Gold nanoparticles as novel label for DNA diagnostics

The growing interest in DNA diagnostics, especially in combination with the need for highly-paralleled and miniaturized hybridization assays, is today addressed by fluorescence DNA chips. Fluorescence detection is approved and highly developed, however, it has also problematic aspects, e.g., the low stability of the dyes, the influence of the physicochemical environment onto the signal intensity and the expensive set-up for detection. A novel detection scheme based on metal nanoparticles was proposed to overcome these problems and is discussed in this review.

[1]  J. Storhoff,et al.  A DNA-based method for rationally assembling nanoparticles into macroscopic materials , 1996, Nature.

[2]  P. Schultz,et al.  Organization of 'nanocrystal molecules' using DNA , 1996, Nature.

[3]  C. Mirkin,et al.  Scanometric DNA array detection with nanoparticle probes. , 2000, Science.

[4]  C. Niemeyer,et al.  Site-selective immobilization of gold nanoparticles functionalized with DNA oligomers , 2001 .

[5]  J. Yguerabide,et al.  Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications. , 1998, Analytical biochemistry.

[6]  W. Fritzsche,et al.  Electrical Classification of the Concentration of Bioconjugated Metal Colloids after Surface Adsorption and Silver Enhancement , 2001 .

[7]  Itamar Willner,et al.  Dendritic amplification of DNA analysis by oligonucleotide-functionalized Au-nanoparticles , 2000 .

[8]  J. Köhler,et al.  DNA monolayer on gold substrates characterized by nanoparticle labeling and scanning force microscopy. , 2001, Nucleic acids research.

[9]  C. Mirkin,et al.  Homogeneous, Nanoparticle-Based Quantitative Colorimetric Detection of Oligonucleotides , 2000 .

[10]  W. Fritzsche.  Scanning force microscopy: A microstructured device for imaging, probing, and manipulation of biomolecules at the nanometer scale , 1999 .

[11]  S. P. Fodor,et al.  Multiplexed biochemical assays with biological chips , 1993, Nature.

[12]  Wolfgang Fritzsche,et al.  Selective labeling of oligonucleotide monolayers by metallic nanobeads for fast optical readout of DNA-chips , 2001 .

[13]  Wolfgang Fritzsche,et al.  Nanoparticle-based optical detection of molecular interactions of DNA-chip technology , 2002, SPIE BiOS.

[14]  M. Sussman,et al.  Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array , 1999, Nature Biotechnology.

[15]  J. Köhler,et al.  DNA probes on chip surfaces studied by scanning force microscopy using specific binding of colloidal gold. , 2000, Nucleic acids research.

[16]  F. G. Zaki.  Principles and Techniques of Electron Microscopy , 1975 .

[17]  C. Niemeyer.  REVIEW Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science , 2022 .

[18]  M. Horisberger,et al.  Colloidal gold : a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. , 1981, Scanning electron microscopy.

[19]  J. Köhler,et al.  Chip-based optical detection of DNA hybridization by means of nanobead labeling. , 2000, Analytical chemistry.

[20]  J. Baldeschwieler,et al.  Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[22]  Robert L. Letsinger,et al.  The DNA-Mediated Formation of Supramolecular Mono- and Multilayered Nanoparticle Structures , 2000 .

[23]  Chad A. Mirkin,et al.  One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes , 1998 .

[24]  G. Hacker.  10 – Silver-Enhanced Colloidal Gold for Light Microscopy , 1989 .

[25]  David A. Schultz,et al.  Single-target molecule detection with nonbleaching multicolor optical immunolabels. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[26]  B. Martin,et al.  DNA‐Directed Assembly of Gold Nanowires on Complementary Surfaces , 2001 .