Genetic variations in S gene of porcine epidemic diarrhoea virus from 2018 in Sichuan Province, China

Abstract Porcine epidemic diarrhoea virus (PEDV) belongs to the family Coronavirus, a genus of coronavirus, a highly contact‐infectious intestinal disease pathogen. In this study, we downloaded 62 PEDV S gene sequences uploaded to GenBank, including 10 uploaded by our laboratory from 2018, and constructed a PEDV S gene evolution tree using MEGA V7.0 software. Phylogenetic tree analysis indicated that the genogroup of PEDV in Sichuan Province was divided into three coexisting genogroups (GII‐a, GII‐b and GI‐a), of them, GII‐a has become the main genogroup in the province due to its prevalence and range of spread. Amino acid sequence analysis showed that there were amino acid insertions and deletions in the S protein encoded by the amplified S gene, and there were amino acid mutations in the COE and SS6 of the epitope in the amplified S protein. These results provide a basic research theory for understanding the prevalence of PEDV variation and controlling PED in Sichuan.

[1]  T. Opriessnig,et al.  Evaluation of the efficacy of a commercial inactivated genogroup 2b-based porcine epidemic diarrhea virus (PEDV) vaccine and experimental live genogroup 1b exposure against 2b challenge , 2017, Veterinary Research.

[2]  S. Xiao,et al.  Porcine epidemic diarrhea in China , 2016, Virus Research.

[3]  Changhee Lee,et al.  Efficacy of an inactivated genotype 2b porcine epidemic diarrhea virus vaccine in neonatal piglets , 2016, Veterinary Immunology and Immunopathology.

[4]  Lei Chen,et al.  Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge , 2016, Virology Journal.

[5]  S. Xiao,et al.  Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains , 2016, Viruses.

[6]  Jianfei Chen,et al.  Erratum to: Immunogenicity and antigenic relationships among spike proteins of porcine epidemic diarrhea virus subtypes G1 and G2 , 2016, Archives of Virology.

[7]  Jianfei Chen,et al.  Erratum to: Immunogenicity and antigenic relationships among spike proteins of porcine epidemic diarrhea virus subtypes G1 and G2 , 2016, Archives of Virology.

[8]  Changhee Lee.  Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus , 2015, Virology Journal.

[9]  E. Nelson,et al.  An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration , 2015, BMC Veterinary Research.

[10]  Changhee Lee,et al.  Immunogenicity and protective efficacy of recombinant S1 domain of the porcine epidemic diarrhea virus spike protein , 2014, Archives of Virology.

[11]  Gaiping Zhang,et al.  Phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in central China based on the ORF3 gene and the main neutralization epitopes , 2013, Archives of Virology.

[12]  Li Fang,et al.  Origin, Evolution, and Genotyping of Emergent Porcine Epidemic Diarrhea Virus Strains in the United States , 2013, mBio.

[13]  K. Schwartz,et al.  Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences , 2013, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[14]  Yin Jiang,et al.  Complete Genome Sequence of Porcine Epidemic Diarrhea Virus Strain USA/Colorado/2013 from the United States , 2013, Genome Announcements.

[15]  N. Feng,et al.  Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012 , 2013, Viruses.

[16]  Dong-sheng Gao,et al.  Genetic properties of endemic Chinese porcine epidemic diarrhea virus strains isolated since 2010 , 2013, Archives of Virology.

[17]  Yi-jing Li,et al.  Construction of recombinant lactobacilli expressing the core neutralizing epitope (COE) of porcine epidemic diarrhea virus and a fusion protein consisting of COE and Escherichia coli heat-labile enterotoxin B, and comparison of the immune responses by orogastric immunization. , 2012, Canadian journal of microbiology.

[18]  M. Liao,et al.  Complete Genome Sequence of a Highly Prevalent Isolate of Porcine Epidemic Diarrhea Virus in South China , 2012, Journal of Virology.

[19]  Qigai He,et al.  New Variants of Porcine Epidemic Diarrhea Virus, China, 2011 , 2012, Emerging infectious diseases.

[20]  D. Song,et al.  Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines , 2012, Virus Genes.

[21]  De-kun Chen,et al.  Outbreak of Porcine Epidemic Diarrhea in Suckling Piglets, China , 2012, Emerging infectious diseases.

[22]  N. Wolfe,et al.  Outbreak of Porcine Epidemic Diarrhea in Suckling Piglets, China , 2011 .

[23]  M. Pensaert,et al.  Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy , 2008, Veterinary Record.

[24]  Li Feng,et al.  Spike protein region (aa 636789) of porcine epidemic diarrhea virus is essential for induction of neutralizing antibodies. , 2007, Acta virologica.

[25]  Tae-Geum Kim,et al.  Cloning and sequence analysis of the Korean strain of spike gene of porcine epidemic diarrhea virus and expression of its neutralizing epitope in plants. , 2005, Protein expression and purification.

[26]  M. Pensaert,et al.  A new coronavirus-like particle associated with diarrhea in swine , 2005, Archives of Virology.

[27]  K. Sato,et al.  An immunohistochemical investigation of porcine epidemic diarrhoea , 1995, Journal of Comparative Pathology.

[28]  B. Bloch,et al.  Coronavirus infection in mink (Mustela vision). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus , 1992, Veterinary Microbiology.