-1462), phospho-Akt (S-473) from Cell Signaling Technologies, tuberin (C-20) from Santa Cruz Biotechnology, and protein kinase B AKT1, actin, and -tubulin from Sigma-Aldrich.’’ On page 8574, the second sentence of the second paragraph in the left column, ‘‘For lentiviral production, HEK293T cells were transfected with the 8.2 lentiviral construct (encoding gag, pol, rev), VSVG, and either empty pKO vector or the pKO vector containing the following sequence transcribing short-hairpin RNAs specific to NF1: 5 -TTATAAATAGCCTGGAAAAGG-3 ,’’ should read: ‘‘For lentiviral production, HEK293T cells were transfected with the 8.2 lentiviral construct (encoding gag, pol, rev), VSVG, and either empty pLKO.1puro vector or the pLKO.1puro vector containing the following sequence transcribing short-hairpin RNAs specific to NF1: 5 -TTATAAATAGCCTGGAAAAGG-3 .’’ In addition, the authors would like to add the following statement to the Acknowledgments: ‘‘We would also like to thank Dr. William C. Hahn (Harvard Medical School and Dana–Farber Cancer Institute, Boston) and the Broad Institute (Cambridge, MA) for the pLKO.1 lentiviral constructs.’’