STUDIES OF LIQUID ADSORPTION CHROMATOGRAPHY PROCESS WITH MIXED MOBILE PHASES

Description of chromatographic retention mechanism in the systems with ternary mobile phase is very complex. Designation of the effect of individual mobile phase components on this process is difficult. The number of moles (or molecules) of less polar solvents of mobile phase referring to one mole (or molecule) of polar solvent must be taken into account. In this connection, a new method of experimental data presentation was proposed. The association effects and changes of surface phase composition were analysed. Experimental data are presented as relationships RM vs. (x1/x2)3 and RM vs. (x1/x3)3. The influence of a chromatographed substance on the retention process was also analysed. The relationships mentioned above enable the effect of individual solvent on the chromatographic process.

[1]  O. Sticher,et al.  PRISMA model for computer-aided HPLC mobile phase optimization based on an automatic peak indentification approach , 1991 .

[2]  B. Ościk-mendyk.  Analysis of Chromatographic Parameters in the Systems with Ternary Mobile Phases. II. Two Polar Solvents in Mobile Phase , 1989 .

[3]  J. A. Jaroniec,et al.  Studies of Association Effects in Liquid Adsorption Chromatography with Ternary Mobile Phases , 1987 .

[4]  B. Ościk-mendyk,et al.  Analysis of Chromatographic Parameters in the Systems with Ternary Mobile Phases , 1987 .

[5]  S. Hara,et al.  Ternary solvent system design for liquid-solid chromatography , 1982 .

[6]  M. Jaroniec,et al.  Simple model of liquid-solid chromatography involving solute-solvent and solvent-solvent interactions , 1981 .

[7]  E. Soczewiński,et al.  Solvent composition effects in the liquid—solid chromatography of azo dyes , 1978 .

[8]  B. Ościk-mendyk.  Mechanism of liquid adsorption chromatography in thin-layer chromatography with ternary mobile phases , 1991 .

[9]  T. Dzido,et al.  Modification of a horizontal sandwich chamber for thin-layer chromatography , 1990 .

[10]  O. Sticher,et al.  Correlation and Prediction of the k′ Values for Mobile Phase Optimization in HPLC , 1989 .

[11]  O. Sticher,et al.  Optimization of the Mobile Phase for HPLC Separation of S-Alk(en)yl-L-Cysteine Derivatives and Their Corresponding Sulfoxide Isomers , 1989 .

[12]  M. Jaroniec,et al.  Theoretical description of association effects in liquid adsorption chromatography with a mixed mobile phase , 1984 .

[13]  M. Jaroniec,et al.  A Model of Liquid Adsorption Chromatography Involving Solute-Solvent Interaction in the Mobile Phase, Energetic Heterogeneity of the Adsorbent, and Differences in Molecular Sizes of Solute and Solvents , 1981 .

[14]  L. Snyder.  Principles of adsorption chromatography , 1968 .