Use of CE for the determination of binding constants

In the past two decades, CE has been used frequently for the measurement of apparent binding/association constants. CE has numerous advantages, including short analysis times, low sample consumption, simplicity of operation, etc. This review provides a fundamental introduction to binding theory and then summarizes recent applications and advances in the field of CE‐based methods for the evaluation of molecular association. The time period for this survey is from 2002 (when our previous review was published) to the present.

[1]  P. Righetti,et al.  Computer simulation of affinity capillary electrophoresis , 2002, Electrophoresis.

[2]  W. Ding,et al.  Application of cyclodextrin‐mediated capillary electrophoresis to determine the apparent binding constants and thermodynamic parameters of the alkylnaphthalene derivatives , 2005, Electrophoresis.

[3]  J. Greve,et al.  Abstracts of papers , 2005, Pharmaceutisch Weekblad.

[4]  M. Schwarz,et al.  Electrophoretic affinity measurements on microchip. Determination of binding affinities between diketopiperazine receptors and peptide ligands , 2007, Electrophoresis.

[5]  C. Colyer,et al.  Chromatographic and Electrophoretic Methods for the Determination of Binding Constants for Dye-Protein Complexes , 2008 .

[6]  W. Heineman,et al.  Protein‐aptamer binding studies using microchip affinity capillary electrophoresis , 2008, Electrophoresis.

[7]  S. Jacobson,et al.  Microchip device for performing enzyme assays. , 1997, Analytical chemistry.

[8]  Hailin Wang,et al.  Organic osmolyte mediated kinetic capillary electrophoresis for study of protein-DNA interactions. , 2009, Analytical chemistry.

[9]  S. Weber,et al.  Determination of binding constants by affinity capillary electrophoresis, electrospray ionization mass spectrometry and phase-distribution methods. , 2008, Trends in analytical chemistry : TRAC.

[10]  P. Dubin,et al.  Measurement of the binding of proteins to polyelectrolytes by frontal analysis continuous capillary electrophoresis. , 1997, Analytical chemistry.

[11]  Sergey N Krylov,et al.  Kinetic CE: Foundation for homogeneous kinetic affinity methods , 2007, Electrophoresis.

[12]  L. Blomberg,et al.  Binding of Ca2+, Mg2+, and heparin by human serum amyloid P component in affinity capillary electrophoresis , 2006, Electrophoresis.

[13]  N. Heegaard,et al.  A novel specific heparin‐binding activity of bovine folate‐binding protein characterized by capillary electrophoresis , 2006, Electrophoresis.

[14]  S. Jacobson,et al.  Computer simulations of electrokinetic injection techniques in microfluidic devices , 2000, Analytical chemistry.

[15]  F. Gomez,et al.  Determination of Binding Constants of Polyethylene Glycol Vancomycin Derivatives to Peptide Ligands Using Affinity Capillary Electrophoresis , 2007 .

[16]  M. Schwarz,et al.  Microchip Affinity Capillary Electrophoresis: Applications and Recent Advances , 2006 .

[17]  F. Gomez,et al.  Use of a Dual-Marker Form of Analysis to Estimate Binding Constants Between Receptors and Ligands by Affinity Capillary Electrophoresis , 2004 .

[18]  Sergey N Krylov,et al.  Non-equilibrium capillary electrophoresis of equilibrium mixtures--appreciation of kinetics in capillary electrophoresis. , 2003, The Analyst.

[19]  Ying Sun,et al.  Capillary electrophoresis frontal analysis for characterization of alphavbeta3 integrin binding interactions. , 2008, Analytical chemistry.

[20]  M. Berezovski,et al.  Kinetic capillary electrophoresis (KCE): a conceptual platform for kinetic homogeneous affinity methods. , 2005, Journal of the American Chemical Society.

[21]  F. Gomez,et al.  Frontal analysis microchip capillary electrophoresis to study the binding of ligands to receptors derivatized on magnetic beads , 2009, Analytical and bioanalytical chemistry.

[22]  Sergey N. Krylov,et al.  Kinetic capillary electrophoresis-based affinity screening of aptamer clones. , 2009, Analytica chimica acta.

[23]  Claudia B. Cohen,et al.  A microchip-based enzyme assay for protein kinase A. , 1999, Analytical biochemistry.

