Morphological and Histochemical Investigation on Glandular Trichomes of Orobanche ramosa subsp. nana (Orobanchaceae)

Germany summary. Glandular trichomes present in the stems and flower parts of Orobanche ramosa L. subsp. nana (REUTER) COUTINHO were examined under conventional microscopy, UV microscopy and scanning electron microscopy. The ontogenesis and morphology of the glandular trichomes appeared to be identical to those reported for other species belonging to Geraniaceae, Solanaceae. Cannabaceae and Scrophulariaceae. Histochemical investigations were performed to qualitatively detect the main classes of compounds occurring in the glandular hairs and in the secretion. The results achieved suggested the presence of terpenes and flavonoids as the main chemicals. The absence of positive reactions to sesquiterpenes contrasts with similar previously written papers which report sesquiterpenes, together with phenylpropanoids and iridoid glycosides as characteristic chemicals of the genus.

[1]  M. Nicoletti,et al.  GLANDULAR TRICHOMES OF CALCEOLARIA ADSCENDENS LIDL. (SCROPHULARIACEAE): HISTOCHEMISTRY, DEVELOPMENT AND ULTRASTRUCTURE , 1999 .

[2]  L. Ascensão,et al.  The Leaf Capitate Trichomes ofLeonotis leonurus: Histochemistry, Ultrastructure and Secretion , 1998 .

[3]  A. Bisio,et al.  Structural and Histochemical Investigation of the Glandular Trichomes of Salvia aurea L. Leaves, and Chemical Analysis of the Essential Oil , 1997 .

[4]  M. Nicoletti,et al.  Development and morphology of secretory trichomes of Calceolaria volckmanni (Scrophulariaceae) , 1996 .

[5]  Prof. Robert Hegnauer Chemotaxonomie der Pflanzen , 1996, Lehrbücher und Monographien aus dem Gebiete der Exakten Wissenschaften.

[6]  M. Serafini,et al.  The occurrence of phenylpropanoid glycosides in Italian Orobanche spp. , 1995 .

[7]  J. Garbarino,et al.  THE DISTRIBUTION OF PHENYLPROPANOID GLYCOSIDES IN CHILEAN CALCEOLARIA SPP. , 1995 .

[8]  A. Bruni,et al.  Histological Localization of Coumarins in Different Organs of Smyrnium perfoliatum (Apiaceae) , 1995 .

[9]  E. Werker Function of essential oil‐secreting glandular hairs in aromatic plans of Lamiacea—a review , 1993 .

[10]  J. Harborne Quimica de la flora de Chile : edited by O. Munoz, Department of Technical Investigation, University of Chile, 1992. 351 pp. Price unknown. ISBN 956-19-0174-9. , 1993 .

[11]  O. Sticher,et al.  2'-O-acetylpoliumoside: a new phenylpropanoid glycoside from Orobanche ramosa. , 1989, Planta medica.

[12]  J. Garbarino,et al.  Foliosate, a bis-diterpene and 9-epi-ent-7,15-isopimaradiene· derivatives from Calceolaria foliosa , 1989 .

[13]  E. Wollenweber,et al.  Flavonoid aglycones excreted by three Calceolaria species , 1989 .

[14]  J. Garbarino,et al.  Polar Constituents of Calceolaria ascendens1. , 1988, Planta medica.

[15]  A. Fahn Secretory tissues in vascular plants. , 1988, The New phytologist.

[16]  A. Bruni,et al.  Morphology and secretion of glandular trichomes in Tamus communis , 1987 .

[17]  Peter B. Gahan,et al.  Plant histochemistry and cytochemistry : an introduction , 1984 .

[18]  William A. Jensen,et al.  Botanical histochemistry : principles and practice , 1962 .