Insect Epicuticular Grease Visualised by Scanning Probe Microscopy

The covering wings (elytra) in the Colorado potato beetle Leptinotarsa decemlineata appear shiny, smooth, water-repellent, and slightly slippery, (Fig. 1). These properties are due to the presence of epicuticle, the outermost layer of the insect integument, covered by a wax-like lipid surface layer called grease. Surface waxes have been previously reported in a variety of conditions, from liquid viscous coatings to crystalline structures in form of plates, rods and filaments from many insects (adults and larvae) and arachnids (Hadley, 1981a,b). Beament (1945) and Wigglesworth (1945) considered an outer thin layer of solid wax, whereas Lewis (1962) rather assumed an oil film on the epicuticle surface to be widespread throughout insects.

[1]  Vladimir V Tsukruk,et al.  Viscoelastic nanoscale properties of cuticle contribute to the high-pass properties of spider vibration receptor (Cupiennius salei Keys) , 2007, Journal of The Royal Society Interface.

[2]  S. Gorb.  Fly Microdroplets Viewed Big: a Cryo-SEM Approach , 2006, Microscopy Today.

[3]  Stanislav Gorb,et al.  Adhesion forces measured at the level of a terminal plate of the fly's seta , 2004, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[4]  U. Schwarz,et al.  Chemical composition of the attachment pad secretion of the locust Locusta migratoria. , 2002, Insect biochemistry and molecular biology.

[5]  N. F. Hadley.  CUTICULAR LIPIDS OF TERRESTRIAL PLANTS AND ARTHROPODS: A COMPARISON OF THEIR STRUCTURE, COMPOSITION, AND WATERPROOFING FUNCTION , 1981 .

[6]  Dr. Anthony C. Neville.  Biology of the Arthropod Cuticle , 1975, Zoophysiology and Ecology.

[7]  A. Gilby,et al.  The cuticular lipids of the cockroach, Periplaneta americana (L.) , 1963 .

[8]  C. T. Lewis.  Diffusion of Oil Films over Insects , 1962, Nature.

[9]  J. Beament.  WAX SECRETION IN THE COCKROACH , 1955 .

[10]  L. Wolfe.  Studies of the Development of the Imaginal Cuticle of Calliphora erythrocephala , 1954 .

[11]  V. Wigglesworth,et al.  THE INSECT CUTICLE , 1948, Biological reviews of the Cambridge Philosophical Society.

[12]  A. D. Lees,et al.  An egg-waxing organ in ticks. , 1948, The Quarterly journal of microscopical science.

[13]  J. Beament.  The cuticular lipoids of insects , 1945 .

[14]  J. A. Ramsay.  The Evaporation of Water from the Cockroach , 1935 .

[15]  M. Scherge,et al.  Biological micro- and nanotribology , 2001 .

[16]  E. Bernays,et al.  Plant and insect cuticular lipids serve as behavioral cues for insects , 1991 .

[17]  G. Hendricks,et al.  Structure of the cuticle of the common house cricket with reference to the location of lipids. , 1983, Tissue & cell.

[18]  N. F. Hadley.  Fine structure of the cuticle of the black widow spider with reference to surface lipids. , 1981, Tissue & cell.

[19]  Michael Locke,et al.  Chapter 2 – THE STRUCTURE AND FORMATION OF THE INTEGUMENT IN INSECTS , 1974 .

[20]  A. Richards.  The Integument of Arthropods : the chemical Components and their Properties, the Anatomy and Development, and the Permeability. , 1951 .