[24]  Victor Okhonin,et al.  Sweeping capillary electrophoresis: a non-stopped-flow method for measuring bimolecular rate constant of complex formation between protein and DNA. , 2004, Journal of the American Chemical Society.

[25]  T. Cabras,et al.  Different binding thermodynamics of Ni2+, Cu2+, and Zn2+ to bacitracin A1 determined by capillary electrophoresis , 2004, Electrophoresis.

[26]  A. Stalcup,et al.  Affinity capillary electrophoresis and isothermal titration calorimetry for the determination of fatty acid binding with beta-cyclodextrin. , 2008, Journal of chromatography. A.

[27]  M. Schwarz,et al.  Quantification of single‐stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis – Part II , 2008, Electrophoresis.

[28]  D. Winzor.  A need for caution in the use of frontal analysis continuous capillary electrophoresis for the determination of ligand binding data. , 2006, Analytical biochemistry.

[29]  J. Vacík,et al.  Computer-aided simulation of electromigration , 1991 .

[30]  I. Krull,et al.  Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein. , 2004, Journal of chromatography. A.

[31]  Michael A. Rodriguez,et al.  Estimation of association constants between oral malodor components and various native and derivatized cyclodextrins , 2006 .

[32]  S. Ermakov,et al.  Computer simulation of transient states in capillary zone electrophoresis and isotachophoresis , 1992, Electrophoresis.

[33]  W. Ding,et al.  Application of cyclodextrin-mediated capillary electrophoresis to simultaneously determine the apparent binding constants and thermodynamic parameters of naphthalenesulfonate derivatives. , 2006, Talanta.

[34]  D. Saville,et al.  Computer simulation and experimental validation of the electrophoretic behavior of proteins. , 1989, Analytical chemistry.

[35]  P. Dubin,et al.  Characterization of polyanion-protein complexes by frontal analysis continuous capillary electrophoresis and small angle neutron scattering: effect of polyanion flexibility. , 2005, Analytical biochemistry.

[36]  G. Cheng,et al.  The formation of cucurbit[n]uril (n = 6, 7) complexes with amino compounds in aqueous formic acid studied by capillary electrophoresis , 2005, Electrophoresis.

[37]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[38]  David D Y Chen,et al.  Enumeration algorithm for determination of binding constants in capillary electrophoresis. , 2005, Analytical chemistry.

[39]  Studies of binding constants and interaction of drugs to trans-activation response RNA by capillary electrophoresis , 2005 .

[40]  T. Le Saux,et al.  Measurement of monomolecular binding constants of neutral phenols into the beta-cyclodextrin by continuous frontal analysis in capillary and microchip electrophoresis via a competitive assay. , 2006, Journal of chromatography. A.

[41]  Joel C. Colburn,et al.  Capillary electrophoresis : theory & practice , 1992 .

[42]  L. Holland,et al.  Determination of dissociation constants for a heparin‐binding domain of amyloid precursor protein and heparins or heparan sulfate by affinity capillary electrophoresis , 2004, Electrophoresis.

[43]  Grady Hanrahan,et al.  Use of chemometric methodology in optimizing conditions for competitive binding partial filling affinity capillary electrophoresis , 2008, Electrophoresis.

[44]  F. Gomez,et al.  Use of a partial-filling technique in affinity capillary electrophoresis for determining binding constants of ligands to receptors. , 1999, Journal of chromatography. A.

[45]  L. Zhang,et al.  Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis–frontal analysis and multivariate regression , 2009, Analytical and bioanalytical chemistry.

[46]  Huan‐Tsung Chang,et al.  Immunoaffinity capillary electrophoresis: Determination of binding constant and stoichiometry for antibody‐antigen interaction , 2002, Electrophoresis.

[47]  F. Gomez,et al.  Estimation of binding constants between ristocetin and teicoplanin and peptides using on-column ligand derivatization coupled to affinity capillary electrophoresis , 2004, Analytical and bioanalytical chemistry.

[48]  H. Tajmir-Riahi,et al.  AZT binds RNA at multiple sites. , 2004, DNA and cell biology.

[49]  M. Zhong,et al.  Determination of protein-drug binding constants by pressure-assisted capillary electrophoresis (PACE)/frontal analysis (FA). , 2002, Journal of pharmaceutical and biomedical analysis.

[50]  Y. Guillaume,et al.  Salt dependence on vancomycin‐herbicide enantiomer binding: Capillary electrophoresis study and theoretical approach , 2003, Electrophoresis.

