Fluorescent polymeric micelles with aggregation-induced emission properties for monitoring the encapsulation of doxorubicin.

A new type of fluorescent polymeric micelles is developed by self-assembly from a series of amphiphilic block copolymers, poly(ethylene glycol)-b-poly[styrene-co-(2-(1,2,3,4,5-pentaphenyl-1H-silol-1-yloxy)ethyl methacrylate)] [PEG-b-P(S-co-PPSEMA)]. Their capability of loading doxorubicin (DOX) is investigated by monitoring the loading content, encapsulation efficiency, and photophysical properties of micelles. Förster resonance energy transfer from PPSEMA to DOX is observed in DOX-loaded micelles, which can serve as an indication of successful encapsulation of DOX in these micelles. The application of this new type of fluorescent polymeric micelles as a fluorescent probe and an anticancer drug carrier simultaneously is explored by studying the intracellular uptake of DOX-loaded micelles.

[1]  Zhiyuan Zhong,et al.  Reduction-responsive disassemblable core-cross-linked micelles based on poly(ethylene glycol)-b-poly(N-2-hydroxypropyl methacrylamide)-lipoic acid conjugates for triggered intracellular anticancer drug release. , 2012, Biomacromolecules.

[2]  P. Lai,et al.  Improved photodynamic cancer treatment by folate-conjugated polymeric micelles in a KB xenografted animal model. , 2012, Small.

[3]  Kemin Wang,et al.  Single nanoparticle imaging and characterization of different phospholipid-encapsulated quantum dot micelles. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[4]  J. Hao,et al.  Thermally controlled release of anticancer drug from self-assembled γ-substituted amphiphilic poly(ε-caprolactone) micellar nanoparticles. , 2012, Biomacromolecules.

[5]  Yitao Wang,et al.  Polymeric micelles drug delivery system in oncology. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[6]  D. Meldrum,et al.  Using fluorine-containing amphiphilic random copolymers to manipulate the quantum yields of aggregation-induced emission fluorophores in aqueous solutions and the use of these polymers for fluorescent bioimaging. , 2012, Journal of materials chemistry.

[7]  Benedict Law,et al.  Development of biocompatible polymeric nanoparticles for in vivo NIR and FRET imaging. , 2012, Bioconjugate chemistry.

[8]  D. Meldrum,et al.  A series of poly[N-(2-hydroxypropyl)methacrylamide] copolymers with anthracene-derived fluorophores showing aggregation-induced emission properties for bioimaging. , 2012, Journal of polymer science. Part A, Polymer chemistry.

[9]  Ben Zhong Tang,et al.  Biocompatible Nanoparticles with Aggregation‐Induced Emission Characteristics as Far‐Red/Near‐Infrared Fluorescent Bioprobes for In Vitro and In Vivo Imaging Applications , 2012 .

[10]  C. Tsitsilianis,et al.  Self-assembly and drug delivery studies of pH/thermo-sensitive polyampholytic (A-co-B)-b-C-b-(A-co-B) segmented terpolymers , 2011 .

[11]  Afsaneh Lavasanifar,et al.  Engineering of amphiphilic block copolymers for polymeric micellar drug and gene delivery. , 2011, Journal of controlled release : official journal of the Controlled Release Society.

[12]  Ben Zhong Tang,et al.  Aggregation-induced emission. , 2011, Chemical Society reviews.

[13]  D. Meldrum,et al.  Micelles as Delivery Vehicles for Oligofluorene for Bioimaging , 2011, PloS one.

[14]  Jean-François Gohy,et al.  Photo-induced micellization of block copolymers bearing 4,5-dimethoxy-2-nitrobenzyl side groups , 2011 .

[15]  Ho-Chul Shin,et al.  A 3-in-1 polymeric micelle nanocontainer for poorly water-soluble drugs. , 2011, Molecular pharmaceutics.

[16]  R. Jain,et al.  Multistage nanoparticle delivery system for deep penetration into tumor tissue , 2011, Proceedings of the National Academy of Sciences.

[17]  Gert Storm,et al.  Polymeric Micelles in Anticancer Therapy: Targeting, Imaging and Triggered Release , 2010, Pharmaceutical Research.

