Application of rumen microorganisms for enhanced anaerobic fermentation of corn stover.

Anaerobic fermentation of corn stover by rumen microorganisms in both batch and semi-continuous cultures was studied. In the batch reactors, a high volatile solids (VS) conversion efficiency of 65‐70% was achieved after 240 h incubation at 25‐40 8C. The volatile fatty acids (VFA) yield ranged from 0.59 to 0.71 g g � 1 VS utilized. Acetate, propionate and butyrate were the main aqueous products with small amounts of i-butyrate and valerate also being produced. Biogas was composed of methane and carbon dioxide. In the semi-continuous reactor, at loading rates of 10‐30 g VS l � 1 d � 1 and 40 8C, a VS conversion efficiency of 65% was achieved as a solids and hydraulic retention time of 96 and 18 h, respectively. The VFA yield varied between 0.56 and 0.59 g g � 1 VS utilized. Biogas was composed of hydrogen and carbon dioxide, but was free of methane. The experimental results demonstrate that the anaerobic fermentation of corn stover by rumen microorganisms was able to rapidly degrade the volatile solids and produce useful VFAs with high yields. # 2004 Elsevier Ltd. All rights reserved.

[1]  A. Kivaisi,et al.  Effects of lignin on the anaerobic degradation of (ligno) cellulosic wastes by rumen microorganisms , 1988, Applied Microbiology and Biotechnology.

[2]  A. Kivaisi,et al.  Application of rumen microorganisms for enhanced anaerobic degradation of bagasse and maize bran , 1995 .

[3]  Richard R. Davison,et al.  Biomass conversion to mixed alcohol fuels using the MixAlco process , 1999, Applied biochemistry and biotechnology.

[4]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[5]  H J Gijzen,et al.  Conversion of cereal residues into biogas in a rumen-derived process , 1992, World journal of microbiology & biotechnology.

[6]  Bert Hamelers,et al.  Effect of temperature on hydrolysis rates of selected biowaste components , 1999 .

[7]  R. Datta.  Acidogenic fermentation of corn stover , 1981 .

[8]  M. Holtzapple,et al.  Fermentation of corn stover to carboxylic acids. , 2003, Biotechnology and bioengineering.

[9]  H. Gijzen,et al.  Continuous cultivation of rumen microorganisms, a system with possible application to the anaerobic degradation of lignocellulosic waste materials , 1986, Applied Microbiology and Biotechnology.

[10]  T. Noike,et al.  Characteristics of carbohydrate degradation and the rate‐limiting step in anaerobic digestion , 1985, Biotechnology and bioengineering.

[11]  J. Keller,et al.  Cellulosic waste degradation by rumen-enhanced anaerobic digestion. , 2003, Water science and technology : a journal of the International Association on Water Pollution Research.

[12]  P. Wilderer,et al.  Membrane separation to improve degradation of road side grass by rumen enhanced solid incubation. , 2003, Water science and technology : a journal of the International Association on Water Pollution Research.

[13]  M. Ladisch,et al.  Anaerobic fermentation: Microbes from ruminants , 1985 .

[14]  Michael R. Ladisch,et al.  Process considerations in the enzymatic hydrolysis of biomass , 1983 .

[15]  H. Gijzen,et al.  Anaerobic digestion of cellulose fraction of domestic refuse by means of rumen microorganisms , 1988, Biotechnology and bioengineering.

[16]  T. Wood,et al.  Carbohydrates and carbohydrate esters of ferulic acid released from cell walls of Lolium multiflorum by treatment with cellulolytic enzymes , 1976 .

[17]  L. H. Koehler.  Differentiation of carbohydrates by anthrone reaction rate and color intensity. , 1952 .