Plasmonic nanobiosensor based on hairpin DNA for detection of trace oligonucleotides biomarker in cancers.

MicroRNAs (miRNAs), a class of small, endogenous, noncoding RNA molecules, can serve as biomarkers for potential applications in cancer diagnosis, prognosis, and prediction due to its abnormal expression. As a result, a novel label-free biosensor with nanometer scale was prepared and employed in the detection of trace oligonucleotides based on the localized surface plasmon resonance (LSPR). The dielectric constant on the surface of DNA modified gold nanoparticle would change when probe single-strand DNA hybridized with target oligonucleotides, which resulted in the notable red shift of scattering peak position. The biosensor with excellent selectivity can be used in a real-time monitoring hybridization process. Notably, this method provided label-free detection of DNA and miRNA at single nanoparticle level with limit of detection up to 3 nM. Due to the advantages of LSPR scattering spectra, single nanoparticle biosensor can be designed for trace cancer-relevant miRNAs detection in the future.

[1]  N. Huang,et al.  Colorimetric detection of DNA hybridization based on a dual platform of gold nanoparticles and graphene oxide. , 2014, Biosensors & bioelectronics.

[2]  C. Fan,et al.  A methylation-blocked cascade amplification strategy for label-free colorimetric detection of DNA methyltransferase activity. , 2014, Biosensors & bioelectronics.

[3]  S. Sim,et al.  Resonant Rayleigh light scattering of single Au nanoparticles with different sizes and shapes. , 2014, Nanoscale.

[4]  Xiaodong Xia,et al.  Hairpin DNA probe with 5'-TCC/CCC-3' overhangs for the creation of silver nanoclusters and miRNA assay. , 2014, Biosensors & bioelectronics.

[5]  C. Huang,et al.  Real-time dark-field scattering microscopic monitoring of the in situ growth of single Ag@Hg nanoalloys. , 2013, ACS nano.

[6]  Myriam Gorospe,et al.  Correction: Corrigendum: The tRNA methyltransferase NSun2 stabilizes p16INK4 mRNA by methylating the 3′-untranslated region of p16 , 2013, Nature Communications.

[7]  Ying Wang,et al.  Ultrasensitive and selective gold film-based detection of mercury (II) in tap water using a laser scanning confocal imaging-surface plasmon resonance system in real time. , 2013, Biosensors & bioelectronics.

[8]  Eric C. Lai,et al.  Adult-specific functions of animal microRNAs , 2013, Nature Reviews Genetics.

[9]  Yi-Tao Long,et al.  Plasmon resonance scattering spectroscopy at the single-nanoparticle level: real-time monitoring of a click reaction. , 2013, Angewandte Chemie.

[10]  Wei Wei,et al.  DNA-responsive disassembly of AuNP aggregates: influence of nonbase-paired regions and colorimetric DNA detection by exonuclease III aided amplification. , 2013, Journal of materials chemistry. B.

[11]  E. Yeung,et al.  Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles , 2013, Nature Communications.

[12]  Tugba Kilic,et al.  Electrochemical based detection of microRNA, mir21 in breast cancer cells. , 2012, Biosensors & bioelectronics.

[13]  Lauren A Austin,et al.  Real-time molecular imaging throughout the entire cell cycle by targeted plasmonic-enhanced Rayleigh/Raman spectroscopy. , 2012, Nano letters.

[14]  H. Kjaergaard,et al.  Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection. , 2012, ACS nano.

[15]  Kenneth T. V. Grattan,et al.  Optimization of gold-nanoparticle-based optical fibre surface plasmon resonance (SPR)-based sensors , 2012 .

[16]  Andreas Henkel,et al.  Single unlabeled protein detection on individual plasmonic nanoparticles. , 2012, Nano letters.

[17]  Yang Li,et al.  Resonance scattering particles as biological nanosensors in vitro and in vivo. , 2012, Chemical Society reviews.

[18]  Lauren A Austin,et al.  Plasmonic imaging of human oral cancer cell communities during programmed cell death by nuclear-targeting silver nanoparticles. , 2011, Journal of the American Chemical Society.

[19]  Tom Vosch,et al.  Rapid detection of microRNA by a silver nanocluster DNA probe. , 2011, Analytical chemistry.

[20]  I. Willner,et al.  Single gold nanoparticles as real-time optical probes for the detection of NADH-dependent intracellular metabolic enzymatic pathways. , 2011, Angewandte Chemie.

[21]  Itamar Willner,et al.  Chemiluminescent and chemiluminescence resonance energy transfer (CRET) detection of DNA, metal ions, and aptamer-substrate complexes using hemin/G-quadruplexes and CdSe/ZnS quantum dots. , 2011, Journal of the American Chemical Society.

