Development of an immunofluorescence focus assay for Ebola virus

A 48-h indirect immunofluorescence focus assay for the quantitation of Ebola virus was developed, utilizing HeLa-229 cell monolayers. The dose dependency and the sensitivity of this assay as compared with conventional assays are reported. This indirect immunofluorescence focus assay can be used as a rapid, quantitative test for the detection of Ebola virus, an agent from Africa known to cause hemorrhagic fever.

[1]  J. McCormick,et al.  Biologic differences between strains of Ebola virus from Zaire and Sudan. , 1983, The Journal of infectious diseases.

[2]  J. McCormick,et al.  Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation , 1982, Journal of clinical microbiology.

[3]  J B Moe,et al.  Plaque Assay for Ebola Virus , 1981, Journal of clinical microbiology.

[4]  K. Johnson,et al.  Virion nucleic acid of Ebola virus , 1980, Journal of virology.

[5]  K. Johnson,et al.  Ebola virus: identification of virion structural proteins. , 1980, The Journal of general virology.

[6]  A. Truant,et al.  A Latent Infection of Baby Hamster Kidney-21 Cells with Mumps Virus , 1977, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[7]  A. Truant,et al.  A persistent infection of baby hamster kidney‐21 cells with mumps virus and the role of temperature‐sensitive variants , 1977, Journal of medical virology.

[8]  J. V. Lange,et al.  Indirect immunofluorescence for the diagnosis of Lassa fever infection. , 1975, Bulletin of the World Health Organization.

[9]  C. Ray,et al.  Use of immunoperoxidase for the rapid identification of human myxoviruses and paramyxoviruses in tissue culture. , 1974, Applied microbiology.

[10]  J. S. St. Geme,et al.  A Rapid Hemadsorption Plaque Assay for Mumps Virus 1 , 1971, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[11]  T. D. Flanagan,et al.  Plaque formation by mumps virus and inhibition by antiserum. , 1970, Applied microbiology.

[12]  R. Hanson,et al.  Relationship of Plaque Size and Virulence for Chickens of 14 Representative Newcastle Disease Virus Strains , 1968, Journal of virology.

[13]  K. Smith,et al.  Fluorescent Focus Assay of Viruses on Cell Monolayers in Plastic Petri Plates , 1967, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[14]  F. Rapp.  PLAQUE DIFFERENTIATION AND REPLICATION OF VIRULENT AND ATTENUATED STRAINS OF MEASLES VIRUS , 1964, Journal of bacteriology.

[15]  A. Granoff,et al.  Plaque formation with mumps virus. , 1961, Virology.

[16]  R. Deibel,et al.  Location of noncytopathic myxovirus plaques by hemadsorption. , 1960, Virology.