Heterodyne interferometric surface plasmon resonance biosensor

A high-sensitivity surface plasmon resonance (SPR) biosensor is constructed by using a common-path heterodyne interferometric system. The beam combiner is employed to combine the modulated He-Ne TE-wave and TM-wave into a heterodyne light source with a frequency difference of 60 kHz. Using the common-path heterodyne interferometric system to obtain the phase shift between the two waves has the advantage of high-sensitivity real-time phase detection for monitoring molecular interactions. By detecting the phase shift, the proposed SPR biosensor is employed to monitor the interactions between the sheep IgG covalently immobilized on the sensor chip surface and the anti-sheep IgG contained in the running buffer.

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