Myeloperoxidase gene expression in blast cells with a lymphoid phenotype in cases of acute lymphoblastic leukemia.

By using a cDNA clone of the myeloperoxidase (MPO) gene, we have studied, by Northern blot analysis, the level of MPO mRNA in eight cases of acute lymphoblastic leukemia (ALL). The blast cell populations studied were characterized by morphologic, cytochemical, immunochemical, and molecular criteria. With all the methods used the populations were found to be highly homogeneous and showed a typical lymphoid phenotype. In particular, the Ig heavy-chain gene rearrangement was largely prevalent, and the germ line configuration was almost absent. However, in three of eight cases, high levels of MPO mRNA were detected. The remarkable homogeneity of the cell populations examined suggests that the MPO mRNA observed was present in cellular elements certainly identified as lymphoid. The absence of contamination by myeloid cells was confirmed by the results of Western blot analysis of the proteins of the cell population studied: no MPO protein was detectable. The levels of mRNA observed were high enough to be comparable to those observed in a promyelocytic cell population.

[1]  S. Nagata,et al.  Chromosomal gene structure of human myeloperoxidase and regulation of its expression by granulocyte colony-stimulating factor. , 1987, The Journal of biological chemistry.

[2]  G. Torelli,et al.  Molecular study of the Philadelphia translocation in chronic myelogenous leukemia in different stages of disease. , 1987, Haematologica.

[3]  A. Carè,et al.  Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species. , 1987, Nucleic acids research.

[4]  E. McCulloch.  Lineage infidelity or lineage promiscuity? , 1987, Leukemia.

[5]  R. Cook,et al.  Human myeloperoxidase gene: molecular cloning and expression in leukemic cells. , 1986, Blood.

[6]  L. Kaczmarek,et al.  Coding sequence and growth regulation of the human vimentin gene , 1986, Molecular and cellular biology.

[7]  C. Pui,et al.  ACUTE LEUKAEMIA WITH MIXED LYMPHOID AND MYELOID PHENOTYPE , 1984, British journal of haematology.

[8]  C. Pui,et al.  Acute leukaemia with mixed lymphoid and myeloid phenotype , 1984, British journal of haematology.

[9]  M. Greaves,et al.  A monoclonal antibody (WT1) for detecting leukemias of T-cell precursors (T-ALL). , 1983, Blood.

[10]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[11]  L. Stanton,et al.  Nucleotide sequence of cloned cDNA of human c-myc oncogene , 1983, Nature.

[12]  L. Smith,et al.  Lineage infidelity in acute leukemia. , 1983, Blood.

[13]  W. Nauseef,et al.  Biochemical and immunologic analysis of hereditary myeloperoxidase deficiency. , 1983, The Journal of clinical investigation.

[14]  H. Blau,et al.  Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed , 1983, Molecular and cellular biology.

[15]  G. Stein,et al.  Organization of human histone genes. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[16]  S. Ferrari,et al.  Sequence complexity and diversity of polyadenylated RNA molecules of human normal resting, PHA-stimulated and leukemic lymphocytes. , 1981, Nucleic acids research.

[17]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[18]  C. Morris,et al.  Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets. , 1980, Analytical biochemistry.

[19]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[20]  M. Greaves,et al.  Lineage promiscuity in hemopoietic differentiation and leukemia. , 1986, Blood.

[21]  S. Ferrari,et al.  Study of the levels of expression of two oncogenes, c-myc and c-myb, in acute and chronic leukemias of both lymphoid and myeloid lineage. , 1985, Leukemia research.