Characterisation of mycelial morphology using image analysis.

Image analysis is now well established in quantifying and characterising microorganisms from fermentation samples. In filamentous fermentations it has become an invaluable tool for characterising complex mycelial morphologies, although it is not yet used extensively in industry. Recent method developments include characterisation of spore germination from the inoculum stage and of the subsequent dispersed and pellet forms. Further methods include characterising vacuolation and simple structural differentiation of mycelia, also from submerged cultures. Image analysis can provide better understanding of the development of mycelial morphology, of the physiological states of the microorganisms in the fermenter, and of their interactions with the fermentation conditions. This understanding should lead to improved design and operation of mycelial fermentations.

[1]  S E Vecht-Lifshitz,et al.  Biotechnological applications of image analysis: present and future prospects. , 1992, Journal of biotechnology.

[2]  Pascal Formisyn,et al.  A simple staining procedure for the characterisation of Basidiomycetes pellets by image analysis , 1994 .

[3]  J. Prosser,et al.  A mathematical model for the growth of mycelial pellet populations , 1995, Biotechnology and bioengineering.

[4]  Jean Serra,et al.  Image Analysis and Mathematical Morphology , 1983 .

[5]  J. Smith,et al.  Filamentous growth of Aspergillus niger in submerged shake culture , 1969 .

[6]  Thomas,et al.  Applications of image analysis in cell technology. , 1996, Current opinion in biotechnology.

[7]  E D Gilles,et al.  Measurement and simulation of the morphological development of filamentous microorganisms , 1992, Biotechnology and bioengineering.

[8]  U. Reichl,et al.  Characterization of pellet morphology during submerged growth of Streptomyces tendae by image analysis , 1992, Biotechnology and bioengineering.

[9]  G. C. Paul,et al.  A structured model for hyphal differentiation and penicillin production using Penicillium chrysogenum , 2000, Biotechnology and bioengineering.

[10]  Y. Deo,et al.  Semicontinuous and continuous production of penicillin‐G by Penicillium chrysogenum cells immobilized in κ‐carrageenan beads , 1984, Biotechnology and bioengineering.

[11]  C L Cooney,et al.  Growth monitoring and control through computer‐aided on‐line mass balancing in a fed‐batch penicillin fermentation , 1983, Biotechnology and bioengineering.

[12]  C. Thomas,et al.  Fully‐Automatic Measurement of Mycelial Morphology by Image Analysis , 1992 .

[13]  Z. Fencl,et al.  Dependence of release of nucleotides from fungi on fermentor turbine speed , 1980 .

[14]  M. Pons,et al.  Characterization of Penicillium chrysogenum physiology in submerged cultures by color and monochrome image analysis , 1995, Biotechnology and bioengineering.

[15]  C. Thomas,et al.  Inoculum effects on fungal morphology: Shake flasks vs agitated bioreactors , 1994 .

[16]  A P Trinci,et al.  A model for hyphal growth and branching. , 1979, Journal of general microbiology.

[17]  J. Nielsen,et al.  Hyphal growth and fragmentation of Penicillium chrysogenum in submerged cultures , 1995, Biotechnology and bioengineering.

[18]  D I Wang,et al.  Computer control of the penicillin fermentation using the filtration probe in conjunction with a structured process model , 1983, Biotechnology and bioengineering.

[19]  E Keshavarz-Moore,et al.  Estimation of cell volume and biomass of penicillium chrysogenum using image analysis , 1992, Biotechnology and bioengineering.

[20]  J. Russ.  Computer-Assisted Microscopy , 1990, Springer US.

[21]  J. Prosser,et al.  Growth mechanisms and growth kinetics of filamentous microorganisms. , 1991, Critical reviews in biotechnology.

[22]  Peter Ramm,et al.  Advanced image analysis systems in cell, molecular and neurobiology applications , 1994, Journal of Neuroscience Methods.

[23]  E. W. de Bruijn,et al.  Methods for quantitative representation of the morphology of molds , 1981 .

[24]  Y. Hadar,et al.  A model for pellet size distributions in submerged mycelial cultures , 1983 .

[25]  E. Gilles,et al.  Mathematical model for apical growth, septation, and branching of mycelial microorganisms , 1992, Biotechnology and bioengineering.

[26]  P. Cox,et al.  Classification and measurement of fungal pellets by automated image analysis , 1992, Biotechnology and bioengineering.

[27]  C. A. Kent,et al.  Viability testing and characterization of germination of fungal spores by automatic image analysis , 1993, Biotechnology and bioengineering.

[28]  C. R. Thomas,et al.  The use of image analysis for morphological measurements on filamentous microorganisms. , 1988, Biotechnology and bioengineering.

[29]  C. A. Kent,et al.  Hyphal vocuolation and fragmentation inpenicillium chrysogenum. , 1994, Biotechnology and bioengineering.

[30]  U. Reichl,et al.  Study of the early growth and branching of Streptomyces tendae by means of an image processing system , 1990, Journal of microscopy.

[31]  C. R. Thomas,et al.  Morphological measurements on filamentous microorganisms by fully automatic image analysis , 1990, Biotechnology and bioengineering.

[32]  J. Nielsen,et al.  Pellet Formation and Fragmentation in Submerged Cultures of Penicillium chrysogenum and Its Relation to Penicillin Production , 1995, Biotechnology progress.

[33]  Philip W. Cox,et al.  Improved image analysis algorithm for the characterisation of mycelial aggregates after staining , 1994 .

[34]  M. Zalokar.  ENZYME ACTIVITY AND CELL DIFFERENTIATION IN NEUROSPORA , 1959 .

[35]  Marie-Noëlle Pons,et al.  Growth Monitoring of Filamentous Microorganisms by Image Analysis , 1992 .

[36]  M. Zalokar.  GROWTH AND DIFFERENTIATION OF NEUROSPORA HYPHAE , 1959 .

[37]  C. R. Thomas.  Image analysis: putting filamentous microorganisms in the picture. , 1992, Trends in biotechnology.