Oxidative stress induced by 1.8 GHz radio frequency electromagnetic radiation and effects of garlic extract in rats

Abstract Purpose: We aimed to study the oxidative damage induced by radiofrequency electromagnetic radiation (RF-EMR) emitted by mobile telephones and the protective effect of garlic extract used as an anti-oxidant against this damage. Materials and methods: A total of 66 albino Wistar rats were divided into three groups. The first group of rats was given 1.8 GHz, 0.4 W/kg specific absorption rate (SAR) for 1 h a day for three weeks. The second group was given 500 mg/kg garlic extract in addition to RF-EMR. The third group of rats was used as the control group. At the end of the study, blood and brain tissue samples were collected from the rats. Results: After the RF-EMR exposed, the advanced oxidation protein product (AOPP) levels of brain tissue increased compared with the control group (p < 0.001). Garlic administration accompanying the RF-EMR, on the other hand, significantly reduced AOPP levels in brain tissue (p < 0.001). The serum nitric oxide (NO) levels significantly increased both in the first and second group (p < 0.001). However, in the group for which garlic administration accompanied that of RF-EMR, there was no difference in serum NO levels compared with the RF-EMR exposed group (p > 0.05). There was no significant difference among the groups with respect to malondialdehyde (MDA) levels in brain tissue and blood samples (p > 0.05). Similarly, no difference was detected among the groups regarding serum paroxonase (PON) levels (p > 0.05). We did not detect any PON levels in the brain tissue. Conclusions: The exposure of RF-EMR similar to 1.8 GHz Global system for mobile communication (GSM) leads to protein oxidation in brain tissue and an increase in serum NO. We observed that garlic administration reduced protein oxidation in brain tissue and that it did not have any effects on serum NO levels.

[1]  Fehmi Ozguner,et al.  900 MHz radiofrequency-induced histopathologic changes and oxidative stress in rat endometrium: protection by vitamins E and C , 2007, Toxicology and industrial health.

[2]  H. Ferreira,et al.  Ultra high frequency-electromagnetic field irradiation during pregnancy leads to an increase in erythrocytes micronuclei incidence in rat offspring. , 2006, Life sciences.

[3]  L. Challis,et al.  Mechanisms for interaction between RF fields and biological tissue , 2005, Bioelectromagnetics.

[4]  W. Dröge.  Aging-related changes in the thiol/disulfide redox state: implications for the use of thiol antioxidants , 2002, Experimental Gerontology.

[5]  N. Delibaş,et al.  PROTECTIVE EFFECTS OF VITAMINS C AND E AGAINST ENDOMETRIAL DAMAGE AND OXIDATIVE STRESS IN FLUORIDE INTOXICATION , 2007, Clinical and experimental pharmacology & physiology.

[6]  T. Jay,et al.  Effect of Exposure to 1,800 MHz Electromagnetic Fields on Heat Shock Proteins and Glial Cells in the Brain of Developing Rats , 2011, Neurotoxicity Research.

[7]  M. Protopopova,et al.  915 MHz microwaves and 50 Hz magnetic field affect chromatin conformation and 53BP1 foci in human lymphocytes from hypersensitive and healthy persons , 2005, Bioelectromagnetics.

[8]  Z. Somosy,et al.  Radiation response of cell organelles. , 2000, Micron.

[9]  Györgyi Kubinyi,et al.  Effect of whole-body 1800MHz GSM-like microwave exposure on testicular steroidogenesis and histology in mice. , 2006, Reproductive toxicology.

[10]  N. Seyhan,et al.  Mobile phone radiation-induced free radical damage in the liver is inhibited by the antioxidants n-acetyl cysteine and epigallocatechin-gallate , 2010, International journal of radiation biology.

[11]  Rony Seger,et al.  Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies. , 2007, The Biochemical journal.

[12]  Maria Blettner, Gabriele Berg.  Are Mobile Phones Harmful? , 2000, Acta oncologica.

[13]  Fehmi Ozguner,et al.  Oxidative Stress‐Mediated Skin Damage in an Experimental Mobile Phone Model Can Be Prevented by Melatonin , 2004, The Journal of dermatology.

[14]  Suleyman Dasdag,et al.  Whole body exposure of rats to microwaves emitted from a cell phone does not affect the testes , 2003, Bioelectromagnetics.

[15]  A. Armağan,et al.  A novel antioxidant agent caffeic acid phenethyl ester prevents shock wave-induced renal tubular oxidative stress , 2005, Urological Research.

[16]  I. Meral,et al.  Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs , 2007, Brain Research.

[17]  Erdinç Devrim,et al.  Effects of mobile phone use on brain tissue from the rat and a possible protective role of vitamin C – a preliminary study , 2010, International journal of radiation biology.

