MaRX: An Approach to Genetics in Mammalian Cells

In this Tech.View, David Beach and his colleagues describe a new, widely applicable genetic method for analysis of gene function in mammalian cells. This MaRX system allows the inhibition of specified genes (effectively producing a null phenotype) and the subsequent analysis of the phenotype.

[1]  G. Hannon,et al.  Loss-of-function genetics in mammalian cells: the p53 tumor suppressor model. , 2000, Nucleic acids research.

[2]  L. Kedes,et al.  Twist is a potential oncogene that inhibits apoptosis. , 1999, Genes & development.

[3]  G. Hannon,et al.  p53-Independent Role of MDM2 in TGF-β1 Resistance , 1998 .

[4]  G. Sczakiel,et al.  In vitro and in vivo action of antisense RNA , 1996, Molecular biotechnology.

[5]  ColdSpring Harbor,et al.  Cold Spring Harbor Laboratory , 2014, Current Biology.

[6]  Robert Kay,et al.  Expression cloning of oncogenes by retroviral transfer of cDNA libraries , 1995, Molecular and cellular biology.

[7]  A. Kimchi,et al.  Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. , 1995, Genes & development.

[8]  I. Roninson,et al.  Isolation of genetic suppressor elements, inducing resistance to topoisomerase II-interactive cytotoxic drugs, from human topoisomerase II cDNA. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[9]  M. Wigler,et al.  Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells , 1982, Nature.

[10]  P. Nurse,et al.  High-frequency transformation of the fission yeast Schizosaccharomyces pombe , 1981, Nature.

[11]  J. Beggs.  Transformation of yeast by a replicating hybrid plasmid , 1978, Nature.

[12]  G. Fink,et al.  Transformation of yeast. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[13]  H. Ozer,et al.  Temperature-sensitive mutants of Balb/3T3 cells: Description of a mutant affected in cellular and polyoma virus DNA synthesis , 1976, Cell.