The biological effects of antiadhesion agents on activated RAW264.7 macrophages.

The objective of this study is to determine the biological effects of various antiadhesion agents on macrophages, which play an essential role in wound healing and adhesion. To determine these effects, RAW264.7 macrophages were activated with lipopolysaccharide in the presence of antiadhesion agents: oxidized regenerated cellulose (oxyC), sodium hyaluronate (HA), dexamethasone (Dex), or chondroitin sulfate (CS). The release of nitric oxide (NO), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), or matrix metalloproteinases (MMPs) from RAW264.7 was measured. We found that oxyC reduced the release of NO, IL-6, MMP-2, and MMP-9, whereas it enhanced the release of VEGF. HA reduced the release of MMP-2, whereas it enhanced the release of VEGF and NO. HA exhibited no significant effect on the release of IL-6 or MMP-9. Dex reduced the release of NO, VEGF, IL-6, MMP-2, and MMP-9. CS reduced the release of VEGF, IL-6, and MMP-2, although it had no significant effect on the release of NO and MMP-9. Antiadhesion agents, which have been clinically used as physical barriers, modulated the functions of macrophages.

[1]  G. Saed,et al.  Molecular characterization of fibroblasts isolated from human peritoneum and adhesions. , 2001, Fertility and sterility.

[2]  T. Kudo,et al.  Nafamostat mesilate, a serine protease inhibitor, suppresses lipopolysaccharide-induced nitric oxide synthesis and apoptosis in cultured human trophoblasts. , 2000, Life sciences.

[3]  U. Rout,et al.  Altered Expressions of VEGF mRNA Splice Variants During Progression of Uterine‐Peritoneal Adhesions in the Rat , 2000, American journal of reproductive immunology.

[4]  F. Cerra,et al.  Modulation of macrophage and B cell function by glycosaminoglycans , 1999, Journal of leukocyte biology.

[5]  N. Chegini,et al.  Peritoneal fluid cytokine and eicosanoid levels and their relation to the incidence of peritoneal adhesion. , 1999 .

[6]  T. Kudo,et al.  Generation of peroxynitrite and apoptosis in placenta of patients with chorioamnionitis: possible implications in placental abruption. , 1999, Human reproduction.

[7]  İ. Koçak,et al.  The comparison of gonadotropin-releasing hormone agonist therapy and intraperitoneal Ringer's lactate solution in prevention of postoperative adhesion formation in rat models. , 1999, European journal of obstetrics, gynecology, and reproductive biology.

[8]  L. Moldawer,et al.  Metalloproteinase inhibitors and wound healing: a novel enhancer of wound strength. , 1998, Surgery.

[9]  P. Singhal,et al.  Effect of vascular endothelial growth factor on nitric oxide production by cultured rat mesangial cells. , 1998, Biochemical and biophysical research communications.

[10]  J. Jesty,et al.  Vascular endothelial groth factor induces tissue factor and matrix metalloproteinase production in endothelial cells: Conversion of prothrombin to thrombin results in progelatininase a activation and cell proliferation , 1998 .

[11]  M. Diamond,et al.  Reduction of postsurgical adhesion formation in the rabbit uterine horn model with use of hyaluronate/carboxymethylcellulose gel. , 1998, Fertility and sterility.

[12]  C. Miyaura,et al.  Regulation of matrix metalloproteinases (MMP-2, -3, -9, and -13) by interleukin-1 and interleukin-6 in mouse calvaria: association of MMP induction with bone resorption. , 1998, Endocrinology.

[13]  S. Perry,et al.  Abdominal adhesiolysis: inpatient care and expenditures in the United States in 1994. , 1998, Journal of the American College of Surgeons.

[14]  S. Parthasarathy,et al.  Interaction of Interceed oxidized regenerated cellulose with macrophages: a potential mechanism by which Interceed may prevent adhesions. , 1997, American journal of obstetrics and gynecology.

[15]  J. Isner,et al.  Vascular endothelial growth factor/vascular permeability factor produces nitric oxide-dependent hypotension. Evidence for a maintenance role in quiescent adult endothelium. , 1997, Arteriosclerosis, thrombosis, and vascular biology.

[16]  D. Falcone,et al.  Role of Laminin in Matrix Induction of Macrophage Urokinase-type Plasminogen Activator and 92-kDa Metalloproteinase Expression* , 1997, The Journal of Biological Chemistry.

[17]  M. Burdick,et al.  Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44. , 1996, The Journal of clinical investigation.

[18]  E. Ruoslahti,et al.  Serglycin‐binding proteins in activated macrophages and platelets , 1996, Journal of leukocyte biology.

[19]  G. Neufeld,et al.  Interleukin 6 Induces the Expression of Vascular Endothelial Growth Factor (*) , 1996, The Journal of Biological Chemistry.

[20]  R. F. Morgan,et al.  Analysis of the kinetics of peritoneal adhesion formation in the rat and evaluation of potential antiadhesive agents. , 1995, Surgery.

[21]  G. Murrell,et al.  Nitric oxide activates metalloprotease enzymes in articular cartilage. , 1995, Biochemical and biophysical research communications.

[22]  H. Martikainen,et al.  The efficacy of Interceed(TC7)* for prevention of reformation of postoperative adhesions on ovaries, fallopian tubes, and fimbriae in microsurgical operations for fertility: a multicenter study. Nordic Adhesion Prevention Study Group. , 1995, Fertility and sterility.

[23]  G. Vlahakes,et al.  Prevention of postoperative pericardial adhesions with a hyaluronic acid coating solution. Experimental safety and efficacy studies. , 1994, The Journal of thoracic and cardiovascular surgery.

[24]  G. diZerega.  Contemporary adhesion prevention. , 1994, Fertility and sterility.

[25]  A. Baldwin,et al.  The I kappa B proteins: multifunctional regulators of Rel/NF-kappa B transcription factors. , 1993, Genes & development.

[26]  A. Haney,et al.  Comparison of the peritoneal cells elicited by oxidized regenerated cellulose (Interceed) and expanded polytetrafluoroethylene (Gore-Tex Surgical Membrane) in a murine model. , 1992, American journal of obstetrics and gynecology.

[27]  M. Diamond,et al.  Adhesion reformation and de novo adhesion formation after reproductive pelvic surgery. , 1987, Fertility and sterility.