Molecular and Serological Detection of Canine Distemper Virus (CDV) in Rural Dogs, Iran

Background and Aims: Canine distemper (CD) is a deadly infectious disease of Canidae family. CD is a multi-systemic viral disease and is specified by wide range of clinical symptoms. The manifestations are not always indicative of CD, therefore a laboratory confirmation is necessary for suspected cases. Materials and Methods: Different clinical specimens of 19 CD suspected unvaccinated dogs were examined for canine distemper virus (CDV) infection by reverse transcription polymerase chain reaction (RT-PCR), Nested-PCR, and serum neutralization (SN) test during 2008-2011. RT-nested PCR assay was adjusted for detection of CDV nucleoprotein (NP) in prepared samples. Results: In samples of 3 out of 19 (15%) dogs, CDV NP gene was confirmed by RT-PCR while RT-PCR and combination with Nested-PCR (RT-nested PCR) presence of CDV NP gene was detected in various samples of 14 (73%) dogs. So efficiency of RT-PCR along with Nested-PCR raised 58%. Among different kinds of obtained samples, conjunctival swabs and kidney tissue biopsies were found to be suitable for analysis of CDV RNA. Additionally CDV antibody was detected in 11 out of 18 serum samples (61%) by SN test, but detection of neutralizing antibodies didn't comply with RT-nested PCR results. Conclusion: Results of this study indicated that Nested-PCR is a sensitive and applicable method for the diagnosis of CDV.

[1]  Wei Wu,et al.  Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china , 2011, Virology Journal.

[2]  M. Rabbani,et al.  Comparative evaluation of clinical samples from naturally infected dogs for early detection of canine distemper virus , 2010, Turkish Journal of Veterinary & Animal Sciences.

[3]  R. Ulloa-Arvizu,et al.  Identification of a genetic variant of canine distemper virus from clinical cases in two vaccinated dogs in Mexico. , 2008, Veterinary journal.

[4]  N. Mattion,et al.  Detection by RT-PCR and genetic characterization of canine distemper virus from vaccinated and non-vaccinated dogs in Argentina. , 2007, Veterinary microbiology.

[5]  R. Avizeh,et al.  Antibody titers against canine distemper virus in unvaccinated rural dogs from Ahvaz, Iran. , 2007, Pakistan journal of biological sciences : PJBS.

[6]  T. Kozma,et al.  Genetic diversity of Hungarian canine distemper virus strains , 2007, Veterinary Microbiology.

[7]  A. Alfieri,et al.  Antemortem Diagnosis of CDV Infection by RT-PCR in Distemper Dogs with Neurological Deficits without the Typical Clinical Presentation , 2006, Veterinary Research Communications.

[8]  S. Pak,et al.  Comparison of tissue and fluid samples for the early detection of canine distemper virus in experimentally infected dogs. , 2006, The Journal of veterinary medical science.

[9]  H. S. Morais,et al.  Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis. , 2006, Research in veterinary science.

[10]  A. Jóźwik,et al.  Comparison of the Immunofluorescence Assay with RT-PCR and Nested PCR in the Diagnosis of Canine Distemper , 2005, Veterinary Research Communications.

[11]  A. Rzeżutka,et al.  Application of N-PCR for diagnosis of distemper in dogs and fur animals. , 2002, Veterinary microbiology.

[12]  H. Yoo,et al.  Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR. , 2001, Journal of veterinary science.

[13]  W. Baumgärtner,et al.  Detection of Canine Distemper Virus Nucleoprotein RNA by Reverse Transcription-PCR Using Serum, Whole Blood, and Cerebrospinal Fluid from Dogs with Distemper , 1999, Journal of Clinical Microbiology.

[14]  W. Baumgärtner,et al.  Dominating interleukin-10 mRNA expression induction in cerebrospinal fluid cells of dogs with natural canine distemper virus induced demyelinating and non-demyelinating CNS lesions , 1999, Journal of Neuroimmunology.

[15]  C. Kai,et al.  Epidemiological observations on recent outbreaks of canine distemper in Tokyo area. , 1996, The Journal of veterinary medical science.

[16]  Carmichael Le,et al.  Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. , 1980 .

[17]  L. Carmichael,et al.  Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. , 1980, American journal of veterinary research.