[18]  D. S. Lee,et al.  An acidic pH-triggered polymeric micelle for dual-modality MR and optical imaging , 2010 .

[19]  A. Jen,et al.  Enhancement of Aggregation‐Induced Emission in Dye‐Encapsulating Polymeric Micelles for Bioimaging , 2010 .

[20]  D. Meldrum,et al.  2,1,3-Benzothiadiazole (BTD)-moiety-containing red emitter conjugated amphiphilic poly(ethylene glycol)-block-poly(epsilon-caprolactone) copolymers for bioimaging. , 2010, Journal of materials chemistry.

[21]  C. Allen,et al.  Block copolymer micelles for delivery of cancer therapy: transport at the whole body, tissue and cellular levels. , 2009, Journal of controlled release : official journal of the Controlled Release Society.

[22]  F. Szoka,et al.  Soluble polymer carriers for the treatment of cancer: the importance of molecular architecture. , 2009, Accounts of chemical research.

[23]  B. Tang,et al.  Aggregation-induced emission: phenomenon, mechanism and applications. , 2009, Chemical Communications.

[24]  A. Whittaker,et al.  Synthesis and evaluation of partly fluorinated block copolymers as MRI imaging agents. , 2009, Biomacromolecules.

[25]  Ick Chan Kwon,et al.  Activatable imaging probes with amplified fluorescent signals. , 2008, Chemical communications.

[26]  Kinam Park,et al.  Release of hydrophobic molecules from polymer micelles into cell membranes revealed by Förster resonance energy transfer imaging , 2008, Proceedings of the National Academy of Sciences.

[27]  Eric D. Pressly,et al.  Synthesis and characterization of core-shell star copolymers for in vivo PET imaging applications. , 2008, Biomacromolecules.

[28]  Bin Zhao,et al.  Multiple Micellization and Dissociation Transitions of Thermo- and Light-Sensitive Poly(ethylene oxide)-b-poly(ethoxytri(ethylene glycol) acrylate-co-o-nitrobenzyl acrylate) in Water , 2008 .

[29]  Kwangmeyung Kim,et al.  Polymers for bioimaging , 2007 .

[30]  Robert Langer,et al.  Formulation of functionalized PLGA-PEG nanoparticles for in vivo targeted drug delivery. , 2007, Biomaterials.

[31]  Igor L. Medintz,et al.  Materials for fluorescence resonance energy transfer analysis: beyond traditional donor-acceptor combinations. , 2006, Angewandte Chemie.

[32]  D. Maysinger,et al.  Block copolymer micelles as delivery vehicles of hydrophobic drugs: Micelle–cell interactions , 2006, Journal of drug targeting.

[33]  Marie-Hélène Dufresne,et al.  Block copolymer micelles: preparation, characterization and application in drug delivery. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[34]  Ben Zhong Tang,et al.  Synthesis, Light Emission, Nanoaggregation, and Restricted Intramolecular Rotation of 1,1-Substituted 2,3,4,5-Tetraphenylsiloles , 2003 .

[35]  Kazuhito Watanabe,et al.  Synthesis, nanostructures, and functionality of amphiphilic liquid crystalline block copolymers with azobenzene moieties , 2002 .

[36]  Daoben Zhu,et al.  Efficient blue emission from siloles , 2001 .

[37]  Y. Hsieh,et al.  Wetting and absorbency of nonionic surfactant solutions on cotton fabrics , 2001 .

[38]  H. Maeda,et al.  Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review. , 2000, Journal of controlled release : official journal of the Controlled Release Society.

[39]  Jamshidi,et al.  Conjugated 1,10-Phenanthrolines as Tunable Fluorophores. , 1999, Angewandte Chemie.

[40]  J. K. Thomas,et al.  Environmental effects on vibronic band intensities in pyrene monomer fluorescence and their application in studies of micellar systems , 1977 .

[41]  W. Melhuish,et al.  QUANTUM EFFICIENCIES OF FLUORESCENCE OF ORGANIC SUBSTANCES: EFFECT OF SOLVENT AND CONCENTRATION OF THE FLUORESCENT SOLUTE1 , 1961 .

[42]  Ick Chan Kwon,et al.  Polymeric nanomedicine for cancer therapy , 2008 .