Massively parallel pyrosequencing in HIV research.

1411–1415Keywords: DNA sequence, homogeneous PCR products, pyrosequencingThe new massively parallel sequencing methods are soastonishing that one wonders whether space aliens aresecretly behind them. One technician, running a singleinstrument,canobtainuptoapproximately1billionbasesof DNA sequence in a few days. Here we describe thenew sequencing methods, briefly present a few appli-cations in HIV research, and then speculate on futuredirections.

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