Phytoecdysteroids of Diploclisia glaucescens seed.

Five phytoecdysteroids were isolated from the seeds of D. glaucescens and identified by spectrometric methods (1H-NMR, 13C-NMR and CIMS [chemical ionization mass spectrometry]). One of them, 24-epi-makisterone A, was not previously reported. The other four, 20-hydroxyecdysone, makisterone A, 24(28)-dehydromakisterone A and pterosterone, were known previously. The 13C-NMR spectrum of 24(28)-dehydromakisterone A is presented for the first time.