[51]  T. Hirokawa,et al.  Numerical simulation for capillary electrophoresis: I. Development of a simulation program with high numerical stability , 1998 .

[52]  V. McGuffin,et al.  Three-dimensional molecular simulation of electrophoretic separations , 1998 .

[53]  Yuanhong Wang,et al.  Affinity CE Determination of the Binding Constant of Bioactive Sulfated Polysaccharide 916 to Human Serum Albumin , 2009 .

[54]  A. Varenne,et al.  Determination of the binding parameters for antithrombin-heparin fragment systems by affinity and frontal analysis continuous capillary electrophoresis. , 2006, Journal of chromatography. A.

[55]  S. Krylov.  Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures (NECEEM): A Novel Method for Biomolecular Screening , 2006, Journal of biomolecular screening.

[56]  G. Guiochon,et al.  High-resolution modeling of capillary zone electrophoresis and isotachophoresis , 1991 .

[57]  B. Lin,et al.  Recent advances in the study of biomolecular interactions by capillary electrophoresis , 2004, Electrophoresis.

[58]  M. Berezovski,et al.  Using nonequilibrium capillary electrophoresis of equilibrium mixtures for the determination of temperature in capillary electrophoresis. , 2004, Analytical chemistry.

[59]  R. Desharnais,et al.  Optimization of conditions for flow-through partial-filling affinity capillary electrophoresis to estimate binding constants of ligands to receptors , 2005 .

[60]  H. Ritter,et al.  Color change of N-isopropylacrylamide copolymer bearing Reichardts dye as optical sensor for lower critical solution temperature and for host-guest interaction with beta-cyclodextrin. , 2007, Journal of the American Chemical Society.

[61]  M. Kokkoris,et al.  THE BOOK OF ABSTRACTS , 2015 .

[62]  M. Berezovski,et al.  Plug-plug kinetic capillary electrophoresis: method for direct determination of rate constants of complex formation and dissociation. , 2006, Analytical chemistry.

[63]  D. J. Harrison,et al.  Monoclonal antibody binding affinity determined by microchip‐based capillary electrophoresis , 1998, Electrophoresis.

[64]  D. F. Waugh,et al.  Protein-protein interactions. , 1954, Advances in protein chemistry.

[65]  C. Meyer,et al.  Estimation of binding constants for the substrate and activator of Rhodobacter sphaeroides adenosine 5'-diphosphate-glucose pyrophosphorylase using affinity capillary electrophoresis. , 2004, Analytical biochemistry.

[66]  Screening of phage displayed human liver cDNA library against dexamethasone. , 2007, Journal of pharmaceutical and biomedical analysis.

[67]  I. Krull,et al.  Application of affinity capillary electrophoresis for the determination of binding and thermodynamic constants of enediynes with bovine serum albumin , 2002, Electrophoresis.

[68]  A. Timerbaev,et al.  Determination of binding constants and stoichiometries for platinum anticancer drugs and serum transport proteins by capillary electrophoresis using the Hummel-Dreyer method. , 2005, Journal of separation science.

[69]  G. Houen,et al.  Affinity capillary electrophoresis for identification and investigation of human Gc‐globulin (vitamin D‐binding protein) and its isoforms interacting with G‐actin , 2008, Electrophoresis.

[70]  F. Gomez,et al.  On-column synthesis coupled to affinity capillary electrophoresis for the determination of binding constants of peptides to glycopeptide antibiotics. , 2004, Journal of chromatography. A.

[71]  M. Bjerrum,et al.  Ca(2+) and Na(+) binding to high affinity sites of calcium-containing proteins measured by capillary electrophoresis. , 2003, Journal of inorganic biochemistry.

[72]  A. Timerbaev,et al.  Comparative binding of antitumor indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] to serum transport proteins assayed by capillary zone electrophoresis. , 2005, Analytical biochemistry.

[73]  Xinxiang Zhang,et al.  Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis , 2007, Analytical and bioanalytical chemistry.

[74]  Laurent Mouchard,et al.  Analysis of virtual two‐dimensional gels based upon affinity capillary electrophoresis hyphenated to ion trap‐mass spectrometry , 2005, Electrophoresis.

[75]  J. Ladbury,et al.  Partial filling multiple injection affinity capillary electrophoresis (PFMIACE) to estimate binding constants of receptors to ligands. , 2007, Talanta.

