THE PROTECTIVE EFFECT OF INDOMETHACINE ON SULFUR MUSTARD-INDUCED OCULAR DAMAGE IN THE RABBIT EYE

Sulfur mustard, a bifunctional alkylating agent, causes severe eye injury. The injury is a result of late inflammation, which is mediated by prostaglandins. Theo­ retically, inhibition of prostaglandins by reagents such as indomethacine could result in alleviation of clinical adverse effects. In this study, the protective effect of topically applied indomethacine against sulfur mustard toxicity was assessed on the rabbit eye. Ocular toxicity was studied by using light microscopy, electron microscopy and visual inspection methods. Methods used in this assay showed that 100 ilL of indomethacine solution (0.5% W N) two hours before exposure and throughout the experiment could markedly decrease the toxic effects of mus­ tard. MJIRI, Vol. 14, No.4, 385-391, 2001.

[1]  M. Akhgari,et al.  The involvement of chemical mediators; Substance-p and prostaglandins in ophthalmic effects of mustards , 1998 .

[2]  H. Inoue,et al.  Mechanism of mustard oil-induced skin inflammation in mice. , 1997, European journal of pharmacology.

[3]  S. Galli,et al.  Substance P-induced augmentation of cutaneous vascular permeability and granulocyte infiltration in mice is mast cell dependent. , 1989, The Journal of clinical investigation.

[4]  H. Uusitalo,et al.  Calcitonin gene-related peptide (CGRP) immunoreactive sensory nerves in the human and guinea pig uvea and cornea. , 1989, Experimental eye research.

[5]  X. Hua,et al.  Capsaicin induced release of multiple tachykinins (substance P, neurokinin A and eledoisin-like material) from guinea-pig spinal cord and ureter , 1986, Neuroscience.

[6]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[7]  R. Beuerman,et al.  Density and organization of free nerve endings in the corneal epithelium of the rabbit , 1982, Pain.

[8]  C. Belmonte,et al.  Responses of cat corneal sensory receptors to mechanical and thermal stimulation. , 1981, The Journal of physiology.

[9]  P. Bhattacherjee.  Prostaglandins and inflammatory reactions in the eye. , 1980, Methods and findings in experimental and clinical pharmacology.

[10]  L. Jampol,et al.  Pathways for the response of the eye to injury. , 1975, Investigative ophthalmology.

[11]  L. Bito,et al.  The effects of experimental uveitis on anterior uveal prostaglandin transport and aqueous humor composition. , 1974, Investigative ophthalmology.

[12]  L. Jampol,et al.  Aspirin Prevents the Disruption of the Blood–Aqueous Barrier in the Rabbit Eye , 1972, Nature.

[13]  W. Unger.  Mediation of the ocular response to injury and irritation: peptides versus prostaglandins. , 1989, Progress in clinical and biological research.

[14]  K. Eakins,et al.  Prostaglandin and non-prostaglandin mediated breeakdown of the blood-aqueous barrier. , 1977, Experimental eye research.

[15]  N. Jancsó.  DESENSITIZATION WITH CAPSAICIN AND RELATED ACYLAMIDES AS A TOOL FOR STUDYING THE FUNCTION OF PAIN RECEPTORS , 1968 .