Direct Electrochemistry of Glucose Oxidase at a Gold Electrode Modified with Single-Wall Carbon Nanotubes

The direct electrochemistry of glucose oxidase (GOD) was accomplished at a gold electrode modified with single-wall carbon nanotubes (SWNTs). A pair of welldefined redox peaks was obtained for GOD with the reduction peak potential at –0.465 V and a peak potential separation of 23 mV at pH 7.0. Both FT-IR spectra and the dependence of the reduction peak current on the scan rate revealed that GOD adsorbed onto the SWNT surfaces. The redox wave corresponds to the redox center of the flavin adenine dinucleotide(FAD) of the GOD adsorbate. The electron transfer rate of GOD redox reaction was greatly enhanced at the SWNT-modified electrode. The peak potential was shown to be pH dependent. Verified by spectral methods, the specific enzyme activity of GOD adsorbates at the SWNTs appears to be retained.

[1]  A. Guadalupe,et al.  Direct electrochemistry of horseradish peroxidase adsorbed on glassy carbon electrode from organic solutions , 1997 .

[2]  Richard J. Coles,et al.  Protein electrochemistry at carbon nanotube electrodes , 1997 .

[3]  P. Ajayan.  Nanotubes from Carbon. , 1999, Chemical reviews.

[4]  A. Bond,et al.  Electrochemistry of xanthine oxidase at glassy carbon and mercury electrodes , 1991 .

[5]  A. Rinzler,et al.  Electronic structure of atomically resolved carbon nanotubes , 1998, Nature.

[6]  Malcolm L. H. Green,et al.  Bioelectrochemical single-walled carbon nanotubes. , 2002, Journal of the American Chemical Society.

[7]  W. Heer.  Recent developments in carbon nanotubes , 1999 .

[8]  X. Chen,et al.  Characterization for didodecyldimethylammonium bromide liquid crystal film entrapping catalase with enhanced direct electron transfer rate. , 2001, Biosensors & bioelectronics.

[9]  Yuehe Lin,et al.  Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes , 2002 .

[10]  H. S. Wolff,et al.  iRun: Horizontal and Vertical Shape of a Region-Based Graph Compression , 2022, Sensors.

[11]  Wlodzimierz Kutner,et al.  Electrocatalytic Properties and Sensor Applications of Fullerenes and Carbon Nanotubes , 2003 .

[12]  E. Wang,et al.  Direct electrochemistry and surface characterization of glucose oxidase adsorbed on anodized carbon electrodes , 1994 .

[13]  Zhennan Gu,et al.  Electrocatalytic Oxidation of Norepinephrine at a Glassy Carbon Electrode Modified with Single Wall Carbon Nanotubes , 2002 .

[14]  Neil Rodrigues,et al.  Extreme Oxygen Sensitivity of Electronic Properties of Carbon Nanotubes , 2022 .

[15]  M. Shim,et al.  Functionalization of Carbon Nanotubes for Biocompatibility and Biomolecular Recognition , 2002 .

[16]  Wei‐De Zhang,et al.  Anodic oxidation of hydrazine at carbon nanotube powder microelectrode and its detection. , 2002, Talanta.

[17]  Qionglin Liang,et al.  Carbon nanotube-intercalated graphite electrodes for simultaneous determination of dopamine and serotonin in the presence of ascorbic acid , 2003 .

[18]  N. Chaniotakis,et al.  Novel carbon materials in biosensor systems. , 2003, Biosensors & bioelectronics.

[19]  Jason J. Davis,et al.  The immobilisation of proteins in carbon nanotubes , 1998 .

[20]  Zhennan Gu,et al.  Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes. , 2002, Analytical chemistry.

[21]  Qingming Luo,et al.  Electrocatalytic oxidation of cysteine at carbon nanotube powder microelectrode and its detection , 2003 .

[22]  Charles M. Lieber,et al.  Covalently-Functionalized Single-Walled Carbon Nanotube Probe Tips for Chemical Force Microscopy , 1998 .

[23]  W. D. Heer,et al.  Electrostatic deflections and electromechanical resonances of carbon nanotubes , 1999, Science.

[24]  S. Saito.  Carbon Nanotubes for Next-Generation Electronics Devices , 1997, Science.

[25]  Zhennan Gu,et al.  Investigation of the electrocatalytic behavior of single-wall carbon nanotube films on an Au electrode , 2002 .

[26]  Pulickel M. Ajayan,et al.  Carbon nanotube electrode for oxidation of dopamine , 1996 .

[27]  Yuehe Lin,et al.  Solubilization of carbon nanotubes by Nafion toward the preparation of amperometric biosensors. , 2003, Journal of the American Chemical Society.

[28]  Jun Liu,et al.  Carbon nanotube-modified electrodes for the simultaneous determination of dopamine and ascorbic acid. , 2002, The Analyst.

[29]  S. Dong,et al.  Amperometric glucose biosensor based on lipid film. , 2000, Biosensors & bioelectronics.

[30]  Shengshui Hu,et al.  Simultaneous determination of dopamine and serotonin on a glassy carbon electrode coated with a film of carbon nanotubes. , 2003, Analytical biochemistry.

[31]  H B Broughton,et al.  Molecular modeling. , 2020, Current opinion in chemical biology.

[32]  Guoan Luo,et al.  The Electrocatalytic Oxidation of Thymine at α‐Cyclodextrin Incorporated Carbon Nanotube‐Coated Electrode , 2003 .

[33]  Kong,et al.  Nanotube molecular wires as chemical sensors , 2000, Science.

[34]  C. Lieber,et al.  Atomic structure and electronic properties of single-walled carbon nanotubes , 1998, Nature.

[35]  Richard M. Crooks,et al.  Electrochemistry Using Single Carbon Nanotubes , 1999 .

[36]  Z. Gu,et al.  Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode. , 2001, Analytical chemistry.

[37]  Qingming Luo,et al.  Direct electrochemistry of horseradish peroxidase at carbon nanotube powder microelectrode , 2002 .

[38]  Guoan Luo,et al.  A selective voltammetric method for uric acid detection at beta-cyclodextrin modified electrode incorporating carbon nanotubes. , 2002, The Analyst.

[39]  Ya‐Ping Sun,et al.  Attaching Proteins to Carbon Nanotubes via Diimide-Activated Amidation , 2002 .

[40]  Wei‐De Zhang,et al.  Direct Electron Transfer of Glucose Oxidase Molecules Adsorbed onto Carbon Nanotube Powder Microelectrode , 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[41]  Jing-Juan Xu,et al.  Interfacing cytochrome c to electrodes with a DNA: carbon nanotube composite film , 2002 .

[42]  Huangxian Ju,et al.  Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. , 2003, Biosensors & bioelectronics.