[76]  M. Taverna,et al.  Determination of binding constants of hydrophobically end-capped poly(ethylene glycol)s with beta-cyclodextrin by affinity capillary electrophoresis. , 2004, Journal of chromatography. A.

[77]  Zhining Xia,et al.  Establishment and application of a new method for the determination of kinetic parameters by plug-plug kinetic capillary electrophoresis (ppKCE) , 2008 .

[78]  T. Ramstad,et al.  Determination of the binding constant between alprostadil and alpha-cyclodextrin by capillary electrophoresis: implications for a freeze-dried formulation. , 2005, Journal of pharmaceutical sciences.

[79]  You-nian Liu,et al.  Estimation of Binding Constants for Diclofenac Sodium and Bovine Serum Albumin by Affinity Capillary Electrophoresis and Fluorescence Spectroscopy , 2008 .

[80]  S. Krylov,et al.  Nonequilibrium capillary electrophoresis of equilibrium mixtures, mathematical model. , 2004, Analytical chemistry.

[81]  F. Gomez,et al.  DETERMINATION OF BINDING CONSTANTS BETWEEN TEICOPLANIN AND D-ALA-D-ALA TERMINUS PEPTIDES BY AFFINITY CAPILLARY ELECTROPHORESIS , 2002 .

[82]  J. Cann,et al.  Theory of isomerization equilibrium in electrophoresis. II. , 1961, Archives of biochemistry and biophysics.

[83]  F. Gomez,et al.  Use of mobility ratios to estimate binding constants of ligands to proteins in affinity capillary electrophoresis. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[84]  S. Terabe,et al.  Estimation of binding constants by capillary electrophoresis. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[85]  E. Pitts.  An extension of the theory of the conductivity and viscosity of electrolyte solutions , 1953, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[86]  F. Gomez,et al.  Determination of binding constants between the antibiotic ristocetin A and D-Ala-D-Ala terminus peptides by affinity capillary electrophoresis , 2003 .

[87]  M. Schwarz,et al.  Affinity capillary electrophoresis on microchips. , 2005, Journal of chromatography. A.

[88]  D. Armstrong,et al.  Partitioning behavior of solutes eluted with micellar mobile phases in liquid chromatography , 1981 .

[89]  F. Gomez,et al.  Multiple-injection affinity capillary electrophoresis to estimate binding constants of receptors to ligands , 2005, Analytical and bioanalytical chemistry.

[90]  H. Boelens,et al.  Comparison of five methods for the study of drug-protein binding in affinity capillary electrophoresis. , 1997, Journal of chromatography. A.

[91]  J. Sonke,et al.  Determination of neodymium-fulvic acid binding constants by capillary electrophoresis inductively coupled plasma mass spectrometry (CE-ICP-MS) , 2004 .

[92]  K. A. Connors,et al.  Binding Constants: The Measurement of Molecular Complex Stability , 1987 .

[93]  M. Bowser,et al.  Monte Carlo Simulation of Error Propagation in the Determination of Binding Constants from Rectangular Hyperbolae. 1. Ligand Concentration Range and Binding Constant , 1998 .

[94]  E. Fattal,et al.  Determination of binding constants of vasoactive intestinal peptide to poly(amidoamine) dendrimers designed for drug delivery using ACE , 2007, Electrophoresis.

[95]  G. Jiang,et al.  Study of protein binding and micellar partition of highly hydrophobic molecules in a single system using capillary electrophoresis , 2008, Electrophoresis.

[96]  V. Kašička,et al.  Determination of dissociation constant of phosphinate group in phosphinic pseudopeptides by capillary zone electrophoresis. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[97]  S. Neya,et al.  NMR Chemical Shift References for Binding Constant Determination in Aqueous Solutions , 2001 .

[98]  Ying Sun,et al.  Computer simulation of different modes of ACE based on the dynamic complexation model , 2007, Electrophoresis.

[99]  A. A. Ouameur,et al.  A Comparative study of Fe(II) and Fe(III) interactions with DNA duplex: major and minor grooves bindings. , 2005, DNA and cell biology.

[100]  D. Armstrong,et al.  Development of an ESI-MS screening method for evaluating binding affinity between integrin fragments and RGD-based peptides , 2007 .

