Effects of mating on plasma concentrations of testosterone, cortisol, oestrone sulphate and 15-ketodihydro-PGF2alpha in stallions.

Very little information is available regarding the physiological mechanisms involved in the normal sexual activity in the stallion and, in particular, the endocrine control of reproduction is still not clearly understood. This experiment was designed to determine the short-term effect of sexual stimulation on plasma concentrations of testosterone, cortisol, oestrone sulphate and 15-ketodihydro-PGF(2alpha) in stallions. Semen samples were collected from 10 lighthorse stallions of proven fertility using a Missouri model artificial vagina. At the same time, blood samples were collected from the jugular vein with heparinized tubes, 20 and 10 min before oestrous mare exposure, at exposure and 10, 20, 30 min after dismounting. Testosterone concentrations showed a sharp rise 10 min after mating (p < 0.001), reached a plateau, and then showed a further increase 30 min after mating (p < 0.001). Cortisol concentrations increased 10 min after mating (p < 0.001) and remained at high levels in the subsequent samples taken. A peak of oestrone sulphate was observed 10 min after mating (p < 0.001). 15-Ketodihydro-PGF(2alpha) concentrations decreased rapidly at the moment of the exposure of the stallions to an oestrous mare (p < 0.05), returned to pre-mating concentrations and then decreased again 30 min after mating (p < 0.05).

[1]  B. Bilińska,et al.  Expression of aromatase and oestrogen receptors in reproductive tissues of the stallion and a single cryptorchid visualised by means of immunohistochemistry. , 2005, Domestic animal endocrinology.

[2]  H. Kindahl,et al.  The cow in endocrine focus before and after calving. , 2004, Reproduction in domestic animals = Zuchthygiene.

[3]  H. Kindahl,et al.  Tetracosactid (ACTH)treatment of mares in estrus-effect on estrous signs, ovulation and hormone levels , 2002 .

[4]  O. Rampin,et al.  Sexual behavior of stallions during in-hand natural service and semen collection: an observation in French studs. , 2001, Animal reproduction science.

[5]  J. Raeside,et al.  Estrogen concentrations in semen of the stallion. , 1997, Animal reproduction science.

[6]  K. Dowsett,et al.  The influence of age and breed on stallion semen. , 1996, Theriogenology.

[7]  R. Thun,et al.  [Relationship between cortisol and testosterone during resting conditions, after acute stress and hormone stimulation in steers]. , 1996, Schweizer Archiv fur Tierheilkunde.

[8]  B. Kemp,et al.  Reproductive characteristics and semen quality in maiden Dutch Warmblood stallions. , 1994, Journal of reproduction and fertility.

[9]  R. Flower,et al.  Lipocortin-1: cellular mechanisms and clinical relevance. , 1994, Trends in pharmacological sciences.

[10]  R. P. Amann,et al.  Relationship between morphologic and seminal characteristics of equine spermatozoa , 1993 .

[11]  R. Claus,et al.  Estrogens and prostaglandin F2α in the semen and blood plasma of stallions , 1992 .

[12]  K. Borg,et al.  Cortisol, growth hormone, and testosterone concentrations during mating behavior in the bull and boar. , 1991, Journal of animal science.

[13]  D. Thompson,et al.  Responses of cortisol and prolactin to sexual excitement and stress in stallions and geldings. , 1991, Journal of animal science.

[14]  D. Thompson,et al.  Effects of sexual stimulation, with and without ejaculation, on serum concentrations of LH, FSH, testosterone, cortisol and prolactin in stallions. , 1989, Journal of animal science.

[15]  D. Thompson,et al.  Effect of administration of adrenocorticotropic hormone on plasma concentrations of testosterone, luteinizing hormone, follicle stimulating hormone and cortisol in stallions , 1988 .

[16]  Masanori Yamamoto,et al.  Response of the Isolated Human Seminiferous Tubule to Prostaglandins F1ɑ, F2ɑ, E1 and E2 , 1987 .