[22]  J. Hafner,et al.  Localized surface plasmon resonance sensors. , 2011, Chemical reviews.

[23]  Harald Giessen,et al.  Nanoantenna-enhanced gas sensing in a single tailored nanofocus , 2011, CLEO: 2011 - Laser Science to Photonic Applications.

[24]  Y. Shu,et al.  MiR-21 overexpression in human primary squamous cell lung carcinoma is associated with poor patient prognosis , 2011, Journal of Cancer Research and Clinical Oncology.

[25]  Yan Du,et al.  Au NPs-enhanced surface plasmon resonance for sensitive detection of mercury(II) ions. , 2010, Biosensors & bioelectronics.

[26]  Taewook Kang,et al.  Picomolar selective detection of mercuric ion (Hg2 + ) using a functionalized single plasmonic gold nanoparticle , 2010, Nanotechnology.

[27]  B. Reinhard,et al.  Calibration of Silver Plasmon Rulers in the 1-25 nm Separation Range: Experimental Indications of Distinct Plasmon Coupling Regimes. , 2010, The journal of physical chemistry. C, Nanomaterials and interfaces.

[28]  H. Masuhara,et al.  Spectral and 3-Dimensional Tracking of Single Gold Nanoparticles in Living Cells Studied by Rayleigh Light Scattering Microscopy† , 2009 .

[29]  H. Lang,et al.  Quantitative time-resolved measurement of membrane protein-ligand interactions using microcantilever array sensors. , 2009, Nature nanotechnology.

[30]  B. Reinhard,et al.  Resolving sub-diffraction limit encounters in nanoparticle tracking using live cell plasmon coupling microscopy. , 2008, Nano letters.

[31]  Wendy Frankel,et al.  MicroRNA-21 is Overexpressed in Pancreatic Cancer and a Potential Predictor of Survival , 2008, Journal of Gastrointestinal Surgery.

[32]  H. Allgayer,et al.  MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer , 2008, Oncogene.

[33]  W. Smith,et al.  Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates. , 2008, Analytical chemistry.

[34]  K. Ghoshal,et al.  MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. , 2007, Gastroenterology.

[35]  D. Fernig,et al.  Determination of size and concentration of gold nanoparticles from UV-vis spectra. , 2007, Analytical chemistry.

[36]  A Paul Alivisatos,et al.  A nanoplasmonic molecular ruler for measuring nuclease activity and DNA footprinting , 2006, Nature nanotechnology.

[37]  Ping Gong,et al.  Surface coverage and structure of mixed DNA/alkylthiol monolayers on gold: characterization by XPS, NEXAFS, and fluorescence intensity measurements. , 2006, Analytical chemistry.

[38]  C. Croce,et al.  MicroRNA gene expression deregulation in human breast cancer. , 2005, Cancer research.

[39]  K. Kosik,et al.  MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. , 2005, Cancer research.

[40]  Arianna Bottoni,et al.  miR‐15a and miR‐16‐1 down‐regulation in pituitary adenomas , 2005, Journal of cellular physiology.

[41]  Carsten Sönnichsen,et al.  A molecular ruler based on plasmon coupling of single gold and silver nanoparticles , 2005, Nature Biotechnology.

[42]  Xiaohua Huang,et al.  Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer. , 2005, Nano letters.

[43]  Itamar Willner,et al.  Amplified detection of telomerase activity using electrochemical and quartz crystal microbalance measurements. , 2004, Biosensors & bioelectronics.

[44]  V. Ambros.  The functions of animal microRNAs , 2004, Nature.

[45]  Y. Yatabe,et al.  Reduced Expression of the let-7 MicroRNAs in Human Lung Cancers in Association with Shortened Postoperative Survival , 2004, Cancer Research.

[46]  M. Natan,et al.  Seeding of Colloidal Au Nanoparticle Solutions. 2. Improved Control of Particle Size and Shape , 2000 .

[47]  Han,et al.  Dodecanethiol-Derivatized Au/Ag Bimetallic Nanoparticles: TEM, UV/VIS, XPS, and FTIR Analysis. , 1998, Journal of colloid and interface science.

[48]  A. Steel,et al.  Electrochemical quantitation of DNA immobilized on gold. , 1998, Analytical chemistry.

[49]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[50]  Tsao-Jen Lin,et al.  Detection of cadmium by a fiber-optic biosensor based on localized surface plasmon resonance. , 2009, Biosensors & bioelectronics.

[51]  Anton J. Enright,et al.  Materials and Methods Figs. S1 to S4 Tables S1 to S5 References and Notes Micrornas Regulate Brain Morphogenesis in Zebrafish , 2022 .