[101]  J. Dybal,et al.  Application of capillary affinity electrophoresis and density functional theory to the investigation of valinomycin-lithium complex. , 2009, Journal of chromatography. A.

[102]  F. Gomez,et al.  Flow-through partial-filling affinity capillary electrophoresis for the estimation of binding constants of ligands to receptors , 1999 .

[103]  G. Caldwell,et al.  Using capillary electrophoresis/frontal analysis to screen drugs interacting with human serum proteins , 1998, Electrophoresis.

[104]  J. Michael Ramsey,et al.  Microfluidic Assays of Acetylcholinesterase Inhibitors , 1999 .

[105]  N. Heegaard,et al.  Capillary electrophoresis frontal analysis: Principles and applications for the study of drug‐plasma protein binding , 2003, Electrophoresis.

[106]  Xin Liu,et al.  Using capillary electrophoresis with laser-induced fluorescence to study the interaction of green fluorescent protein-labeled calmodulin with Ca2+- and calmodulin-binding protein. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[107]  Guonan Chen,et al.  Determination of the binding constant for the inclusion complex between procaine hydrochloride and beta-cyclodextrin by capillary electrophoresis. , 2003, Talanta.

[108]  Frontal analysis on a microchip. , 2002, The Analyst.

[109]  D. Armstrong,et al.  Examination of the origin, variation, and proper use of expressions for the estimation of association constants by capillary electrophoresis , 1996 .

[110]  D. Armstrong,et al.  Methods for the determination of binding constants by capillary electrophoresis , 2001, Electrophoresis.

[111]  F. Gomez,et al.  Through-a-chip partial filling affinity capillary electrophoresis for estimating binding constants of ligands to receptors. , 2008, Talanta.

[112]  D. Armstrong,et al.  On‐line preconcentration of weak electrolytes by electrokinetic accumulation in CE: Experiment and simulation , 2007, Electrophoresis.

[113]  F. Gomez,et al.  Multiple-injection affinity capillary electrophoresis to examine binding constants between glycopeptide antibiotics and peptides. , 2006, Journal of chromatography. A.

[114]  Sergey N Krylov,et al.  Nonequilibrium capillary electrophoresis of equilibrium mixtures--a single experiment reveals equilibrium and kinetic parameters of protein-DNA interactions. , 2002, Journal of the American Chemical Society.

[115]  J. Cann,et al.  Electrophoretic demonstration of specific enzyme-substrate complex between pepsin and serum albumin. , 1961, The Journal of biological chemistry.

[116]  D. J. Harrison,et al.  Microchip-based capillary electrophoresis for immunoassays: analysis of monoclonal antibodies and theophylline. , 1997, Analytical chemistry.

[117]  Michael Musheev,et al.  Nonequilibrium capillary electrophoresis of equilibrium mixtures: a universal tool for development of aptamers. , 2005, Journal of the American Chemical Society.

[118]  D. Winzor.  Determination of binding constants by analogous procedures in size exclusion chromatography and capillary electrophoresis. , 2008, Analytical biochemistry.

[119]  G. Kelbg,et al.  Zur Theorie der Leitfähigkeit starker nicht assoziierender Elektrolyte bei höheren Konzentrationen , 1952 .

[120]  F. Gomez,et al.  Partial-filling affinity capillary electrophoresis , 2003, Analytical and bioanalytical chemistry.

[121]  M. Taverna,et al.  A study of the binding between polymers and peptides, using affinity capillary electrophoresis, applied to polymeric drug delivery systems , 2002, Electrophoresis.

[122]  Grady Hanrahan,et al.  Implementation of chemometric methodology in ACE: Predictive investigation of protein–ligand binding , 2007, Electrophoresis.

[123]  F. Gomez,et al.  On-column ligand synthesis coupled to partial-filling affinity capillary electrophoresis to estimate binding constants of ligands to a receptor. , 2001, Journal of chromatography. A.

[124]  Determination of Complexation Selectivity of Aromatic Phosphonic Acids by Ammonium and Potassium Cations Using CE , 2005 .

[125]  P. Sandra,et al.  Measurement of the binding of retinoic acid to β‐lactoglobulin B by affinity capillary electrophoresis , 2002 .

[126]  J. Østergaard,et al.  Bioanalytical interaction studies executed by preincubation affinity capillary electrophoresis , 2006, Electrophoresis.

[127]  Li Xiaomei,et al.  Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis. , 2007, Journal of chromatography. A.