[18]  J. Berliner,et al.  Protective effect of high density lipoprotein associated paraoxonase. Inhibition of the biological activity of minimally oxidized low density lipoprotein. , 1995, The Journal of clinical investigation.

[19]  G. Kızıl,et al.  Effect of 900 MHz Radio Frequency Radiation on Beta Amyloid Protein, Protein Carbonyl, and Malondialdehyde in the Brain , 2012, Electromagnetic biology and medicine.

[20]  Ömer Akyol,et al.  Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits , 2002, Cell biochemistry and function.

[21]  H. Esterbauer,et al.  Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. , 1991, Free radical biology & medicine.

[22]  C. Borek,et al.  Antioxidant health effects of aged garlic extract. , 2001, The Journal of nutrition.

[23]  Fehmi Ozguner,et al.  Melatonin modulates 900 MHz microwave-induced lipid peroxidation changes in rat brain , 2006, Toxicology and industrial health.

[24]  Selcuk Comlekci,et al.  Endometrial apoptosis induced by a 900-MHz mobile phone: Preventive effects of vitamins E and C , 2006, Advances in therapy.

[25]  S. Kamışlı,et al.  Ginkgo biloba prevents mobile phone-induced oxidative stress in rat brain. , 2004, Clinica chimica acta; international journal of clinical chemistry.

[26]  L. Packer,et al.  Oxidative damage to proteins: spectrophotometric method for carbonyl assay. , 1994, Methods in enzymology.

[27]  D. Leibfritz,et al.  Free radicals and antioxidants in normal physiological functions and human disease. , 2007, The international journal of biochemistry & cell biology.

[28]  R. Adair,et al.  Biophysical limits on athermal effects of RF and microwave radiation , 2003, Bioelectromagnetics.

[29]  S. Karakucuk,et al.  Serum Paraoxonase 1 Activity and Lipid Peroxidation Levels in Patients with Age-Related Macular Degeneration , 2005, Ophthalmologica.

[30]  Ali Ihsan Yurekli,et al.  GSM Base Station Electromagnetic Radiation and Oxidative Stress in Rats , 2006, Electromagnetic biology and medicine.

[31]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[32]  G. Take,et al.  Effect of fluoride intoxication on endometrial apoptosis and lipid peroxidation in rats: role of vitamins E and C. , 2007, Toxicology.

[33]  A. Hausladen,et al.  Nitrosative stress. , 1999, Methods in enzymology.

[34]  E. Ulukaya,et al.  Mobile phone exposure does not induce apoptosis on spermatogenesis in rats. , 2008, Archives of medical research.

[35]  James B. Mitchell,et al.  Nitric oxide and cancer: an introduction. , 2003, Free radical biology & medicine.

[36]  B. La Du,et al.  Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase allozymes Q and R. , 1998, Arteriosclerosis, thrombosis, and vascular biology.

[37]  C. S. Cooper,et al.  The chemistry of lipid peroxidation metabolites: Crosslinking reactions of malondialdehyde , 2006, Lipids.

[38]  B. Halliwell,et al.  Free radicals and metal ions in health and disease , 1987, Proceedings of the Nutrition Society.

[39]  C. Timmel,et al.  The effects of weak magnetic fields on radical recombination reactions in micelles , 2000, International journal of radiation biology.

[40]  B. Halliwell,et al.  Free Radicals and Hearing: Cause, Consequence, and Criteria , 1999, Annals of the New York Academy of Sciences.

[41]  Dusan Sokolovic,et al.  Melatonin reduces oxidative stress induced by chronic exposure of microwave radiation from mobile phones in rat brain. , 2008, Journal of radiation research.

[42]  S. C. Manchanda,et al.  Chronic garlic administration protects rat heart against oxidative stress induced by ischemic reperfusion injury , 2002, BMC pharmacology.

[43]  S. Ghavami,et al.  Association of L55M and Q192R polymorphisms of paraoxonase-1 gene with preeclampsia. , 2011, Archives of medical research.

[44]  K J Rothman,et al.  Overall mortality of cellular telephone customers. , 1996, Epidemiology.

[45]  G. Kismali,et al.  The influence of 1800 MHz GSM-like signals on blood chemistry and oxidative stress in non-pregnant and pregnant rabbits , 2012, International journal of radiation biology.

[46]  F. Dal-Pizzol,et al.  Oxidative stress effects on the central nervous system of rats after acute exposure to ultra high frequency electromagnetic fields , 2006, Bioelectromagnetics.

[47]  Fehmi Ozguner,et al.  A novel antioxidant agent caffeic acid phenethyl ester prevents long-term mobile phone exposure-induced renal impairment in rat , 2005, Molecular and Cellular Biochemistry.