Chemiluminescent aptasensor for chloramphenicol based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized flower-like gold nanostructures and magnetic nanoparticles

A novel chemiluminescent aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed using biotinylated CAP aptamer-functionalized magnetic nanoparticles (MNPs) as capture probes and thiolated hybridized complementary strand-modified N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized flower-like gold nanostructures (AuNFs) as signal probes. P-iodophenol (PIP) was also added to form an ABEI-H2O2-PIP steady-state chemiluminescence (CL) system. Based on a competitive format, the CL intensity was negatively correlated with the concentration of CAP in the range of 0.01–0.20 ng/mL and the detection limit was 0.01 ng/mL in buffer and 1 ng/mL in milk. The proposed method was successfully applied to measure CAP in milk samples and compared to a commercial ELISA method. The high sensitivity of AuNFs, excellent selectivity and stability of aptamers, and good overall stability of the chemiluminescent bioassay with magnetic separation make them a promising approach for the detection of small molecular illegal additives. Additionally, the high sensitivity, easy operation, and good reproducibility exhibited by the stable chemiluminescent bioassay demonstrate its applicability for the trace detection of CAP in applications, such as animal husbandry.

[1]  Shi-quan Han,et al.  Chemiluminescence immunoassay for chloramphenicol , 2005, Analytical and bioanalytical chemistry.

[2]  Ke Wang,et al.  Development of a homogeneous immunoassay based on the AlphaLISA method for the detection of chloramphenicol in milk, honey and eggs. , 2012, Journal of the science of food and agriculture.

[3]  H. Cui,et al.  Chitosan-Luminol Reduced Gold Nanoflowers: From One-Pot Synthesis to Morphology-Dependent SPR and Chemiluminescence Sensing , 2008 .

[4]  Ke-Jing Huang,et al.  A luminol electrochemiluminescence aptasensor based on glucose oxidase modified gold nanoparticles for measurement of platelet-derived growth factor BB. , 2015, Talanta.

[5]  Paolo Bertoncello,et al.  Nanostructured materials for electrochemiluminescence (ECL)-based detection methods: recent advances and future perspectives. , 2009, Biosensors & bioelectronics.

[6]  M. J. Kasten.  Clindamycin, metronidazole, and chloramphenicol. , 1999, Mayo Clinic proceedings.

[7]  G. Whitesides,et al.  SELECTIVE REDUCTION OF DISULFIDES BY TRIS(2-CARBOXYETHYL) PHOSPHINE , 1991 .

[8]  Tarasankar Pal,et al.  Interparticle coupling effect on the surface plasmon resonance of gold nanoparticles: from theory to applications. , 2007, Chemical reviews.

[9]  G. Patonay,et al.  Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry. , 1988, Analytical chemistry.

[10]  Hua Cui,et al.  A competitive immunoassay for sensitive detection of small molecules chloramphenicol based on luminol functionalized silver nanoprobe. , 2014, Analytica chimica acta.

[11]  Tanyu Wang,et al.  Quantitation of femtomolar protein levels via direct readout with the electrochemical proximity assay. , 2012, Journal of the American Chemical Society.

[12]  Fang Zhang,et al.  One-pot synthesis and bioapplication of amine-functionalized magnetite nanoparticles and hollow nanospheres. , 2006, Chemistry.

[13]  C. Xie,et al.  Determination of tannic acid in industrial wastewater based on chemiluminescence system of KIO₄-H₂O₂-Tween40. , 2010, Luminescence : the journal of biological and chemical luminescence.

[14]  Ronny Blust,et al.  An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol , 2014, Sensors.

[15]  Wen-Chang Shen,et al.  Nanoparticle-based electrochemiluminescence immunosensor with enhanced sensitivity for cardiac troponin I using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles as labels. , 2011, Biosensors & bioelectronics.

