The toxin from Gymnodinium veneficum Ballantine

Methods of extraction of the toxin from cultures of Gymnodinium veneficum are described. This is now done by dialysis and evaporation under reduced pressure. The toxin molecule must be large, as it cannot penetrate a dialysis membrane; it is soluble in water and the lower alcohols, but insoluble in ether and chloroform. It is unstable in acids, turning into another toxic product, and is decomposed by hot alkali, though in neutral solution is more or less thermostable. The toxin as it occurs in sea water is not the same as paralytic shellfish poison, but there are some resemblances between this and the 'acid extract', though much more work is needed to check this point. The action of the toxin on a variety of animals is described, and an attempt is made to devise an approximate assay technique using gobies as the test animals. The action has also been observed on a range of isolated preparations, and although the final conclusion as to the mode of action is not quite clear, we feel confident that the site of action in whole animals is in the nervous system, probably acting on ganglion transmission. With regard to the mode of action it depolarizes nerve and muscle membranes. It also abolishes the potential across frog skin without measurably altering the skin resistance. This depolarization probably occurs by interference with the sodium exchange mechanism, allowing rapid entry of sodium into the cells.