[17]  D. Thompson,et al.  Patterns of secretion of luteinizing hormone, follicle stimulating hormone and testosterone in stallions during the summer and winter. , 1985, Journal of Animal Science.

[18]  M. Mattioli,et al.  Androstenedione and testosterone concentrations in plasma and milk of the cow throughout pregnancy. , 1984, Journal of reproduction and fertility.

[19]  H. M. Beier,et al.  Effects on fertilization of rabbits of insemination with ejaculates treated with PG-dehydrogenase and antisera to PGE-2 and PGF-2 alpha. , 1983, Journal of reproduction and fertility.

[20]  E. Seren,et al.  Plasma cortisol variations induced in the stallion by mating. , 1983, Acta endocrinologica.

[21]  M. Mattioli,et al.  Concentrations of oestrone sulphate, androgens and LH in the peripheral plasma of mating stallions. , 1982, Journal of reproduction and fertility.

[22]  J. W. Turner,et al.  Influence of prostaglandin F2α on sperm production and seminal characteriticss of the stallion , 1981 .

[23]  J. Diehl,et al.  Serum luteinizing hormone, testosterone prolactin and corticol concentrations after PGF2α in the boar , 1981 .

[24]  E. Nieschlag,et al.  The influence of prostaglandins on sperm motility. , 1981, Prostaglandins.

[25]  D. Thompson,et al.  Sexual behavior, seminal pH and accessory sex gland weights in geldings administered testosterone and(or) estradiol-17 beta. , 1980, Journal of animal science.

[26]  L. Ellis,et al.  In-vitro contractility of rat seminiferous tubules in response to prostaglandins, cyclic GMP, testosterone and 2,4'-dibromoacetophenone. , 1980, Journal of reproduction and fertility.

[27]  J. Cox,et al.  Adrenal-testis interaction in the stallion. , 1979, Equine veterinary journal.

[28]  J. Raeside.  Seasonal changes in the concentration of estrogens and testosterone in the plasma of the stallion , 1979 .

[29]  L. Edqvist,et al.  Plasma Cortisol in the Horse, Diurnal Rhythm and Effects of Exogenous Acth , 1979, Acta Veterinaria Scandinavica.

[30]  F. Kamel,et al.  Hormone release during mating in the male rat: time course, relation to sexual behavior, and interaction with handling procedures. , 1978, Endocrinology.

[31]  W. Wright,et al.  The influence of mating and related stimuli on plasma levels of luteinizing hormone, follicle stimulating hormone, prolactin, and testosterone in the male rat. , 1977, Endocrinology.

[32]  J. Kirkpatrick,et al.  Diurnal variation of plasma corticosteroids in the wild horse stallion , 1977 .

[33]  G. Seidel,et al.  Reproductive physiology of the stallion. VI. Seminal and behavioral characteristics. , 1976, Journal of animal science.

[34]  A. Ågmo.  Serum luteinizing hormone and testosterone after sexual stimulation in male rabbits. , 1976, Acta physiologica Scandinavica.

[35]  R. Liptrap,et al.  Increase in plasma testosterone concentrations after injection of adrenocorticotrophin into the boar. , 1975, The Journal of endocrinology.

[36]  E. Convey,et al.  Blood serum testosterone after sexual preparation or ejaculation, or after injections of LH or prolactin in bulls. , 1973, Journal of animal science.

[37]  E. Convey,et al.  Serum levels of LH, prolactin and growth hormone after ejaculation in bulls. , 1971, Biology of reproduction.

[38]  E. Bedrak,et al.  Steroid biosynthesis by the equine testis. , 1969, Endocrinology.

[39]  J. Raeside.  The isolation of estrone sulfate and estradiol-17 beta sulfate from stallion testes. , 1969, Canadian journal of biochemistry.

[40]  M. Nyman,et al.  Biosynthesis of estrogen by the perfused stallion testis. , 1959, The Journal of biological chemistry.