[16]  T. Shibata,et al.  Facile Assay of Telomerase Activity Utilizing a DNA-detectable Chemiluminogenic Reagent , 2008, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[17]  Rongxiu Li,et al.  Molecularly imprinted polymer microspheres for solid-phase extraction of chloramphenicol residues in foods. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[18]  L. Kricka,et al.  Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol. , 1996, Analytical biochemistry.

[19]  M. Scippo,et al.  In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. , 2011, Journal of biotechnology.

[20]  H. Luo,et al.  Post-chemiluminescence determination of chloramphenicol based on luminol-potassium periodate system. , 2012, Luminescence : the journal of biological and chemical luminescence.

[21]  Loïc J Blum,et al.  Applications of the luminol chemiluminescent reaction in analytical chemistry , 2006, Analytical and bioanalytical chemistry.

[22]  Jeanne V. Samsonova,et al.  Competitive ELISA of Chloramphenicol: Influence of Immunoreagent Structure and Application of the Method for the Inspection of Food of Animal Origin , 2000 .

[23]  C. J. Easley,et al.  Improvement of sensitivity and dynamic range in proximity ligation assays by asymmetric connector hybridization. , 2010, Analytical chemistry.

[24]  Zhouping Wang,et al.  Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles , 2015 .

[25]  C. Shannon,et al.  A Reusable Electrochemical Proximity Assay for Highly Selective, Real-Time Protein Quantitation in Biological Matrices , 2014, Journal of the American Chemical Society.

[26]  Xin Wang,et al.  In situ cell for electrochemically generated Ru(bpy)33+-based chemiluminescence detection in capillary electrophoresis , 1999 .

[27]  Xu Hun,et al.  Functionalized fluorescent core-shell nanoparticles used as a fluorescent labels in fluoroimmunoassay for IL-6. , 2007, Biosensors & bioelectronics.

[28]  Zhouping Wang,et al.  Electrochemiluminescent aptamer biosensor for the determination of ochratoxin A at a gold-nanoparticles-modified gold electrode using N-(aminobutyl)-N-ethylisoluminol as a luminescent label , 2010, Analytical and bioanalytical chemistry.

[29]  Xiluan Yan,et al.  Label-free aptamer-based chemiluminescence detection of adenosine. , 2009, Talanta.

[30]  Freddy Dardenne,et al.  An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine , 2015, Sensors.

[31]  H. Cui,et al.  A label-free electrochemiluminescence aptasensor for thrombin based on novel assembly strategy of oligonucleotide and luminol functionalized gold nanoparticles. , 2013, Biosensors & bioelectronics.

[32]  H. Nakano,et al.  New phenylboronic acid derivatives as enhancers of the luminol-H(2)O(2)-horseradish peroxidase chemiluminescence reaction. , 1999, Luminescence : the journal of biological and chemical luminescence.

[33]  Da Xing,et al.  PCR-free quantitative detection of genetically modified organism from raw materials. An electrochemiluminescence-based bio bar code method. , 2008, Analytical chemistry.

[34]  Anatoly V. Zherdev,et al.  Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk , 2013, Analytical and Bioanalytical Chemistry.

[35]  Kevin N. Andrew,et al.  Analytical applications of flow injection with chemiluminescence detection--a review. , 2001, Luminescence : the journal of biological and chemical luminescence.

[36]  B. Bartolo,et al.  Fluorescence studies of the Er ↔ Ho and Er ↔ Tm energy transfer in erbium aluminum garnet , 1978 .

[37]  I. Shalit,et al.  Chloramphenicol in the 1980s , 1984, Drugs.

[38]  Wen-Chang Shen,et al.  Highly chemiluminescent gold nanopopcorns functionalized by N-(aminobutyl)-N-(ethylisoluminol) with lipoic acid as a co-stabilizing reagent. , 2013, Journal of materials chemistry. B.

[39]  Yongxin Li,et al.  Development of a novel luminol chemiluminescent method catalyzed by gold nanoparticles for determination of estrogens , 2007, Analytical and bioanalytical chemistry.

[40]  P. He,et al.  Study on the electrochemiluminescence behavior of ABEI and its application in DNA hybridization analysis. , 2002, The Analyst.