A multimodal nanoparticle for preoperative magnetic resonance imaging and intraoperative optical brain tumor delineation.

The determination of brain tumor margins both during the presurgical planning phase and during surgical resection has long been a challenging task in the therapy of brain tumor patients. Using a model of gliosarcoma with stably green fluorescence protein-expressing 9L glioma cells, we explored a multimodal (near-infrared fluorescent and magnetic) nanoparticle as a preoperative magnetic resonance imaging contrast agent and intraoperative optical probe. Key features of nanoparticle metabolism, namely intracellular sequestration by microglia and the combined optical and magnetic properties of the probe, allowed delineation of brain tumors both by preoperative magnetic resonance imaging and by intraoperative optical imaging. This prototypical multimodal nanoparticle has unique properties that may allow radiologists and neurosurgeons to see the same probe in the same cells and may offer a new approach for obtaining tumor margins.

[1]  Michael Schulder,et al.  Intraoperative magnetic resonance imaging: impact on brain tumor surgery. , 2003, Cancer control : journal of the Moffitt Cancer Center.

[2]  Vasilis Ntziachristos,et al.  Shedding light onto live molecular targets , 2003, Nature Medicine.

[3]  Ralph Weissleder,et al.  Feasibility of in vivo multichannel optical imaging of gene expression: experimental study in mice. , 2002, Radiology.

[4]  Ralph Weissleder,et al.  Magnetic Nanosensors for the Detection of Oligonucleotide Sequences. , 2001, Angewandte Chemie.

[5]  Anita Mahadevan-Jansen,et al.  In Vivo Brain Tumor Demarcation Using Optical Spectroscopy¶ , 2001, Photochemistry and photobiology.

[6]  M. Knauth,et al.  Low-field interventional MRI in neurosurgery: finding the right dose of contrast medium , 2001, Neuroradiology.

[7]  C Zimmer,et al.  Magnetic labeling of activated microglia in experimental gliomas. , 2001, Neoplasia.

[8]  C R Wirtz,et al.  Monocrystalline iron oxide nanoparticles: possible solution to the problem of surgically induced intracranial contrast enhancement in intraoperative MR imaging. , 2001, AJNR. American journal of neuroradiology.

[9]  C Zimmer,et al.  Pharmacokinetic analysis of glioma compartments with dynamic Gd-DTPA-enhanced magnetic resonance imaging. , 2000, Magnetic resonance imaging.

[10]  V. Ntziachristos,et al.  Concurrent MRI and diffuse optical tomography of breast after indocyanine green enhancement. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[11]  C R Wirtz,et al.  Surgically induced intracranial contrast enhancement: potential source of diagnostic error in intraoperative MR imaging. , 1999, AJNR. American journal of neuroradiology.

[12]  R Weissleder,et al.  High-efficiency intracellular magnetic labeling with novel superparamagnetic-Tat peptide conjugates. , 1999, Bioconjugate chemistry.

[13]  R Weissleder,et al.  Improved delineation of human brain tumors on MR images using a long‐circulating, superparamagnetic iron oxide agent , 1999, Journal of magnetic resonance imaging : JMRI.

[14]  R Weissleder,et al.  Novel gliosarcoma cell line expressing green fluorescent protein: A model for quantitative assessment of angiogenesis. , 1998, Microvascular research.

[15]  H Stepp,et al.  Intraoperative detection of malignant gliomas by 5-aminolevulinic acid-induced porphyrin fluorescence. , 1998, Neurosurgery.

[16]  L. Josephson,et al.  Use of AMI-227 as an oral MR contrast agent. , 1994, Magnetic resonance imaging.

[17]  R Weissleder,et al.  Monocrystalline iron oxide nanocompounds (MION): Physicochemical properties , 1993, Magnetic resonance in medicine.

[18]  R Weissleder,et al.  Superparamagnetic iron oxide: pharmacokinetics and toxicity. , 1989, AJR. American journal of roentgenology.

[19]  V Blazek,et al.  Optical properties of human brain tissue, meninges, and brain tumors in the spectral range of 200 to 900 nm. , 1987, Neurosurgery.

[20]  J. Thiessen,et al.  Indocyanine green pharmacokinetics in the rabbit. , 1984, Canadian journal of physiology and pharmacology.