A novel method for construction of gene fragment library to searching epitopes.

Identification of the epitope sequence or the functional domain of proteins is a laborious process but a necessary one for biochemical and immunological research. To achieve intensive and effective screening of these functional peptides in various molecules, we established a novel screening method using a phage library system that displays various lengths and parts of peptides derived from target protein. Applying this library for epitope mapping, epitope peptide was more efficiently identified from gene fragment library than conventional random peptide library. Our system may be a most powerful method for identifying functional peptides.

[1]  J. Devlin,et al.  Random peptide libraries: a source of specific protein binding molecules. , 1990, Science.

[2]  R. Fischer,et al.  Rapid identification of a tobacco mosaic virus epitope by using a coat protein gene-fragment-pVIII fusion library. , 2001, The Journal of general virology.

[3]  D. Stanworth,et al.  Allergy treatment with a peptide vaccine , 1990, The Lancet.

[4]  T. Kyo,et al.  Induction of WT1 (Wilms' tumor gene)-specific cytotoxic T lymphocytes by WT1 peptide vaccine and the resultant cancer regression. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[5]  I. Haro,et al.  Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design , 2003, FEBS letters.

[6]  Lars Holmgren,et al.  Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastases by a lewis lung carcinoma , 1994, Cell.

[7]  J. McMurray,et al.  Tight‐binding inhibitory sequences against pp60 c−src identified using a random 15‐amino‐acid peptide library , 1996, FEBS letters.

[8]  K. Itoh,et al.  Identification of Peptide Vaccine Candidates for Prostate Cancer Patients with HLA-A3 Supertype Alleles , 2005, Clinical Cancer Research.

[9]  S. Schwarze,et al.  In vivo protein transduction: delivery of a biologically active protein into the mouse. , 1999, Science.

[10]  F. Fack,et al.  Epitope mapping by phage display: random versus gene-fragment libraries. , 1997, Journal of immunological methods.

[11]  Y. Yoshioka,et al.  Optimal construction of non-immune scFv phage display libraries from mouse bone marrow and spleen established to select specific scFvs efficiently binding to antigen. , 2004, Biochemical and biophysical research communications.

[12]  Jamie K. Scott,et al.  Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics , 2001, Current Opinion in Chemical Biology.

[13]  Mikito Yasuzawa,et al.  Selection of novel structural zinc sites from a random peptide library , 2003, FEBS letters.

[14]  E Ruoslahti,et al.  New perspectives in cell adhesion: RGD and integrins. , 1987, Science.

[15]  Y. Okahata,et al.  Selection of ganglioside GM1‐binding peptides by using a phage library , 1999, FEBS letters.

[16]  M. Yamada,et al.  Mutational analysis of structure--activity relationships in human tumor necrosis factor-alpha. , 1990, Protein engineering.

[17]  J. White,et al.  Use of a gene-targeted phage display random epitope library to map an antigenic determinant on the bluetongue virus outer capsid protein VP5. , 1995, Journal of immunological methods.

[18]  A. Plückthun,et al.  Ribosome display: an in vitro method for selection and evolution of antibodies from libraries. , 1999, Journal of immunological methods.

[19]  D. D. du Plessis,et al.  Identification of antigenic regions on VP2 of African horsesickness virus serotype 3 by using phage-displayed epitope libraries. , 2000, The Journal of general virology.

[20]  William Arbuthnot Sir Lane,et al.  Endostatin: An Endogenous Inhibitor of Angiogenesis and Tumor Growth , 1997, Cell.