Mutations of the adenylyl cyclase gene that block RAS function in Saccharomyces cerevisiae.

The interaction between RAS proteins and adenylyl cyclase was studied by using dominant interfering mutations of adenylyl cyclase from the yeast Saccharomyces cerevisiae. RAS proteins activate adenylyl cyclase in this organism. A plasmid expressing a catalytically inactive adenylyl cyclase was found to interfere dominantly with this activation. The interfering region mapped to the leucine-rich repeat region of adenylyl cyclase, which is homologous to domains present in several other proteins and is thought to participate in protein-protein interactions.

[1]  P. Seeburg,et al.  Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family. , 1989, Science.

[2]  M. Wigler,et al.  Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[3]  R. Tjian,et al.  Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers. , 1989, Science.

[4]  S. McKnight,et al.  The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite. , 1989, Science.

[5]  T. Curran,et al.  Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains. , 1989, Science.

[6]  J. Hofsteenge,et al.  Amino acid sequence of the ribonuclease inhibitor from porcine liver reveals the presence of leucine-rich repeats. , 1988, Biochemistry.

[7]  S. McKnight,et al.  The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. , 1988, Science.

[8]  K. Fujikawa,et al.  The alpha and beta chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[9]  S. Zipursky,et al.  Chaoptin, a cell surface glycoprotein required for Drosophila photoreceptor cell morphogenesis, contains a repeat motif found in yeast and human , 1988, Cell.

[10]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[11]  K. Anderson,et al.  The Toll gene of drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein , 1988, Cell.

[12]  I. Herskowitz.  Functional inactivation of genes by dominant negative mutations , 1987, Nature.

[13]  K. Titani,et al.  Amino acid sequence of the von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[14]  K. Fujikawa,et al.  Cloning of the alpha chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich alpha 2-glycoprotein. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Wigler,et al.  Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[16]  E. Scolnick,et al.  Mutant ras-encoded proteins with altered nucleotide binding exert dominant biological effects. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[17]  M. Wigler,et al.  DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase , 1985, Cell.

[18]  Y. Takahashi,et al.  Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[19]  M. Wigler,et al.  In yeast, RAS proteins are controlling elements of adenylate cyclase , 1985, Cell.

[20]  M. Wigler,et al.  Genetic analysis of yeast RAS1 and RAS2 genes , 1984, Cell.

[21]  J. Bennetzen,et al.  The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. , 1982, The Journal of biological chemistry.

[22]  G. Fink,et al.  Laboratory course manual for methods in yeast genetics , 1986 .