Surface Analyses and Immune Reactivities of Major Cell Wall-Associated Proteins of Group A Streptococcus

ABSTRACT A proteomic analysis was undertaken to identify cell wall-associated proteins of Streptococcus pyogenes. Seventy-four distinct cell wall-associated proteins were identified, 66 of which were novel. Thirty-three proteins were immunoreactive with pooled S. pyogenes-reactive human antisera. Biotinylation of the GAS cell surface identified 23 cell wall-associated proteins that are surface exposed.

[1]  Yinduo Ji,et al.  Impact of M49, Mrp, Enn, and C5a Peptidase Proteins on Colonization of the Mouse Oral Mucosa by Streptococcus pyogenes , 1998, Infection and Immunity.

[2]  R. Lottenberg,et al.  The plasmin-binding protein Plr of group A streptococci is identified as glyceraldehyde-3-phosphate dehydrogenase. , 1996, Microbiology.

[3]  R. Lancefield,et al.  ANTIGENIC DIFFERENCES BETWEEN MATT HEMOLYTIC STREPTOCOCCI AND THEIR GLOSSY VARIANTS , 1928, The Journal of experimental medicine.

[4]  E. Ziomek,et al.  Influence of group A streptococcal acid glycoprotein on expression of major virulence factors and internalization by epithelial cells. , 2003, Microbial pathogenesis.

[5]  J. Musser,et al.  Characterization of Two Novel Pyrogenic Toxin Superantigens Made by an Acute Rheumatic Fever Clone of Streptococcus pyogenes Associated with Multiple Disease Outbreaks , 2002, Infection and Immunity.

[6]  M. Sund,et al.  Arginine catabolism by strains of oral streptococci , 1990, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[7]  M. Cunningham,et al.  Pathogenesis of group A streptococcal infections. , 2000, Clinical microbiology reviews.

[8]  R. Novick.  Sortase: the surface protein anchoring transpeptidase and the LPXTG motif. , 2000, Trends in microbiology.

[9]  V. Fischetti,et al.  Role of the C-Terminal Lysine Residues of Streptococcal Surface Enolase in Glu- and Lys-Plasminogen-Binding Activities of Group A Streptococci , 2004, Infection and Immunity.

[10]  V. Fischetti,et al.  Glyceraldehyde-3-phosphate dehydrogenase on the surface of group A streptococci is also an ADP-ribosylating enzyme. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Malak Kotb,et al.  Inverse Relation between Disease Severity and Expression of the Streptococcal Cysteine Protease, SpeB, among Clonal M1T1 Isolates Recovered from Invasive Group A Streptococcal Infection Cases , 2000, Infection and Immunity.

[12]  A. Podbielski,et al.  The SpeB virulence factor of Streptococcus pyogenes, a multifunctional secreted and cell surface molecule with strepadhesin, laminin‐binding and cysteine protease activity , 2001, Molecular microbiology.

[13]  V. Fischetti,et al.  Streptococcal M protein: molecular design and biological behavior , 1989, Clinical Microbiology Reviews.

[14]  J. Carapetis,et al.  Skin infections and infestations in Aboriginal communities in northern Australia , 2000, The Australasian journal of dermatology.

[15]  G. Dougan,et al.  Characterization of an Isogenic Mutant ofStreptococcus pyogenes Manfredo Lacking the Ability To Make Streptococcal Acid Glycoprotein , 2000, Infection and Immunity.

[16]  J. Rosch,et al.  A Microdomain for Protein Secretion in Gram-Positive Bacteria , 2004, Science.

[17]  J. McArthur,et al.  Streptococcus pyogenes prtFII, but not sfbI, sfbII or fbp54, is represented more frequently among invasive-disease isolates of tropical Australia , 2002, Epidemiology and Infection.

[18]  V. Pancholi,et al.  Plasminogen-mediated group A streptococcal adherence to and pericellular invasion of human pharyngeal cells. , 2003, Microbial pathogenesis.

[19]  G. S. Chhatwal.  Anchorless adhesins and invasins of Gram-positive bacteria: a new class of virulence factors. , 2002, Trends in microbiology.

[20]  S. Cordwell,et al.  Characterisation of basic proteins from Spiroplasma melliferum using novel immobilised pH gradients , 1997, Electrophoresis.

[21]  M. Giambiagi-deMarval,et al.  Expression of heat-shock proteins in Streptococcus pyogenes and their immunoreactivity with sera from patients with streptococcal diseases. , 1998, Journal of medical microbiology.

[22]  A. Whatmore.  Streptococcus pyogenes sclB encodes a putative hypervariable surface protein with a collagen-like repetitive structure. , 2001, Microbiology.

[23]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[24]  V. Fischetti,et al.  Regulation of the Phosphorylation of Human Pharyngeal Cell Proteins by Group A Streptococcal Surface Dehydrogenase: Signal Transduction between Streptococci and Pharyngeal Cells , 1997, The Journal of experimental medicine.

[25]  Immune response to superoxide dismutase in group A streptococcal infection. , 2004, FEMS immunology and medical microbiology.

[26]  V. Fischetti,et al.  A major surface protein on group A streptococci is a glyceraldehyde-3- phosphate-dehydrogenase with multiple binding activity , 1992, The Journal of experimental medicine.

[27]  M. Rohde,et al.  Identification and Characterization of Two Temperature-Induced Surface-Associated Proteins of Streptococcus suis with High Homologies to Members of the Arginine Deiminase System of Streptococcus pyogenes , 2002, Journal of bacteriology.

[28]  V. Fischetti,et al.  Antibodies to streptococcal surface enolase react with human alpha-enolase: implications in poststreptococcal sequelae. , 2000, The Journal of infectious diseases.

[29]  A. Podbielski,et al.  A secreted streptococcal cysteine protease can cleave a surface-expressed M1 protein and alter the immunoglobulin binding properties. , 1998, Research in microbiology.

[30]  Jian Cai,et al.  Fluoride Exposure Attenuates Expression of Streptococcus pyogenes Virulence Factors* , 2002, The Journal of Biological Chemistry.

[31]  J. Musser,et al.  Identification of Rgg-Regulated Exoproteins ofStreptococcus pyogenes , 2001, Infection and Immunity.

[32]  Rita Kansal,et al.  Invasive M1T1 group A Streptococcus undergoes a phase‐shift in vivo to prevent proteolytic degradation of multiple virulence factors by SpeB , 2003, Molecular microbiology.

[33]  J. Altin,et al.  A one-step procedure for biotinylation and chemical cross-linking of lymphocyte surface and intracellular membrane-associated molecules. , 1995, Analytical biochemistry.

[34]  D. Beighton,et al.  Effect of Acidic pH on Expression of Surface-Associated Proteins of Streptococcus oralis , 2003, Applied and Environmental Microbiology.

[35]  Y. Hasegawa,et al.  Two-dimensional gel electrophoresis analysis of the abundance of virulent exoproteins of group A streptococcus caused by environmental changes , 2003, Archives of Microbiology.

[36]  J. Arnold,et al.  Molecular evolution of a multigene family in group A streptococci. , 1994, Molecular biology and evolution.

[37]  R. Feldman,et al.  Identification of Major Outer Surface Proteins of Streptococcus agalactiae , 2002, Infection and Immunity.

[38]  V. Fischetti,et al.  α-Enolase, a Novel Strong Plasmin(ogen) Binding Protein on the Surface of Pathogenic Streptococci* , 1998, The Journal of Biological Chemistry.

[39]  J. Musser,et al.  Identification and Immunogenicity of Group AStreptococcus Culture Supernatant Proteins , 2000, Infection and Immunity.