Exogenous supplementation of papain as growth promoter in diet of fingerlings of Cyprinus carpio

The effect of different levels of papain (1%, 2% and 4%) supplemented feed in Cyprinus carpio on growth rate, nutrient digestibility, gross protein retention (GPR), feed conversion ratio (FCR), protein efficiency ratio (PER), apparent net protein utilization (ANPU), energy conversion efficiency (ECE%) and nitrogen retention efficiency (NRE) was studied. The fish fed on the feed supplemented with 2% papain treatment resulted in lowest feed conversion ratio (FCR), better growth rate, high protein digestibility, higher protein efficiency ratio (PER), good gross energy retention, better apparent net protein utilization (ANPU), energy conversion efficiency ECE (%) and nitrogen retention efficiency (NRE). All these parameters were lowest in case of control (P > 0.05). Maximum growth rate, nutrient digestibility, gross protein retention (GPR), feed conversion ratio (FCR), protein efficiency ratio (PER), apparent net protein utilization (ANPU), energy conversion efficiency (ECE %) and nitrogen retention efficiency (NRE) were seen during the 8 week of the study period and 2% papain level showed best results (P < 0.05). The physicochemical parameters were within the optimum range as desired for fish culture practices. Papain did not affect the water quality parameters and had no adverse effect on feeding response of fish. Thus, supplementation of papain to feed of common carp at a level of 2% resulted in better growth performance.

[1]  E. McLean,et al.  The effects of organic protein supplementation upon growth, feed conversion and texture quality parameters of juvenile cobia (Rachycentron canadum) , 2007 .

[2]  B. C. Patra,et al.  Effect of varying dietary protein levels on growth and production of Chanos chanos (Forsskal) in inland saline groundwater: laboratory and field studies , 2006, Aquaculture International.

[3]  F. Liebert,et al.  Nutrient utilization of Nile tilapia Oreochromis niloticus fed plant based low phosphorus diets supplemented with graded levels of different sources of microbial phytase , 2005 .

[4]  K. Becker,et al.  Preliminary nutritional evaluation of Mucuna seed meal (Mucuna pruriens var. utilis) in common carp (Cyprinus carpio L.): an assessment by growth performance and feed utilisation , 2001 .

[5]  E. D’Agaro,et al.  Use of nonlinear regression to evaluate the effects of phytase enzyme treatment of plant protein diets for rainbow trout (Oncorhynchus mykiss) , 1998 .

[6]  M. Izquierdo,et al.  Effects of live food and dietary digestive enzymes on the efficiency of microdiets for seabass (Dicentrarchus labrax) larvae , 1997 .

[7]  M. Wong,et al.  The use of enzyme-digested soybean residue for feeding common carp. , 1996, Biomedical and environmental sciences : BES.

[8]  H. Classen Cereal grain starch and exogenous enzymes in poultry diets , 1996 .

[9]  E. Robinson,et al.  Use of Microbial Phytase in Channel Catfish Ictalurus punctatus Diets to Improve Utilization of Phytate Phosphorus , 1996 .

[10]  Sena S. De Silva,et al.  Interactions of varying dietary protein and lipid levels in young red tilapia: Evidence of protein sparing , 1991 .

[11]  Mostafa A. R. Hossain,et al.  Nutritional evaluation of some Bangladeshi oilseed meals as partial substitutes for fish meal in the diet of common carp, Cyprinus carpio L. , 1989 .

[12]  N L Richardson,et al.  Influence of dietary calcium, phosphorus, zinc and sodium phytate level on cataract incidence, growth and histopathology in juvenile chinook salmon (Oncorhynchus tshawytscha). , 1985, The Journal of nutrition.

[13]  K. Dąbrowski,et al.  A study of the application of proteolytic enzymes to fish food , 1977 .

[14]  I. Liener,et al.  Contribution of trypsin inhibitors to the deleterious effects of unheated soybeans fed to rats. , 1973, The Journal of nutrition.

[15]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[16]  Keshun Liu,et al.  Chemistry and Nutritional Value of Soybean Components , 1997 .

[17]  V. Sugunan Reservoir Fisheries of India , 1995 .

[18]  A. Tacon Standards methods for the nutrition and feeding of farmed fish and shrimp , 1990 .

[19]  J. C. Wekell,et al.  The effect of phytates on the growth of rainbow trout (Salmo gairdneri) fed purified diets containing varying quantities of calcium and magnesium , 1983 .

[20]  J. M. Watt,et al.  The medicinal and poisonous plants of southern and eastern Africa. , 1962 .

[21]  E. L. Smith,et al.  The properties of papain. , 1957, Advances in enzymology and related subjects of biochemistry.