Influence of absorption in linear polarization imaging of melanoma tissues

The contrast mechanism of different polarization imaging techniques for melanoma in mouse skin is studied using both experiments and Monte Carlo simulations. Total intensity, linear polarization difference imaging (DPI), degree of polarization imaging (DOPI) and rotating linear polarization imaging (RLPI) are applied and the relative contrasts of these polarization imaging methods between the normal and cancerous tissues are compared. A two-layer absorption-scattering model is proposed to explain the contrast mechanism of the polarization imaging for melanoma. By taking into account of both scattering of symmetrical and asymmetrical scatterers and absorption of inter-scatterer medium, the two-layer model reproduces the relative contrasts for polarization images observed in experiments. The simulation results also show that, the parameters of polarization imaging change more dramatically with the variation of absorption in the bottom layer than the top layer.

[1]  G. Zonios,et al.  Simple two-layer reflectance model for biological tissue applications: lower absorbing layer. , 2010, Applied optics.

[2]  Nan Zeng,et al.  Penetration depth of linear polarization imaging for two-layer anisotropic samples. , 2011, Applied optics.

[3]  Dae-Duk Kim,et al.  Transdermal delivery of dideoxynucleoside-type anti-HIV drugs. 2. The effect of vehicle and enhancer on skin permeation. , 1996, Journal of pharmaceutical sciences.

[4]  Katherine W. Calabro,et al.  Gender variations in the optical properties of skin in murine animal models. , 2011, Journal of biomedical optics.

[5]  Yu. A. Ushenko,et al.  NEW PARAMETER FOR DESCRIBING AND ANALYZING THE OPTICAL-ANISOTROPIC PROPERTIES OF BIOLOGICAL TISSUES , 2011 .

[6]  Ken Lee,et al.  Design, testing, and clinical studies of a handheld polarized light camera. , 2004, Journal of biomedical optics.

[7]  Nan Zeng,et al.  Rotating linear polarization imaging technique for anisotropic tissues. , 2010, Journal of biomedical optics.

[8]  Claus Garbe,et al.  The natural course of cutaneous melanoma , 2004, Journal of surgical oncology.

[9]  Nan Zeng,et al.  POLARIZATION IMAGING AND SCATTERING MODEL OF CANCEROUS LIVER TISSUES , 2013 .

[10]  M. Raspanti,et al.  Collagen fibril structure is affected by collagen concentration and decorin. , 2007, Biomacromolecules.

[11]  Haishan Zeng,et al.  Front Matter: Volume 6842 , 2008 .

[12]  Angelo Pierangelo,et al.  Polarimetric imaging of uterine cervix: a case study. , 2013, Optics express.

[13]  Michael S. Feld,et al.  Imaging human epithelial properties with polarized light-scattering spectroscopy , 2001, Nature Medicine.

[14]  Wei Li,et al.  Monte Carlo simulation of polarized photon scattering in anisotropic media. , 2009, Optics express.

[15]  Robert R. Alfano,et al.  Emerging Optical Biomedical Imaging Techniques , 1996 .

[16]  S. Shapshay,et al.  Detection of preinvasive cancer cells , 2000, Nature.

[17]  J. G. Walker,et al.  Analysis of the spatial distribution of polarized light backscattered from layered scattering media. , 2002, Journal of biomedical optics.

[18]  Jessica C Ramella-Roman,et al.  Imaging skin pathology with polarized light. , 2002, Journal of biomedical optics.

[19]  Wei Li,et al.  Application of sphere-cylinder scattering model to skeletal muscle. , 2010, Optics express.

[20]  R. Anderson.  Polarized light examination and photography of the skin. , 1991, Archives of dermatology.

[21]  S Morgan,et al.  Polarization properties of light backscattered from a two layer scattering medium. , 2000, Optics express.

[22]  George C. Mantis,et al.  Simple two-layer reflectance model for biological tissue applications. , 2009, Applied optics.