Relaxin receptor in the rat myometrium: regulation by estrogen and relaxin.

The concentration and affinity of the receptors for relaxin in the myometrium of rats were compared during the estrous cycle, in pregnancy, after ovariectomy, and after estrogen and/or relaxin administration. The concentration of the myometrial relaxin receptors in cyclic rats appeared to be related to plasma estrogen levels. Ovariectomy of cyclic animals resulted in the disappearance of receptors by 7 days after surgery. Recovery of the receptors was demonstrable upon estrogen administration to these animals. The response of the uterus to estrogen was biphasic and dependent on the time of exposure to the hormone in vivo. Relaxin receptor concentration increased 5- and 30-fold relative to those at estrus and proestrus within 24 h of the administration of 5 and 200 μg of the steroid, respectively, with no significant change in the receptor's affinity constant. The number of myometrial receptors found in pregnancy were unrelated to the known changes in plasma estrogen levels. However, during late pregnancy,...

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